In vitro test for the vegetative propagation of sugar cane (Saccharum officinarum L.) from young leaves.
DOI:
https://doi.org/10.15517/am.v8i1.24733Abstract
This paper reports on the first in vitro cultivation tests done in El Salvador. The responses of cultivars MEX 70-485 and MEX 68-P-23 on various propagation stages, were evaluated in vitro. Young leaves still in the rolled stage were utilized. Explants 5 mm in lenght were placed on a Murashige and Skoog (1962) medium, modified for each stage of planttet recovery as follows: For the development and growth of “calluses” dosis, 2.0, 2.5 and 3.0 mg/l of 2.4/D were added and for differentiation, it was subtracted. To induce rhyzogenesis we used a medium with half of the macro-nutrients , one with the complete salts the other with the complete salts plus a supplement of 5 mg/l of AIA. Outstanding results were the following: In the phase of “calluses” formation, the major problem was the phenolization of the explants; the best 2.4-D acid concentration was 3.0 mgl, for both the calluses formation as for their growth and development. The differentiation was obtained with the renoval of 2.4-D acid from the medium, and the rhyzogenesis was favored with the supplement of 5 mg/l of AIA
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