The objective of this study was to determine the number of spores of fungi that cause diseases in the fruits of papaya and to measure the influence that petiole elimination could have on the population of disease propagating agents. The stems collected during the rainy season released a higher number (82%) of Colletotrichum sp. conidia than those collectedduring the dry season. In the stems collected during the rainy season a reduction was observed in the amount of conidia released (77%), between the first and the second week after collection. The stems collected during the dry and rainy season released a small amount of conidia after four weeks of collection. The higher numbers of Glomerella sp. ascospores captured during April to June. The capture of Colletotrichum sp. conidia with and without elimination of stems showed values that increased from April to June with a maximum between September and October. The numbers of disease propagating agents captured in the plots with and without stem elimination were similar for Glomerella sp. The elimination of stems caused a reduction in the number of spores captured in Colletotrichum sp., Helminthosporium sp. and Fusarium sp. fungi. Stem elimination caused no effect on Curvularia sp. and Alternaria sp. fungi. The rainy season was more favorable for the collection of spores of Helminthosporium sp., Curvularia sp. and Fusarium sp., compared to that from the dry season. For Alternaria sp. a higher number of spore was collected during the dry season.