Keywords
pcr
poscosecha
tiabendazole
imazalil
miclobutanil
anthracnose
pcr
postharvest
thiabendazole
imazalil
miclobutanil
How to Cite
Abstract
Molecular identification of the fungus Colletotrichum spp., isolated from banana (Musa spp) in high lands from Turrialba and determination of their sensitivity to postharvest fungicides. Anthracnose is one of the most serious banana post-harvest diseases. A total of 25 Colletotrichum isolates were obtained from banana fruits grown in 5 areas in Turrialba County, Costa Rica; 5 isolates per area. Isolates were characterized on the basis of polymerase chain reaction (PCR) using species- specific primers of the ITS region and ß-tubulin, gene, and their in vitro sensitivities to the fungicides thiabendazole, imazalil and myclobutanil at 0.1, 1.0, 10.0 y 100.0 μg.ml-1. All isolates were identified as Colletotrichum gloeosporioides and were sensitive to thiabendazole, whereas most isolates showed a marked reduction in sensitivity to imazalil and myclobutanil.
References
AKED S., KHAN H., MAGAN N. 2001. Control of the anthracnose pathogen of banana (Colletotrichum musae) using antioxidants alone and in combination with thiabendazole or imazalil. Plant Pathology. 50:601–608.
ALVINDIA D., NATSUAKI K. 2008. Evaluation of fungal epiphytes isolated from banana fruit surfaces for biocontrol of banana crown rot disease. Crop Protection (27):81200-1207.
Cai L., Hyde K., Taylor P., Weir B., Waller J., Abang M., Zhang J., Yang Y., Phoulivong S., Liu Z., Prihastuti H., Shivas G., McKenzie E., Johnston P. 2009. A polyphasic approach for studying Fungal Diversity 39:183–204.
CARRÉ V., STANGARLIN J., BECKER A., ZANELLA A., GONÇALVES JÚNIOR A., SCHWAN K., FRANZENER G., CRUZ M. 2006. Postharvest control of Colletotrichum musae in banana (Musa sp.) with camphor (Artemisia camphorata) and chitosan. Journal Scientia Agraria Paranaensis. Brasil. 5:57-66.
COLE T., COLE C., CONWAY E. 2005. Effectiveness of selected fungicides applied with or without surfactant in controlling anthracnose on three cultivars of Euonymus fortunei, Journal of Applied Horticulture. (7):16-19.
CHUNG H., CHUNG C., PENG., YANG R., HUANG W. 2010. Specific detection of benzamidazole resistance in Colletotrichum gloeosporioides from fruit crops by PCR-RFLP. Special Issue on Biocatalysis and Agriculture Biotechnology. 3:17-24.
DE LAPEYRE DE BELLAIRE L., CHILLET M., LASSOIS L., HAISSAM M. 2010. Crown rot of bananas: Preharvest factors involved in postharvest disease development and integrated control methods. Plant Disease. 94:648-658.
DE LAPEYRE DE BELLAIRE L., DUBOIS C. 1997. Distribution of thiabendazole-resistant Colletotrichum musae isolates from Guadalupe banana plantations. Plant Disease 81:1378-1383.
DEMERUTIS C., QUIRÓS L., MARTINUZ A., ALVARADO E., WILLIAMS R., ELLIS M. 2008. Evaluation of an organic treatment for post-harvest control of crown rot of banana. Ecological Engineering 3 4:324–327.
DU M., SCHRDL C., NUCKLES E., VAILLANCOURT L. 2005. Using mating-type gene sequence for improved phylogenetic resolution of Colletotrichum species complexes. Mycologia 97:641-658.
FREEMAN S., KATAN T. 1997. Identification of Colletotrichum species responsible for anthracnose and root necrosis of strawberry in Israel. Phytopathology 87:516-521.
GUERBER J., LIU B., CORRELL J., JOHNSTON P. 2003. Characterization of diversity in Colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtDNA and intron RFLPs, and mating compatibility. Mycologia 95:872-895.
GUZMÁN M., VILLALTA R. 2008. Eficacia de mezclas de azoxistrobina com tiabendazole para El combate de enfermedades poscosecha del banano (Musa AAA). CORBANA 34(61):1-10.
HERNÁNDEZ R. 2009. Sensibilidad a fungicidas poscosecha e identificación molecular del hongo Colletotrichum spp., agente causal de la antracnosis en frutos de banano en Costa Rica. Tesis de maestría. San José. Costa Rica. 96 p.
HYDE K., CAI L., CANNON F., CROUCH A., CROUS W., DAMM U., GOODWIN H., CHEN H., JOHNSTON R., JONES G., LIU Y., MCKENZIE C., MORIWAKI J., NOIREUNG P., PENNYCOOK R., PFENNING H., PRIHASTUTI H., SATO T., SHIVAS G., TAN P., TAYLOR J., WEIR S., YANG L. ZHANG, Z. 2009. Colletotrichum–names in current use. Fungal Diversity 39:147-182.
Hyde K., Cai L., McKenzie E., Yang Y., Zhang J., Prihastuti H. 2009. Colletotrichum: a catalogue of confusion. Fungal Diversity 39:1–17.
INFOSTAT. 2008. Software estadístico. Manual de usuario. Córdoba, Argentina.
JEGER M., EDEN-GREEN S., JOHANSON A., WALLER J., BROWN A.1995. Banana diseases. In: Banana and Plantains. Edited by Gowen. Chapman and Hall. London.UK.
KOLLER W., SCHEINPFLUG H. 1987. Fungal resistance to sterol biosynthesis inhibitors. Plant Disease. 71:1066-1074.
LASSOIS L., BASTIAANSE H., CHILLET M., JULLIEN A., JIJAKLI M., DE LAPEYRE DE BELLAIRE L. 2010. Hand position on the bunch and source–sink ratio influence the banana fruit susceptibility to crown rot disease. Annals of Applied Biology.156:221-229.
LIMA R., OLIVEIRA S., MENEZES M. 2003. Caracterização enzimática e patogenicidad cruzada de Colletotrichum spp., associados a doenças de póscolheita. Fitopatologia Brasileira 28:620-625.
LIU B., WASILVA L., MORELOCK T., O’NEIL N., CORREL J. 2007. Comparison of Colletotrichum orbiculare and several allied Colletotrichum spp., for mtDNA RFLPs, intron RFLP and sequence variation, vegetative compatibility, and host specificity. Phytopathology 97:1305-1314.
MAQBOOL M., ALI A., RAMACHANDRAN S., SMITH D., ALDERSON P.Gl. 2010. Control of postharvest anthracnose of banana using a new edible composite coating, Crop Protection doi:10.1016/j. cropro.2010.06.005.
MEDEIROS l., MACIEL D., MEDIEROS V., HOULLOU KIDO L., OLIVEIRA N. 2010. pelB gene in isolates of Colletotrichum gloeosporioides from several hosts. Genetics and Molecular Research 9:661-673.
MORIWAKI J., TSUKIBOSHI T., SATO T. 2002. Grouping of Colletotrichum species in Japan based on rDNA sequences. Journal Genetic Plant Pathology 68:307-320.
NIROSHINI W., KARUNARATNE A. 2009. Interactions of Colletotrichum musae and Lasiodiplodia theobromae and their biocontrol by Pantoea agglomerans and Flavobacterium sp., in expression of crown rot of ‘Embul’ banana. Biocontrol (NLD). 54:587-596.
PERES N., KURUMAE E., DIAS M., DE SOUZA N. 2002. Identification and characterization of Colletotrichum spp., affecting fruit after harvest in Brazil. Journal. Phytopathology. 150:128-134.
PÉREZ L., SÁENZ M., MAURI F. 2001. Control de la pudrición de la corona de los bananos en Cuba. I. Eficacia de benzimidazoles, imidazoles y triazoles. Fitosanidad 5:31-38.
Phoulivong S., CaI L., Chen H., McKenzie E., ABDELSALAM K., CHUKEATIROTE E., HYDE K.D. 2010. Colletotrichum gloeosporioides is not a common pathogen on tropical fruits. Fungal Diversity 44:33–43.
RAMIREZ C. 2010. “Ubicación Geográfica y Evaluación de la Calidad de Fruta de Banano de Altura que se produce en el Cantón de Turrialba, Costa Rica”. Tesis para licenciatura, Universidad de Costa Rica. Turrialba, Costa Rica. 69 p.
RAUT S., RANADE S. 2004. Diseases of banana and their management. Disease of Fruits and Vegetables. 2:37-57.
ROGERS S., BENDICH A. 1988. In: Plant Molecular Biology Manual. S. B. Gelvin y R. A. Schilperoort (eds.), Kluwer Academic Publishers, echt, The Netherlands. pp. A6/1-11. (Modificado por Sánche z-Chiang , N. 2009, Laboratorio de Técnicas Moleculares para la Fitoprotección, Centro de Investigaciones en Protección de Cultivos, Universidad de Costa Rica.
SCHILDER A., GILLET J., SYSAK R. 2001. Evaluation of Fungicides for Control of Anthracnose Fruit rot of blueberries fungicide and infanticide test. sp.
SHIVAS R., TAN Y. 2009. A taxonomic re-assessment of Colletotrichum acutatum, introducing C. fioriniae comb. et stat. nov. and C. simmondsii sp., nov. Fungal Diversity 39:111-122.
SOMBARDIER A., DUFOUR M., BLANCARD D., CORIOCOSTET M. 2010. Sensitivity of Podosphaera aphanis isolates to DMI fungicides: distribution and reduced cross-sensitivity. Pest Manag Sci 66:35–43.
SREENIVASAPRASAD S., SHARADA K., BROWN A., MILLS P. 1996. PCR-based detection of Colletotrichum acutatum on strawberry. Plant Pathology 45:650-655.
SREENIVASAPRASAD S., TALHINHAS P. 2005. Genotypic and phenotypic diversity in Colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts. Molecular Plant Pathology 6:361–378.
SUSUKI T., TANAKA C., EBIHARA Y., ITU Y., VEMATSU S. 2010. Genetic polymorphism and virulence of Colletotrichum gloeosporioides isolated from strawberry (Fragaria × ananassa Duchesne). Journal of General Plant Pathology.76:247-253.
UREÑA A., MACKENZIE S., BOWEN B., LEGARD D. 2002. Etiology and population genetics of Colletotrichum spp., causing crown and fruit rot of strawberry. Phytopathology, 92:1245-1252.
VALERO M., GARCÍA S., GINER M., ARANZAZU A., RUIZ J. 2010. Benomyl sensitivity assays and species-specific PCR reactions highlight association of two Colletotrichum gloeosporioides types and C. acutatum with rumple disease on Primofiori lemons. European Journal of Plant Pathology. 3:399-405.
VILLALTA R., SAMPLE M., SHIELDS D., GUZMÁN M. 2006. Evaluación de fungicidas y mezclas de fungicidas para el combate de enfermedades poscosecha del banano (Musa AAA). CORBANA 32(59):17-33.
WONG F., MIDLAND S., DE LA CERDA K. 2007. Occurrence and distribution of QoI-resistant isolates of Colletotrichum cereale from annual bluegrass in California. Plant Disease. 91:1531-1546.