Molecular characterization of Fusarium associated with basal rot of thefruit of pitahaya (Selenicereus megalanthus).
DOI:
https://doi.org/10.15517/am.v27i2.21269Keywords:
phylogeny, rot of the fruit, etiology.Abstract
The aim of this study was to identify pathogenic isolates of Fusarium in fruits of yellow pitahaya affected by basal rot. Molecular characterization was performed on seventeen isolates associated with the disease, collected in yellow pitahaya producing areas of Valle del Cauca, Risaralda, Boyacá, and Cundinamarca in Colombia. PCR with primers ITS1 - ITS4 and TEF1α was used. The sequences of the PCR products were compared with the ones registered in National Center for Biotechnology Information (NCBI) and were aligned using Clustal W2 program, the phylogenetic relationships were built with MEGA6 program with the coefficient of maximum likelihood. The sequencing results for the markers showed that pathogenic isolates were F. oxysporum. Phylogenetic concept confirmed that basal rot of yellow pitahaya was caused by F. oxysporum. A species of Fusarium not reported in GenBank was also identified.
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References
Agrios, G. 2005. Plant pathology. 5th ed. University of Florida, FL, USA.
Alves-Santos, F.M., L. Cordeiro-Rodrigues, J.M. Sayagues, R. Martín-Domínguez, P. García-Benavides, M.C. Crespo, M. Díaz-Mínguez, and A.P. Eslava. 2002. Pathogenicity and race characterization of Fusarium oxysporum f. sp. phaseoli isolates from Spain and Greece. Plant Pathol. 51:605-611. doi:10.1046/j.1365-3059.2002.00745.x
Arciniegas-León, P. A., J. Torres-Cleves, y A. Abrajim-Pérez. 2014. Estudio de factibilidad para la exportación de pitahaya amarilla al mercado de Japón, como uno de los países líderes de la cuenca del Pacífico. Tesis de grado. Universidad del Rosario, Bogotá, COL.
Balajee, S., A. Borman, M. Brandt, J. Cano, M. Cuenca- Estrella, and E. Dannaoui. 2009. Sequence-based identification of Aspergillus, Fusarium, and mucorales species in the clinical mycology laboratory. J. Clin. Microbiol. 47:877-884.
Barthana, D., and N. Nasir. 2013. Description of symptoms and disease severity of yellow disease stem rot of red dragon fruit (Hylocereus polyrhizus, L.) in Padang Pariaman, West Sumatera. J. Bio. UA. 2:222-228.
Biblikiowicz, A., y S.M. Hernández. 1998. Organismos Fungosos presentes en las estructuras reproductivas de la pitahaya amarilla. Trabajo de grado, Universidad Nacional de Colombia, Santa Fé de Bogotá, COL.
Boyd, L., C. Ridout, D. O’Sullivan, J. Leach, and H. Leung. 2013. Plant–pathogen interactions: disease resistance in modern agricultura. Trends Genet. 29:233-240. doi:10.1016/j.tig.2012.10.011
Castillo, R., M. Livera, y G. Márquez. 2005. Caracterización morfológica y compatibilidad sexual de cinco genotipos de pitahaya (Hylocereus undatus). Agrociencia 39:183-194.
Choi, H.W., I.M. Chung, M.H. Sin, Y.S. Kim, J.B. Sim, J.W. Kim, and S.C. Chun. 2007. The effect of spent mushroom sawdust compost mixes, calcium cyanamide and solarization on basal stem rot of the cactus Hylocereus trigonus caused by Fusarium oxysporum. Crop Prot. 26: 162-168. doi:10.1016/j.cropro.2006.04.017
Creucí, M., F. Otálvaro-Tamayo, J.E. Muñoz, J.G. Morales, R.S. Suárez, C. Sandoval, M.A. Martínez, D. Cañar, R. Peña, É. Parra, E. Muñoz, R. Rojas, J. Jiménez, A. Benavides, y L.F. Pérez. 2011. Enfoque multidisciplinario para solución en el agro colombiano: el caso pitahaya amarilla Selenicereus megalanthus. Rev. Asoc. Col. Cienc. Biol. 23:52-64.
Daboussi, M.J., T. Langin, and Y. Brygoo. 1992. Fot1, a new family of fungal transposable elements. Mol. Gen. Genet. 232: 12-16. doi:10.1007/BF00299131
Delgado-Millán, J.A. 2012. Análisis general de mercado de la pitahaya amarilla (Selenicereus megalanthus), con fines de su comercialización en la República Popular China (RPC). Tesis de grado, Universidad Autónoma de Occidente, COL.
Edel, V., N. Steinberg, G. Gautheron, C. Recorbet, and C. Alabouvette. 2001. Genetic diversity of Fusarium oxysporum populations isolated from different soils in France. FEMS Microbiol. Ecol. 36:61-71. doi:10.1146/annurev.phyto.40.030402.110010
Geiser, D., T. Aoki, C.W. Bacon, S.E. Baker, M.K. Bhattacharyya, M.E. Brandt, D.W. Brown, L.W. Burgess, S. Chulze, J.J. Coleman, J.C. Correll, S.F. Covert, P.W. Crous, C.A. Cuomo, G. Sybren-De Hoog, A. Di Pietro, W.H. Elmer, L. Epstein, R.J.N. Frandsen, S. Freeman, T. Gagkaeva, A.E. Glenn, T.R. Gordon, N.F. Gregory, K.E. Hammond-Kosack, L.E. Hanson, M. Jímenez-Gasco, S. Kang, H. Corby-Kistler, G.A. Kuldau, J. Leslie, A. Logrieco, G. Lu, E. Lysøe, L. Ma, S.P. McCormick, Q. Migheli, A. Moretti, F. Munaut, K. O’Donnell, L. Pfenning, R.C. Ploetz, R.H. Proctor, S.A. Rehner, V.A.R.G. Robert, A.P. Rooney, B. Salleh, M. Scandiani, J. Scauflaire, D.P.G. Short, E. Steenkamp, H. Suga, B. A. Summerell, D.A. Sutton, U. Thrane, F. Trail, A. Van Diepeningen, H.D. VanEtten, A. Viljoen, C. Waalwijk, T.J. Ward, M.J. Wingfield, J. Xu, X. Yang, T. Yli-
Mattila, and N. Zhang. 2013. One fungus, one name: defining the genus Fusarium in a scientifically robust way that preserves longstanding use. Phytopathol. 103:400-408. doi:10.1094/PHYTO-07-12-0150-LE
Geiser, D., M. Jiménez-Gasco, S. Kang, I. Makalowska, N. Veeraraghavan, J. Ward, and K. O’Donnell. 2004. FUSARIUM-ID v. 1.0: a DNA sequence database for identifying Fusarium. Eur. J. Plant Pathol. 473-479. DOI: 10.1007/978-1-4020-2285-2_2
Gómez, E. 2008. Caracterización de cepas toxigénicas del género Fusarium mediante técnicas de biología molecular. Tesis PhD., Universidad Politécnica de Valencia, ESP.
Jacobson, D., and T. Gordon. 1991. Fusarium oxysporum f. sp. melonis: a case study of diversity within a formae specialis. Phytopathol. 81:1064-1067.
Kistler, H.C. 1997. Genetic diversity in the plant- pathogenic fungus Fusarium oxysporum. Phytopathology 87: 474-479.
Kondo, T., M. Martínez, J.A. Medina, A. Rebolledo, C. Cardozo, J.M. Toro, A. Durán, N. Labrador, E.M. Quintero, K. Imbachí, A. Delgado, M.B. Manrique, N. Murcia, A. Rojas-Triviño, M.L. Orozco, y D. Muñoz. 2013. Manual técnico: Tecnologías para el manejo de pitaya amarilla Selenicereus megalanthus (K. Schum. ex Vaupel) Moran en Colombia. Corporación Colombiana de Investigación Agropecuaria Corpoica, Palmira, COL.
Leslie, J., and B. Summerell. 2006. The Fusarium laboratory manual. 1st ed. Blackwell Publishing Ltd, Oxford, London, GBR.
Lops, F., F. Cibelli, M. L. Raimondo, and A. Carlucci. 2013. First report of stem wilt and root rot of Schlumbergera truncata caused by Fusarium oxysporum f. sp. Opuntiarum in Southern Italy. Plant Dis. 97:846-848. doi:10.1094/PDIS-11-12-1092-PDN
Mcdonald, J. 1993. Evolution and consequences of transposable elements. Curr. Opin. Genet. Dev. 3:855-864.
Murcia, N., A. Rojas, M. Orozco, N. Labrador, y J. Medina. 2013. Enfermedades limitantes en el cultivo de pitaya amarilla. En: T. Kondo et al., editores, Manual técnico: tecnología para el manejo de pitaya amarilla Selenicereus megalanthus (K. Schum. ex Vaupel) Moran en Colombia. Palmira, Corporación Colombiana de Investigación Agropecuaria Corpoica, Palmira, Valle del Cauca, COL. p. 78-87.
NCBI (National Center for Biotechnology Information). 2015. Nucleotide. http://blast.ncbi.nlm.nih.gov/Blast.cgi (accessed mar. 2015).
Nelson, P., M. Dignani, and E. Anaissie. 1994. Taxonomy, biology, and clinical aspects of Fusarium species. Clin. Microbiol. Rev. 7:479-504. doi:10.1111/j.1470-9465.2004.00845.x
O’Donnell, K., H. Kistler, E. Cigelnik, and R. Ploetz. 1998. Multiple evolutionary origins of the fungus causing Panama disease of banana: concordant evidence from nuclear and mitochondrial gene genealogies. Proc. Nal. Acad. Sci. 95:2044-2049.
O’Donnell, K., D. Sutton, A. Fothergill, D. Mccarthy, M. Rinaldi, and M. Brandt. 2008. Molecular phylogenetic diversity, multilocus haplotype nomenclature, and in vitro antifungal resistance within the Fusarium solani species complex. J. Clin. Microbiol. 46:2477-2490. doi:10.1128/JCM.02371-07
O’Donnell, K., D. Sutton, M. Rinaldi, K. Magnon, P. Cox, S. Revankar, S. Sanche, D. Geiser, J. juba, J. burik, A. Padhye, E. Anaissie, A. Francesconi, T. Walsh, and J. Robinson. 2004 Genetic diversity of human pathogenic members of the Fusarium oxysporum complex inferred from multilocus DNA sequence data and amplified fragment length polymorphism analyses: evidence for the recent dispersion of a geographically widespread clonal lineag. J. Clin. Microbiol. 42:5109-5120. doi:10.1128/JCM.42.11.5109-5120.
Pimentel, D., R. Zuñiga, and D. Morrison. 2005. Update on the environmental and economic cost associated with alien-invasive species in the United States. Ecol. Econ. 52:273-288
Rojas, A., A. Rebolledo, D. Kondo, J. Toro, y M. Orozco. 2008. Pitahaya amarilla (Selenicereus megalanthus). Plegable divulgativo. Centro de Investigación Palmira, Palmira, Valle del Cauca, COL.
Suárez, R. 2011. Evaluación de métodos de propagación en pitahaya amarilla Selenicereus megalanthus (Haw.) Britt & Rose y pitahaya roja Hylocereus polyrhizus (Haw.) Britt& Rose. Tesis MSc., Universidad Nacional de Colombia, Palmira, COL.
Tamura, K., G. Stecher, D. Peterson, A. Filipski, and S. Kumar. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725-2729. doi:10.1093/molbev/mst197
Valencia-Botín, A., and K. Hirotaka. 2013. A brief overview on pitahaya (Hylocereus spp.) diseases. Australas. Plant Pathol. 42:437-440. doi:10.1007/s13313-012-0193-8
Varón, F. 2006. Enfermedades de la pitahaya y su manejo. Rev. Asiava 73:19-21.
White, T., T. Bruns, S. Lee, and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: M.A. Innis et al., editors, PCR protocols. A guide to methods and applications. Academic Press, London, GBR. p. 315-322.
Wilson, K. 2001. Preparation of genomic DNA from bacteria. Current Protocols Mol. Biol. doi:10.1002/0471142727.mb0204s56
Wiwik, S., N. Dewa, S. Made, and I. Made. 2013. First report on Fusarium solani, a pathogenic fungus causing stem rot disease on dragon fruits (Hylocereus sp.) in Bali. J. Biol. Agric. Healthcare 3:93-99.
Wright, E.R., M.C. Rivera, A. Ghirlanda, and G.A. Lori. 2007. Basal rot of Hylocereus undatus caused by Fusarium oxysporum in Buenos Aires, Argentina. Plant Dis. 91:323-327. doi:10.1094/PDIS-91-3-0323A
Wulff, E.G., J.L. Sørensen, M. Lubeck, K.F. Nielsen, U. Thrane, and J. Torp. 2010. Fusarium spp. associated with rice Bakanae: ecology, genetic diversity, pathogenicity and toxigenicity. Environ. Microbiol. 12:649-657. doi:10.1111/j.1462-2920.2009.02105.x
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