ía Mesoamericana ISSN electrónico: 2215-3608

An alternative inoculation technique of Colletotrichum gloeosporioides on mango for early anthracnose tolerance screening

Abraham Monteon Ojeda, José Sergio Sandoval Islas, José Antonio Mora Aguilera, Carlos De Leon García De Alba, Amado Peréz Rodríguez, Alfonso Vasquez Lopez



The importance of having a technique that allows an efficient expression of symptoms of anthracnose is based on the early differentiation of cultivars and the optimization of genetic, material and financial resources. The objective of this research was to generate an alternative inoculation technique for Colletotrichum gloeosporioides on mango for early anthracnose tolerance screening. On this technique was optimized some of the most relevant components such as isolate virulence, conidial density, the inoculum deposition on leaves and using of surfactants. The study was carried in Iguala, Mexico, during the production cycles 2015-2016. C. gloeosporioides was biologically and culturally characterized. Gro and Sin monosporic strains were isolated from leaves, flowers, fruits and branches with anthracnose symptoms from commercial mango orchards located in Guerrero and Sinaloa states, Mexico. These strains show mycelial growth at 2.2 and 2.1 cm of diameter per day, spore density of 4.3x106, 3.9x106 conidia/ml, germination of 27 and 26%, virulence, with incubation period 4.5 and 4.1 days after inoculation, incidence of 90 and 92% and severity of 3.2 and 3.5 cm of diameter. The highest incidence and severity values with the lowest incubation period, was obtained using Gro isolate (1x105 conidia/ml) and polyoxyethylene-20-sorbitan monolaurate as spreader-sticker inoculated on the abaxial surface on detached young leaves, 15-20 days old, with a soft brush and incubated under dark condition. This inoculation technique allowed the optimal expression of C. gloeosporioides virulence in mango leaves and could be incorporated as a tool in the early differentiation of tolerance and susceptibility among cultivars.


conidial; spores; Mangifera indica; isolation techniques.

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Acosta, M., D. Nieto-Ángel, J.L. Domínguez-Álvarez, y J.L. Delgadillo-Sánchez. 2001. Calidad y tolerancia en frutos de papaya (Carica papaya L.) a la inoculación del hongo Colletotrichum gloeosporioides Penz., en postcosecha. Rev. Chapingo Ser. Hort. 7:119-130.

Ainsworth, G.C., F.K. Sparrow, and A.S. Sussman. 1973.The fungi. An advanced treatise. Academic Press Inc., NY, USA.

Alemu, K., A. Ayalew, and K. Weldetsadi. 2014. Evaluation of antifungal activity of botanicals for postharvest management of mango anthracnose (Colletotrichum gloeosporioides). Int. J. Life Sci. 8:1-6. doi:10.3126/ijls.v8i1.9957

Arauz, L.F. 2000. Mango anthracnose: economic impact and current options for integrated management. Plant Dis. 84:600-611. doi:10.1094/PDIS.2000.84.6.600

Barnett, H.L., and B.B. Hunter. 1998. Illustrated genera of imperfect fungi. 4th ed. APS Press, MN, USA.

Bailey, J.A., and M.J. Jeger. 1992. Colletotrichum: biology, pathology and control. CAB Internacional Wallingford, GBR.

Biggs, A.R., and S.S. Miller. 2001. Relative susceptibility of selected apple cultivars to Colletotrichum acutatum. Plant Dis. 85:657-660. doi:10.1094/PDIS.2001.85.6.657

Carreon, S.L., K.A. Balderas, M.A. Wong, D.R. Rosas, y E.G. Fentanes. 2010. Biofungicidas para el control de la antracnosis del mango: logrando frutos de alta calidad internacional para mercados exigentes. Claridades Agropecu. 20(208):28-37.

Denoyes-Rothan, B.G., C.D. Guérin, B. Smith, D. Minz, M. Maymon, and S. Freeman. 2003. Genetic diversity and pathogenic variability among isolates of Colletotrichum species from strawberry. Phytopathology 93:219-228. doi:10.1094/PHYTO.2003.93.2.219.

Doster, M.A., and W.C. Schnathorst. 1992. Comparative susceptibility of various grapevine cultivars to the powdery mildew fungus Uncinula necator. Am. J. Enol. Viticult. 36(2):101-104.

Espinosa, A.J., S.J. Arias, P.H. Rico, S.M. Miranda, y C.X. Chávez. 2004. Dinámica del daño y control de la antracnosis Colletotrichum gloeosporioides (Penz.) en mango de Michoacán. INIFAP, MEX.

Estrada-Valencia, M.N., P.E. Vélez-Arango, y E.C. Montoya-Restrepo. 1997. Caracterización de cultivos monospóricos del hongo Beauveria bassiana. CENICAFÉ 48(4):217-224. FAO. 2016. Food and agriculture data. FAO.*/E (accessed Jun. 24 2017).

Frias, G.A., L.H. Purdy, and R.A. Schmidt. 1995. An inoculation method for evaluating resistance of cacao to Crinipellis perniciosa. Plant Dis. 79:787-791. doi:10.1094/PD-79-0787

Gutiérrez-Alonso, O., J.G. Gutiérrez, D.A. Nieto, D.O. Téliz, E.M. Zavaleta, F.S. Delgadillo, y H.H. Vaquera. 2003. Evaluación de resistencia a benomil, thiabendazol y azoxystrobin para el control de antracnosis (Colletotrichum gloeosporioides Penz.) en frutos de guayaba (Psidium guajava L.) en postcosecha. Rev. Mex. Fitopatol. 21:228-232.

Gutiérrez-Alonso, J.G., D. Nieto-Ángel, D. Téliz-Ortiz, E. Zavaleta-Mejía, H. Vaquera-Huerta, T. Martínez-Damián, y F. Delgadillo-Sánchez. 2001. Características de crecimiento, germinación, esporulación y patogenicidad de aislamientos de Colletotrichum gloeosporioides Penz. obtenidos de frutos de mango (Mangifera indica L.). Rev. Mex. Fitopatol. 19:90-93.

Hernández. A., J. Pineda, A.G. Vera, E. Gil, H. Nass, y V. Barrientos. 2005. Técnica de inoculación rápida y eficiente para la evaluación de materiales de maíz ante “Rhizoctonia solani Kühn”. Bioagro 17:93-98.

Kuc, J., and S. Richmond. 1977. Aspects of the protection of cucumber against Colletotrichum lagenarium by Colletotrichum lagenarium. Phytopathology 67:533-536.

Montesinos-Matías, R., Viniegra-González, G., Alatorre-Rosas, R., and O. Loera. 2011. Relationship between virulence and enzymatic profiles in the cuticle of Tenebrio molitor by 2-deoxy-D-glucose-resistant mutants of Beauveria bassiana (Bals.) Vuill. World J. Microbiol. Biotechnol. 27:2095-2102. doi:10.1007/s11274-011-0672-z

Moral, J.K., R. De-Oliveira, and A. Trapero. 2009. Elucidation of the disease cycle of olive anthracnose caused by Colletotrichum acutatum. Phytopathology 99:548-556. doi:10.1094/PHYTO-99-5-0548.

Moral, J.K., and A. Trapero. 2009. Assessing the susceptibility of olive cultivars to anthracnose caused by Colletotrichum acutatum. Plant Dis. 93:1028-1036. doi:10.1094/PDIS-93-10-1028

Nelson, B., W.G. Lima, J.M. Tovar-Pedraza, S.J. Michereff, and M. Câmara. 2014. Comparative epidemiology of Colletotrichum species from mango in northeastern Brazil. Eur. J. Plant Pathol. 141:679-688. doi:10.1007/s10658-014-0570-y

Paéz, A.R. 1997. Respuesta de cultivares de mango (Mangifera indica L.) a la antracnosis en la Costa Atlántica colombiana. Rev. CORPOICA 2(1):45-53. doi:10.21930/rcta.vol2_num1_art:162

Ploetz, R.C., and O. Prakash. 2000. Foliar, floral and soilborne diseases. In: E.R. Litz, editor, The mango: botany, production and uses. CAB International, London, GBR. p. 281-285

Prusky, D. 1994. Mango-Anthracnosis. In: R.C. Ploetz et al., editors, Compendium of tropical fruit diseases. APS Press, St. Paul, MN, USA. p. 88-92

Prusky, D., I. Kobiler, I. Miyara, and N. Alkan. 2009. Fruit diseases. In: R.E. Litz, editor, 2nd ed. The mango: botany, production and uses, CAB International, Wallingford, GBR.

Rojas-Martínez, R.I., E. Zavaleta-Mejía, D. Nieto-Ángel, and M. Acosta-Ramos. 2008. Virulence and genetic variation of isolates of Colletotrichum gloeosporioides (Penz.) Penz. and Sacc. on mango (Mangifera indica L.) cv. Haden. Rev. Mex. Fitopatol. 26:21-26.

SAS Institute Inc. 2012. Base SAS® 9.3 Procedures guide: statistical procedures. 3th ed. SAS Institute Inc., Cary, NC, USA.

Sauceda-Acosta, C.P., G.A. Lugo-García, H.E. Villaseñor-Mir, L. Partida-Ruvalcaba, y Á. Reyes-Olivas. 2015. Un método preciso para medir severidad de roya de la hoja (Puccinia triticina Eriksson) en trigo. Rev. Fitotec. Mex. 38:427-434.

Sinha, P., R. Prajneshu, and A. Varma. 2002. Growth models for powdery mildew development of mango. Ann. Plant Prot. Sci. 10:84-87.

Stintzi, A., T. Heitza, V. Prasadb, S. Wiedemann-Merdinoglu, S. Kauffmanna, P. Geoffroya, M. Legranda, and B. Fritiga. 1993. Plant ‘pathogenesis-related’ proteins and their role in defense against pathogens. Biochimie 75:687-706. doi:10.1016/0300-9084(93)90100-7

Than, P.P., R. Jeewon, K.D. Hyde, S. Pongsupasamit, O. Mongkolporn, and P.W. Taylor. 2008. Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathol. 57:562-572. doi:10.1111/j.1365-3059.2007.01782.x

Tu, J.C. 1986. A detached leaf technique for screening beans (Phaseolus vulgaris L.) in vitro against anthracnose (Colletotrichum lindemuthianum). Can. J. Plant Sci. 66:805-809. doi:10.4141/cjps86-100

Willocquet, L., D. Colombet, M. Rougier, J. Fargues, and M. Clerjeau. 1996. Effects of radiation, especially ultraviolet B, on conidial germination and mycelial growth of grape powdery mildew. Eur. J. Plant Pathol. 102:441-449. doi:10.1007/BF01877138


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