Abstract
The activity of 4 soil enzymes in farms under different agronomic managements and in different soil types was analyzed. The enzymes tested were phosphatase, β-glucosidase, dehydrogenase and urease. Soil samples were taken from the first 20 cm in depth, in order to determine the activity of the 4 enzymes and analyze them chemically. It was found that Dehydrogenase enzyme activity ranged from 0.13 to 4.46 with an average of 1.17 (μg INTF.g - 1 .h -1 ); β-glucosidase enzyme ranged from 31.9 to 208.1 with an average of 108.1 (μg PNP.g -1 .h -1 ). Furthermore, phosphatase had values between 413.4 and 3043.6 with an average of 1521.5 μg PNP.g -1 .h -1 , while Urease ranged from 12.5 to 52.8 with an average of 38.3 μg N-NH4.g -1 .h -1 . Phosphatase correlated with the percentage of organic matter and carbon in the soil (0.75 and 0.76 respectively, p<0.01); an inverse relationship was found between the activity of this enzyme and the content of available P (-0.53r, significant at p<0.10). Urease correlated with phosphatase (r=0.61 p<0.05) and with dehydrogenase (r-0.77 p<0.01). Multivariate cluster analysis based on the enzymatic activity allowed to make similar groups according to the type of crop, which shows the potential of usage that this property has for undertaking more detailed studies.References
ALEF K., NANNIPIERI P. 1995. Methods in applied soil microbiology and biochemistry. Academic Press. 576 p.
ALEXANDER M. 1980. Introduction to Soil Microbiology. 2ª Ed. John Wiley and Sons, Inc. New York, USA. 467 p.
BALEŽENTIENĖ L. 2012. Hydrolases Related to C and N Cycles and Soil Fertility Amendment: Responses to Different Management Styles of Agro-Ecosystems. Pol. J. Environ. Stud. 21(5):1153-1159.
BALEŽENTIENĖ L., KLIMAS E. 2009. Effect of organic and mineral fertilizers and land management on soil enzyme activities. Effect of organic and mineral fertilizers and land management on soil enzyme activities (Special issue I) 9:191-197.
BOLTON J.R., ELLIOT L.F., PAPENDICK R.I., BEZDICEK D.F. 1985. Soil microbial biomass and selected soil enzyme activities: effect of fertilization and cropping practices. Soil Biology and Biochemistry 17:297-302.
BREMER J.M., TABATABAI M.A. 1971. Use of automated combustion techniques for total carbon, total nitrogen and sulfru analysis of soils, pp. 1-6. In: l.M. Walsch (ed.) Intrumental methods for analysis and plant tissues.
CALDWELL B.A. 2005. Enzyme activities as a component of soil biodiversity: a review. Pedibiología 49:637-644.
CARAVACA F., MASCIANDARO G., CECCANTI B. 2002. Land use in relation to soil chemical and biochemical properties in a semiarid Mediterranean environment. Soil and Tillage Research 68(1):23-30.
CARPA R. 2009. Enzymological research on soils from different environments. Annals of RSCB 16(1):44-48.
CERÓN L., MEIGAREJO L. 2005. Enzimas del suelo: indicadores de salud y calidad. Acta Biológica Colombiana 10(1):5-18.
COYNE M. 2000. Microbiología del suelo: un enfoque exploratorio. Editorial Paraninfo, España. 416 p.
DIAZ-ROMEU R., HUNTER A. 1978. Metodologías de muestreo de suelos, análisis químico de suelos y tejido vegetal y de investigación en invernadero. CATIE, Turrialba, Costa Rica. 62 p.
DICK W.A., TABATABAI M.A. 1993. Significance and potencial uses of soil enzymes. Soil Microbial Ecology Meeting, pp. 95-127. Jr., F.B. (ed.). Soil Microbial Ecology. Marcel Dekker, New York.
FERRERAS L., TORESANI S., BONEL B., FERNÁNDEZ E., BACIGALUPPO S., FAGGIOLI V., BELTRÁN C. 2009. Parámetros químicos y biológicos como indicadores de calidad del suelo en diferentes manejos. CI. SUELO (ARGENTINA) 27(1):103-114.
GAJDA A., MARTYNIUK S. 2005. Microbial Biomass C and N and Activity of Enzymes in Soil under Winter Wheat Grown in Different Crop Management Systems. Polish Journal of Environmental Studies 14(2):159-163.
GIL-SOTRES F., TRASAR-CEPEDA C., LEIRÓS M.C., SEOANE S. 2005. Different approaches to evaluating soil quality using biochemical properties. Soil Biology and Biochemistry 37(5):877-887.
HENRIQUEZ C., CABALCETA G. 2012. Guía práctica para el estudio introductorio de los suelos con un enfoque agrícola. Asociación Costarricense de la Ciencia del suelo. San José Costa Rica. 111 p.
HU Y.L., WANG S.L., ZENG D.H. 2006. Effects of Single Chinese Fir and Mixed Leaf Litters on Soil Chemical, Microbial Properties and Soil Enzyme Activities. Plant and Soil 282(1-2):379-386.
KANDELER E., STEMMER M., KLIMANEK E.M. 1999. Response of soil microbial biomass, urease and xylanase within particle size fractions to long-term soil management. Soil Biology and Biochemistry 31(2):261-273.
KUHUR M., GARTIA S.K., PATEL A.K. 2012. Quantifying the contribution of diferente soil properties on enzyme activities in dry tropical ecosystems. Journal of Agricultural and Biological Science 7(9):763-773.
MARCOTE I., HERNÁNDEZ T., GARCÍA C., POLO A. 2001. Influence of one or two successive annual applications of organic fertilizers on the enzyme activity of a soil under barley cultivation. Biorecourse Technology 79:147-154.
PAUL E.A., CLARK F.E. 2007. Soil Microbiology and Biochemistry. Academic Press. 275 p.
PAZ-FERREIRO J., TRASAR-CEPEDA C., LEIRÓS M.C., SEOANE S., GIL-SOTRES F. 2007. Biochemical properties of acid soils under native grassland in a temperate humid zone. New Zealand Journal of Agricultural Research 50(4):537-548.
TRASAR C., LEIROS M., GIL F. 2003. Consideraciones generales sobre la determinación de las actividades enzimáticas del suelo. In: Técnicas de análisis de parámetros bioquímicos en suelos. Ed. C. García, F. Gil, T. Hernándes y C. Trasar. España. Mundiprensa. 371 p.
UZUN N., UYANÖZ R. 2011. Determination of Urease Catalase Activities and CO2 Respiration in Different Soils Obtained From in Semi Arid Region Konya, Turkey. Trends in Soil & Plant Sciences Journal 2(1):1-6.