Micropropagation of Philodendron sp. (Probably P. corcovadense)

Abstract

Protocols for in vitro growth and multiplication, as well as for greenhouse acclimatization of this plant, were developed. In vitro establishment was successfully achieved in a Murashigue and Skoog (MS)(1962) medium supplemented with 0.8 mg l^-1 of N6-bencil- adenine (BA), 2 mg l^-1 of 3-indol-acetic acid (IAA), 30 g l^-1 of sucrose and 8 g l^-1 of agar. For multiplication, the explants were cultured on MS medium supplemented with 160 mg l^-1 of adenine sulfate, 170 mg l^-1 of NaH₂PO₄.H₂O and 53 combinations of BA, 6-furfurylaminopurine (KIN), N⁶-(∆²-isopentenyl) adenine (2-ip), IAA, and α-naphtalene acetic acid (NAA). The highest rate of shoot multiplication, 3 shoots every four weeks, was achieved on MS medium containing 160 mg l^-1 of adenine sulfate, 170 mg l^-1 of NaH₂PO₄.H₂O, 7mgl^-1 of BA, 3 mgl^-1 of KIN. In vitro rooting occurred on a hormone- free medium. Plantlets were successfully accli- matized in the greenhouse.