Keywords
híbrido f1
café
jiffy
invernadero
in vitro
rita
aclimatization
hybrid f1
coffee
jiffy
green house
in vitro
rita
How to Cite
Abstract
F1 Hybrid seedlings, obtained by indirect somatic embryogenesis, were acclimatizated under greenhouse conditions, separated in 2 types of container: biodegradable mesh with compressed turf (Jiffy®) and the other on conventional tray with soil and rice husk. Two months later, the plantlets were transferred to bags with conventional substrate and grown under nursery conditions. Development and quality of the plants were evaluated after growing during 8 months under conventional management. It was found that 33% had straight roots, however 65% had abnormalities, such as constrictions and loops, twists, bifurcations, among others, corresponding to lesser values in length, thickness and weight, both in the radical system as well as aerial. In regard to container type, acclimatizated plants grown in Jiffy® showed better growth than those in trays; however, those on which the cover was kept during the nursery stage were less developed, as compared to those were the cover was removed. It is recommended to remove the Jiffy® cover before planting in the soil bag in the nursery, and improve the temporary immersion technique to prevent morphological abnormalities of the embryos produced.References
AZOFEIFA J., ROJAS A., HINE A. 2009. Optimización del proceso de enraizamiento y aclimatización de vitroplantas de Swietenia macrophylla King (Orden: Meliacea). Tecnología en Marcha 22(3):34-41.
BARRY-ETIENNE D., BERTRAND B., VASQUES N., ETIENNE H. 2002(a). Comparison os fomatic embryogenesis-derived Coffee (Coffea arábica L.) plantlets regerated in vitro or ex vitro: Morphological, Mineral and Water Characteristics. Annals of Botanny 90:77-85.
BARRY-ETIENNE D., BERTRAND B., SCHLÖNVOIGHT A., ETIENNE H. 2002(b). The morphologycal variability within a population of coffee somatic embryos produced ina biorreactor affects the regeneration and the developmente of plants in the nursery. Plant Cell, Tissue and Organ Culture 68:153-162.
BOXTEL J., BERTHOULY M. 1995. High frequency somatic embryogenesis from coffee leaves. Plant Cell, Tissue and Organ Culture 44:7-17.
BERTHOULY M., MICHAUX-FERRIERE N.M. 1996. High frequency somatic embryogenesis from Coffea canephora. Plant Cell, Tissue and Organ Culture 44:169-176.
COSTA J., FERREIRA E., PASQUAL M., VILLA F., BOTELHO C., PEREIRA S. 2008. Development of Coffea Arabica L., seedlings obtained form direct somatic embryogenesis. Coffee Science 3(1):30-37.
DE LOS SANTOS C., HERNÁNDEZ T. 2006. Coffee Biotechnology. Braz. J. Plant Physiol. 18(1):217-227.
ETIENNE H., ANTHONY F., DUSSERT S., FERNANDEZ D., LASHERMES P., BERTRAND B. 2002. Biotechnological applications for the improvement of Coffee (Coffea arabica L.). In Vitro Cell. Dev. Biol. Plant. 38:129-138.
ETIENNE H., BARRY-ETIENNE D., VASQUEZ N., BERTHOULY M. 1999. Aportes de la biotecnologia al mejoramiento genético del café: el ejemplo de la multiplicacion por embriogénesis somatica de hibridos F1 en America Central, pp. 457-493. In: B. Bertrand y B. Rapidel (eds.). Desafios de la caficultura en Centroamerica. San Jose: IICA.
ETIENNE H., SOLANO W., PEREIRA A., BARRY D., BERTRAND B., ANTHONY F., CÔTE F., BERTHOULY M. 1998. La propagación masal de los híbridos F1 de cafés arábigos. Boletin PROMECAFE (80):12-15.
GATICA A., ARRIETA G., ESPINOZA A. 2007. Comparison of three in vitro protocols for direct embryogenesis and plant regeneration of Coffea arabica L. Cvs. Caturra and Catuaí. Agronomía Costarricense 31(1):85-94.
LEBUDE A., GOLDFAED B., BLAZICH F., WRIGHT J., CAZELL B., WIZE F., FRAMPTON J. 2006. Container type and volumen influences adventitious rooting and subsequent field growth of stem cuttings of Loblolly pine. SJAF. 30(30):123-131.
ROJAS F., ARIAS D., MOYA R., MEZA A., MURILLO O., ARGUEDASM. 2004. Manual para productores de melina Gmelina arbórea en Costa Rica. Cartago. 314 p.
STARITSKY G. 1970. Embryoid formation in callus tissues of coffee. Acta Bot. Need. 19:509-514.
SÖNDAHL M., SHARP W., 1977. High frecuency induction of somatic embryos in cultured leaf explants of Coffea arabica L. Zeitschriff fûr Pflanzenphysiologie. 81:395-408.
YASUDA T., FUJII Y., YAMAGUCHI T. 1985. Embryogenic callus induction from Coffea arabica leaf explants by benzyladenine. Plant Cell Physiol. 26:595-597.