Agronomía Costarricense ISSN Impreso: 0377-9424 ISSN electrónico: 2215-2202

OAI: https://revistas.ucr.ac.cr/index.php/agrocost/oai
Effectiveness of formic acid and thymol in the control of <i>Varroa destructor</i> in africanized honey bee colonies
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Keywords

ácido fórmico
timol
varroa destructor
abejas africanizadas
formic acid
thymol
varroa destructor
africanized honeybees

How to Cite

Calderón, R. A., Ramírez, M., Ramírez, F., & Villalobos, E. (2014). Effectiveness of formic acid and thymol in the control of <i>Varroa destructor</i> in africanized honey bee colonies. Agronomía Costarricense, 38(1). https://doi.org/10.15517/rac.v38i1.15165

Abstract

The effectiveness of formic acid and thymol in the control of the mite Varroa destructor was evaluated from June to October 2011 in an apiary of 16 Africanized honeybee colonies (the colonies did not receive chemical treatment at all) located in Atenas, Alajuela. The apiary was randomly divided in three groups (A, B and C) of 8, 5 and 3 colonies, respectively. Group A was treated with 150 g of formic acid at 65%, group B received 2 applications of 25 g thymol and colonies of group C were used as control. Varroa traps were placed on the bottom board of each colony to collect the mites. After 4 weeks, all colonies were treated with 4 strips of flumethrin to kill the remaining mites and to assess the effectiveness of the products. Furthermore, the mortality of the different stages of varroa was evaluated in capped worker brood cells checking the pupa and the bottom of the cell for mites. The effectiveness of formic acid and thymol in the control of varroa mites was 94.7% and 96.9% respectively. In colonies treated with formic acid varroa mortality occurred mainly during 72 h after application, meanwhile in the roup treated with thymol the mite mortality was observed mainly one week after treatment. In addition, in the group of colonies treated with formic acid 86% of mite mortality occurred in capped brood cells, while in colonies treated with thymol and control colonies the varroa mortality in capped cells was less than 10%. In conclusion, the effectiveness of formic acid and thymol in the control of varroa mites was high, for which reason both products can be considered an alternative for the integrated management of varroa mites in Africanized honeybees colonies.
https://doi.org/10.15517/rac.v38i1.15165
PDF (Español (España))

References

ADAMCZYK S., LÁZARO R., PÉREZ-ARQUILLUÉ C., CONCHELLO P., HERRERA A. 2005. Evaluation of residues of essential oil components in honey after different anti-Varroa treatments. Journal of Agricultural and Food Chemistry 53:10085-10090.

AL-RIFAI J., AKEEL N. 1997. Determination of pesticide residues in imported and locally produced honey in Jordan. Journal of Apicultural Research 36:155-161.

AMRINE J., NOEL R., WEBB D. 2007. Results of 50% formic acid fumigation of honey bee hives [Apis mellifera ligustica (Hymenoptera: Apidae)] to control varroa mites (Acari: varroidae) in brood combs in Florida, U.S.A. International Journal of Acarology 2:99-109.

BAGGIO A., ARCULEO P., NANETTI A., MARINELLI E., MUTINELLI F. 2004. Field trials with different thymol-based products for the control of Varroosis. American Bee Journal 144:395-400.

BAHREINI R., TAHMASEBI G., NOWZARI J., TALEBI M. 2004. A study of the efficacy of formic acid in controlling Varroa destructor and its correlation with temperature in Iran. Journal of Apicultural Research 43:158-161.

BAILEY L., BALL B. 1991. Honey Bee Pathology. 2 ed. Academic Press, London, UK. 193 p. BOGDANOV S. 2006. Contaminants of bee products. Apidologie 37:1-18.

BÜCHLER R., BERG S., LE CONTE Y. 2010. Breeding for resistance to Varroa destructor in Europe. Apidologie 41:393-408.

CALDERÓN R.A. 2009. Diagnóstico de enfermedades de la cría en abejas africanizadas en Costa Rica. Memorias del X Congreso Nacional de Apicultura: Apicultura y su impacto en la seguridad alimentaria. San José, CR. 66 p.

CALDERÓN R.A. 2011. Reducción de la población de ácaros de varroa en colmenas de abejas Africanizadas utilizando una trampa de fondo. Boletín de Parasitología 12:2-4.

CALDERÓN R.A., ARCE H., VAN VEEN J. 1998. Detección, distribución y control de Varroa jacobsoni Oudemans en Costa Rica. Ciencias Veterinarias 21:31-40.

CALDERÓN R.A., FALLAS N., SÁNCHEZ L. 2007. Detección de enfermedades en abejas Africanizadas en Costa Rica. Ciencias Veterinarias 25:335-348.

CALDERÓN R.A., ORTIZ R., ARCE H., VAN VEEN J., QUAN J. 2000b. Effectiveness of formic acid on varroa mortality in capped brood cells of Africanized honey bees. Journal of Apicultural Research 39:177-179.

CALDERÓN R.A., ORTIZ R., SÁNCHEZ L., LALAMA K. 2000a. Control del ácaro Varroa jacobsoni en abejas melíferas (Apis mellifera) bajo condiciones tropicales. Ciencias Veterinarias 23:45-55.

CALDERÓN R.A., VAN VEEN J., ARCE H., RAMÍREZ M. 1999. Use of formic acid for varroa mites control in the tropical conditions of Costa Rica. Ciencias Veterinarias 22:21-27.

CALDERONE N. 2010. Evaluation of Mite-Away-II™ for fall control of Varroa destructor (Acari:Varroidae) in colonies of the honey bee Apis mellifera (Hymenoptera: Apidae) in the northeastern USA. Experimental and Applied Acarology 50:123-132.

CALIS J., BOOT W., BEETSMA J., VAN DEN EIJNDE J., RUIJTER A., VAN DER STEEN J. 1998. Control of varroa combining trapping in honey bee worker brood with formic acid treatment of the capped brood outside the colony: putting knowledge on brood cell invasion into practice. Journal of Apicultural Research 37:205-215.

DONZÉ G., GUERIN P. 1994. Behavioral attributes and parental care of varroa mites parasiting honey bee brood. Behavioral Ecology and Sociobiology 34:305-319.

EGUARAS M. 2003. El ácido fórmico como agente de control de Varroa destructor en Argentina. Vida Apícola 122:36-42.

EGUARAS M., DEL HOYO M., PALACIO M., RUFFINENGO S., BEDASCARRASBURE E. 2001a. A new product with formic acid for Varroa jacobsoni Oud. Control in Argentina. I. Efficacy. The Journal of Veterinary Medical Science 48:11-14.

EGUARAS M., LABATTAGLIA M., FAVERIN C., DEL HOYO M., PALACIO M., CARRIN A., RUFFINENGO S., BEDASCARRABURE E. 2001b. Varroa jacobsoni control with formic acid used in different application ways in subtropical and temperate climates. Apiacta 36:97-101.

EGUARAS M., PALACIO M., FAVERIN C., BASUALDO M., DEL HOYO M., VELIS G., BEDASCARRASBURE E. 2003. Efficacy of formic acid in gel for varroa control in Apis mellifera L.: importance of the dispenser position inside the hive. Veterinary Parasitology 111:241-245. EMSEN B., GUZMÁN-NOVOA E., KELLY P. 2007. The effect of three methods of application on the efficacy of thymol and oxalic acid for the fall control of the honey bee parasitic mite Varroa destructor in a northern climate. Journal of Apicultural Research 147:535-539.

ESPINA D., ORDETX G. 1984. Apicultura tropical. Tecnológica de Costa Rica, Cartago, CR. 506 p.

ESPINOSA L., GUZMÁN E. 2007. Eficacia de dos acaricidas naturales, ácido fórmico y timol, para el control del ácaro Varroa destructor de las abejas (Apis mellifera L.) en Villa Guerrero, Estado de México, MX. Veterinaria México 38:9-19.

FELDLAUFER M., PETTIS J., KOCHANSKY J., SHIMANUKI H. 1997. A gel formulation of formic acid for the control of parasitic mites of honey bees. American Bee Journal 137:661-663.

GIOVENAZZO P., DUBREUIL P. 2011. Evaluation of spring organic treatments against Varroa destructor (Acari: Varroidae) in honey bee Apis mellifera (Hymenoptera: Apidae) colonies in eastern Canada. Experimental and Applied Acarology 55:65-76.

GONZÁLEZ A., ABARCA D., MARCANGELI J., MORENO L., AGUAYO O. 2005. Comparación de la eficacia del ácido fórmico y del fluvalinato, como métodos de control de Varroa destructor (Acari: Varroidae) en colmenas de Apis mellifera (Hymenoptera: Apidae), en Ñuble, centro sur de Chile. Revista de la Sociedad Entomológica Argentina 64:35-42.

GREGORC A., PLANINC I. 2005. The control of Varroa destructor in honey bee colonies using the thymolbased acaricide- Apiguard. American Bee Journal 145:672-675.

HARBO J., HARRIS J. 2005. Suppressed mite reproduction explained by the behavior of adult bees. Journal of Apicultural Research 44:21-23.

IFANTIDIS M.D. 1997. Ontogenesis of Varroa jacobsoni Oud. In Cahiers Options Méditerranéennes. Varroosis in the Mediterranean region. CIHEAM, Zaragosa, ES. p 13-21.

JUNKES L., VIEIRA J., GUERRA J., MORETTO G. 2007. Varroa destructor mite mortality rate according to the amount of worker brood in Africanized honey bee (Apis mellifera L.) colonies. Acta Scientiarum Biological Sciences 29:305-308.

KANGA L., ADAMCZYK J., MARSHALL K., COX R. 2010. Monitoring for resistance to organophosphorus and pyrethroid insecticides in varroa mite populations. Journal of Economic Entomology 5:1797-1802.

KOENIGER N., FUCHS S. 1988. Control of Varroa jacobsoni Oud. in honeybee colonies containing sealed brood cells. Apidologie 19:117-130.

LOUCIF-AYAD W., ARIBI N., SMAGGHE G., SOLTANI N. 2010. Comparative effectiveness of some acaricides used to control Varroa destructor (Mesostigmata: Varroidae) in Algeria. African Entomology 18:259-266.

MARTIN S. 1994. Ontogenesis of the mite Varroa jacobsoni Oud in worker brood of the honeybee Apis mellifera L. under natural conditions. Experimental and Applied Acarology 18:87-100.

MARTIN S. 1995. Ontogenesis of the mite Varroa jacobsoni Oud in drone brood of the honeybee Apis mellifera L. under natural conditions. Experimental and Applied Acarology 19:199-210.

MARTIN S., HOLLAND K., MURRAY M. 1997. Nonreproduction in the honey bee mite Varroa jacobsoni in honey bee (Apis mellifera) colonies. Journal of Apicultural Research 36:113-123.

MILANI N. 1995. The resistance of Varroa jacobsoni Oud to pyrethroids: a laboratory assay. Apidologie 23:257-272.

MITCHELL D., VANDERDUSSEN D. 2010. Mite away quick strip™ mite honey flow efficacy trial. American Bee Journal 150:487-489.

MORETTO G. 1997. Defense of Africanized bee workers against the mite Varroa jacobsoni in Southern Brazil. American Bee Journal 137:746-747.

MORETTO G., GONCALVES L., DE JONG D. 1991. Africanized bees are more efficient at removing Varroa jacobsoni, preliminary data. American Bee Journal 131:434.

MORETTO G., LEONIDAS J. 1999. Varroa jacobsoni infestation of adult Africanized and Italian honey bees (Apis mellifera) in mixed colonies in Brazil. Genetics and Molecular Biology 22:321-323.

MORETTO G., LEONIDAS J. 2003. Infestation and distribution of the mite Varroa destructor in colonies of Africanized bees. Brazilian Journal of Biology 63:83-86.

PALMERI V., CAMPOLO O., ZAPPALA L. 2007. Evaluation of two methods for applying Apiguard® in an area with continuos nectar flows and brood rearing. Journal of Apicultural Research 46:105-109.

RINDERER T., HARRIS J., HUNT G., GUZMAN L. 2010. Breeding for resistance to Varroa destructor in North America. Apidologie 41:409-424.

RITTER W. 1993. Chemical control: options and problems. In Matheson A. (ed) Living with varroa. The International Bee Research Association (IBRA), Cardiff, UK. p. 17-24.

RITTER W. 2001. Enfermedades de las abejas. Acribia, Zaragoza, ES. 146 p.

SATTA A., FLORIS I., EGUARAS M., CABRAS P., GARAU V., MELIS M. 2005. Formic acid-based treatments for control of Varroa destructor in a mediterranean area. Journal of Economic Entomology 98:267-273.

SPIVAK M., REUTER G. 2001. Varroa destructor infestation in untreated honey bee (Hymenoptera: Apidae) colonies selected for hygienic behavior. Journal of Economic Entomology 94:326-331.

TROUILLER J. 1998. Monitoring Varroa jacobsoni resistance to pyrethroids in western Europe. Apidologie 29:537-546.

TROUILLER J. 2004. Apiguard: an instrument adapted to many beekeeping conditions. Apiacta 38:328-333.

TSIGOURI A., MENKISSOGLU-SPIROUDI U., THRASYVOULOU A., DIAMANTIDIS G. 2003. Fluvalinate residues in greek honey and beeswax. Apiacta 38:50-53.

UREÑA S. 2009. Reproducción del ácaro Varroa destructor (Mesostigmata: Varroidae) en celdas de cría de obrera y zángano en abejas africanizadas (Apis mellifera). Tesis de licenciatura, Universidad Nacional, Heredia, Costa Rica. 64 p.

VAN ALTEN A., TAM J., KEMPERS M. 2009. Winter colony health assessment after using Mite Away™ Quick Strip (MAQS™) as a control for varroa mites in the fall of 2009. Ontario Beekeepers association, CA. 5 p.

VANDAME R. 2000. Control alternativo de varroa en apicultura. El Colegio de la Frontera Sur (ECOSUR). Chiapas, MX. 30 p.

VILLALOBOS E. 2010. Varroa treatment recommendations with formic acid. University of Hawaii, honey bee varroa mite project. Hawaii, US. 4 p.

WALLNER K. 1999. Varroacides and their residues in bee products. Apidologie 30:235-248.

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