Keywords
cultivo in vitro
musa sp.
cv. cavendish
sulpomag
micropropagation
in vitro culture
musa sp.
cv. cavendish
sulpomag
How to Cite
Abstract
The Sulpomag natural fertilizer, or potassium sulfate and magnesium, constituted by K 2 O 22%, MgO 22%, S 18% and Cl (chloride) 2.5% plus organic complexes of coconut water 10 to 20%, and casein hydrolyzate 250 and 500 mg.l -1 , as partial substitutes for mineral salts of Murashige and Skoog (MS), were used in the in vitro propagation of banana, Musa sp., cv. Cavendish. The aim was that the in vitro plants grow and develop in minimum quantities of mineral salts, banned in the technical regulation for organic products. Shoot tips, established in vitro on MS medium, were transferred to 10 treatments for shoot multiplication supplemented with KH 2 PO 4 (170 mg.l -1 ), CaCl 2 .2H 2 O (150 mg.l -1 ), NH 4 NO 3 and KNO 3 , at 1/5 and 1/10 of the MS concentration, MS micronutrients and 0.1096 g.l -1 of Sulpomag, IAA (0.2 mg.l -l ) and BAP (5 mg.l -1 ). The rooting medium was the same formulation of mineral salts, only varying on the growth regulators. Best results were obtained in culture media supplemented with NH 4 NO 3 and KNO 3 at 1/5 of MS concentration, 0.1096 g.l -1 Sulpomag and coconut water 10 y 20%. The results allow to consider the possibility of using Sulpomag and coconut water, as partial substitutes for MS mineral salts for in vitro propagation of Musa sp., cv. Cavendish for use in organic agriculture.References
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