In vitro test for the vegetative propagation of sugar cane (Saccharum officinarum L.) from young leaves.
DOI:
https://doi.org/10.15517/am.v8i1.24733Abstract
This paper reports on the first in vitro cultivation tests done in El Salvador. The responses of cultivars MEX 70-485 and MEX 68-P-23 on various propagation stages, were evaluated in vitro. Young leaves still in the rolled stage were utilized. Explants 5 mm in lenght were placed on a Murashige and Skoog (1962) medium, modified for each stage of planttet recovery as follows: For the development and growth of “calluses” dosis, 2.0, 2.5 and 3.0 mg/l of 2.4/D were added and for differentiation, it was subtracted. To induce rhyzogenesis we used a medium with half of the macro-nutrients , one with the complete salts the other with the complete salts plus a supplement of 5 mg/l of AIA. Outstanding results were the following: In the phase of “calluses” formation, the major problem was the phenolization of the explants; the best 2.4-D acid concentration was 3.0 mgl, for both the calluses formation as for their growth and development. The differentiation was obtained with the renoval of 2.4-D acid from the medium, and the rhyzogenesis was favored with the supplement of 5 mg/l of AIA
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CATALOGO DE VARIEDADES DE CAÑA DE AZUCAR. 1991. Compañía azucarera salvadoreña. Departamento agrícola, Centro experimental y capacitación. El Salvador. 63 p.
HEINZ, D.J. ; MEE, G. 1969. Plant differentiation from callus tissue of Saccharum species. Crop. Sci. 9:346-348.
MURASHIGE, T.; SKOOG, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:437-497.
PAYAN, F.C.; TARCON, G. 1977. Técnicas para la micropropagación de la caña de azúcar mediante el cultivo de tejido y de yemas axilares. Acta Agronómica. 27(1/4).
ROCA, M.W.; MROGINSKI, L.A. 1991. Cultivo de Tejidos en la Agricultura: Fundamentos y aplicaciones. CIAT (Centro Internaciol de Agricultura Tropical). Cali, Colombia. p. 542 -575.
THORPE, T. A. 1981. Plant tissue culture methods and applications in agriculture. Academic Press, Nueva York.
VILLALOBOS R, I. M. 1986. Producción de somaclones en tres cultivos comerciales de caña de azúcar (Saccharum spp.) a partir de “callo”s inducidos in vitro Tesis Ing. Agr. Universidad de Costa Rica. C. R.
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