In vitro test for the vegetative propagation of sugar cane (Saccharum officinarum L.) from young leaves.

Authors

  • José M. Cuellar Universidad Católica de Occidente (UNICO), Santa Ana, El Salvador.

DOI:

https://doi.org/10.15517/am.v8i1.24733

Abstract

This paper reports on the first in vitro cultivation tests done in El Salvador. The responses of cultivars MEX 70-485 and MEX 68-P-23 on various propagation stages, were evaluated in vitro. Young leaves still in the rolled stage were utilized. Explants 5 mm in lenght were placed on a Murashige and Skoog (1962) medium, modified for each stage of planttet recovery as follows: For the development and growth of “calluses” dosis, 2.0, 2.5 and 3.0 mg/l of 2.4/D were added and for differentiation, it was subtracted. To induce rhyzogenesis we used a medium with half of the macro-nutrients , one with the complete salts the other with the complete salts plus a supplement of 5 mg/l of AIA. Outstanding results were the following: In the phase of “calluses” formation, the major problem was the phenolization of the explants; the best 2.4-D acid concentration was 3.0 mgl, for both the calluses formation as for their growth and development. The differentiation was obtained with the renoval of 2.4-D acid from the medium, and the rhyzogenesis was favored with the supplement of 5 mg/l of AIA

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References

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VILLALOBOS R, I. M. 1986. Producción de somaclones en tres cultivos comerciales de caña de azúcar (Saccharum spp.) a partir de “callo”s inducidos in vitro Tesis Ing. Agr. Universidad de Costa Rica. C. R.

Published

2016-06-01

How to Cite

Cuellar, J. M. (2016). In vitro test for the vegetative propagation of sugar cane (Saccharum officinarum L.) from young leaves. Agronomía Mesoamericana, 8(1), 74–80. https://doi.org/10.15517/am.v8i1.24733