Radiosensitivity of three tomato varieties (Solanum lycopersicum L.) irradiated with Cobalt-60 gamma rays
DOI:
https://doi.org/10.15517/am.2024.54329Keywords:
mutation, irradiation, genetic variability, mutagenic doseAbstract
Introduction. Tomato (Solanum lycopersicum L.) is considered one of the most important crops in the world. Genetic improvement of tomatoes has employed different methods to generate variability, and mutation the induction of mutations is one of the techniques used to obtaining improved varieties. The first step in using this tool is studying radiosensitivity to determine the irradiation dose to be used. Objective. To determine the radiosensitivity and the dose of cobalt-60 gamma rays to be used in three tomato varieties. Materials and methods. This research was conducted in January 2020 at the Instituto Nacional de Ciencias Agrícolas of Cuba. Seeds of three tomato varieties were irradiated with cobalt-60 gamma rays, with doses ranging from 100 Gy to 900 Gy, at intervals of 100 Gy; an unirradiated control was also used. The percentage of germination, plant height, and seedling survival were evaluated. Results. It was observed that higher irradiation dose reduced germination, survival, and plant height. The IDIAP T-7 and IDIAP T-9 varieties reduced germination at a dose of 400 Gy, while the DINA RPs cultivar reduced germination at 300 Gy. Genotypes showed survival values below 50 % at a dose of 800 Gy. Height reduction began at doses of 200 Gy for IDIAP T-7, 300 Gy for DINA RPs, and 400 Gy for IDIAP T-9. Conclusion. The DL-50 doses were identified as 692, 588, and 630 Gy, and the GR-50 doses were 407, 467, and 380 Gy for the IDIAP T-7, IDIAP T-9, and DINA RPs varieties, respectively. The GR-50 dose of each variety was selected for the mutation breeding program.
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Ahumada-Flores, S., Briceño-Zamora, M. F., García-Montoya, J. A., López-Cázarez, C., Pereo-Galvez, A. E., Parra-Cota, F. I., & de los Santos-Villalobos, S. (2020). Gamma radiosensitivity study on wheat (Triticum turgidum ssp. durum). Open Agriculture, 5(1), 558–562. https://doi.org/10.1515/opag-2020-0057
Ali Zafar, S., Aslam, M., Albaqami, M., Ashraf, A., Hassan, A., Iqbal, J., Maqbool, A., Naeem, M., Al-Yahyai, R., & Tan Kee Zuan, A. (2022). Gamma rays induced genetic variability in tomato (Solanum lycopersicum L.) germplasm. Saudi Journal of Biological Sciences, 29(5), 3300–3307. https://doi.org/10.1016/j.sjbs.2022.02.008
Brunner, H. (1995). Radiation induced mutations for plant selection. Applied Radiation and Isotopes, 46(6–7), 589–594. https://doi.org/10.1016/0969-8043(95)00096-8
Çelik, Ö., & Atak, Ç. (2017). Applications of ionizing radiation in mutation breeding. In A. M. Maghraby (Ed.), New Insights on Gamma Rays (pp. 111–132). InTech Open. https://doi.org/10.5772/66925
Datta, S. K. (2009). A report on 36 years of practical work on crop improvement through induced mutagenesis. In Q. Y. Shu (Ed.), Induced plant mutations in the genomics era (pp. 253–256). Food and Agriculture Organisation, & International Atomic Energy Agency. https://www.fao.org/3/i0956e/I0956e.pdf
Ernest, F. P, Noëlle, M. A. H., Godswill, N. -N., Thiruvengadam, M., Simon, O. A., Bille, N. H., Martin, B. J., Rebezov, M., & Shariati, M. A. (2020). Radiosensitivity of two varieties of watermelon (Citrullus lanatus) to different doses of gamma irradiation. Brazilian Journal of Botany, 43(4), 897–905. https://doi.org/10.1007/s40415-020-00659-8
Gruère, G. P., & Rosegrant, M. W. (2008). Assessing the implementation effects of the biosafety protocol’s proposed stringent information requirements for genetically modified commodities in countries of the Asia pacific economic cooperation. Review of Agricultural Economics, 30(2), 214–232. https://onlinelibrary.wiley.com/doi/10.1111/j.1467-9353.2008.00401.x
Guerra Murillo, J. A, Villarreal Nuñez, J. E., Herrera Vásquez, J. A., Aguilera Cogley, V., & Osorio Burgos, O. (2016). Manejo integrado del cultivo de tomate industrial (Manual técnico). Instituto de Investigación Agropecuaria de Panamá. http://www.idiap.gob.pa/download/manual-tecnico-manejo-integrado-del-cultivo-de-tomate-industrial/?wpdmdl=3309
Hazra, S., Gorai, S., Bhattacharya, S., Bose, S., Hazra, P., Chattopadhyay, A., & Maji, A. (2022). Radio-sensitivity of diverse tomato genotypes with respect to optimization of gamma irradiation dose. Brazilian Journal of Botany, 45, 917–927. https://doi.org/10.1007/s40415-022-00823-2
Hazra, S., Gorai, S., Umesh Kumar, V., Bhattacharya, S., Maji, A., Jambhulkar, S., Ali, N., & Chattopadhyay, A. (2021). Optimization of gamma radiation dose for induction of mutations in okra. International Journal of Vegetable Science, 27(6), 574–584. https://doi.org/10.1080/19315260.2021.1894626
Hernández-Jiménez, A., Pérez-Jiménez, J. M., Bosch-Infante, D., & Speck, N. C. (2019). La clasificación de suelos de Cuba: énfasis en la versión de 2015. Cultivos Tropicales, 40(1), a15–e15. https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1504/2622
Hernández-Muñoz, S., Pedraza-Santos, M. E., López, P. A., De la Cruz-Torres, E., Fernández-Pavía, S. P., Martínez-Palacios, A., & Martínez-Trujillo, M. (2017). Determinación de la DL50 Y GR 50 con rayos gamma (60Co) en protocormos de Laelia autumnalis in vitro. Agrociencia, 51(5), 507–524. https://agrociencia-colpos.org/index.php/agrociencia/article/view/1307/1307
Iglesias-Andreu, L. G., Sánchez-Velásquez, L. R., Tivo-Fernández, Y., Luna-Rodríguez, M., Flores-Estévez, N., Noa-Carrazana, J. C., Ruiz-Bello, C., & Moreno-Martínez, J. L. (2010). Efecto de radiaciones gamma en Abies religiosa (Kunth) Schltd. et Cham. Revista Chapingo Serie Ciencias Forestales y del Ambiente, 16(1), 5–12.
Jaipo, N., Kosiwikul, M., Panpuang, N., & Prakrajang, K. (2019). Low dose gamma radiation effects on seed germination and seedling growth of cucumber and okra. Journal of physics: Conference series, 1380, Article 012106. https://doi.org/10.1088/1742-6596/1380/1/012106
Just, D., Garcia, V., Fernandez, L., Bres, C., Mauxion, J.-P., Petit, J., Jorly, J., Assali, J., Bournonville, C., Ferrand, C., Baldet, P., Lemaire-Chamley, M., Mori, K., Okabe, Y., Ariizumi, T., Asamizu, E., Ezura, H., & Rothan, C. (2013). Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato. Plant Biotechnology, 30(3), 225–231. https://doi.org/10.5511/plantbiotechnology.13.0622a
Kon, E., Ahmed, O. H., Saamin, S., & Majid, N. M. A. (2007). Gamma radiosensitivity study on long bean (Vigna sesquipedalis). American Journal of Applied Sciences, 4(12), 1090–1093.
Laskar, R. A., & Khan, S. (2014). Mutagenic effects of MH and MMS on induction of variability in broad bean (Vicia faba L.). Annual Research & Review in Biology, 4(7), 1129–1140. https://doi.org/10.9734/arrb/2014/6553
Madriz-Martínez, M., Fernández-Acuña, A., Hernandez-Villalobos, S., Orozco-Rodríguez, R., & Argüello-Delgado, J. (2022). Radiosensibilidad del arroz (Oryza sativa L. var CR5272) por irradiación gamma en Costa Rica. Cultivos tropicales, 43(1), Artículo 08. https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1644/3233
Manova, V., & Gruszka, D. (2015). DNA damage and repair in plants - from models to crops. Frontiers in Plant Science, 6, Article 885. https://doi.org/10.3389/fpls.2015.00885
Mudibu, J., Nkongolo, K. K. C., Kalonji-Mbuyi, A., & Kizungu, R. V. (2012). Effect of gamma irradiation on Morpho-agronomic characteristics of soybeans (Glycine max L.). American Journal of Plant Sciences, 3(3), 331–337. https://doi.org/10.4236/ajps.2012.33039
Murti, R. H., Kim, H. Y., & Yeoung, Y. R. (2013). Effectiveness of gamma ray irradiation and ethyl methane sulphonate on in vitro mutagenesis of strawberry. African Journal of Biotechnology, 12(30), 4803–4812. https://doi.org/10.5897/ajb12.1386
Norfadzrin, F., Ahmed, O. H., Shaharudin, S., & Abdul Rahman, D. (2007). A preliminary study on gamma radiosensitivity of tomato (Lycopersicon esculentum) and okra (Abelmoschus esculentus). International Journal of Agricultural Research, 2(7), 620–625. https://doi.org/10.3923/ijar.2007.620.625
Patil, U. H., Karale, A. R., Katwate, S. M., & Patil, M. S. (2017). Mutation breeding in chrysanthemum (Dendranthema grandiflora T.). Journal of Pharmacognosy and Phytochemistry, 6(6), 230–232. https://www.phytojournal.com/archives?year=2017&vol=6&issue=6&ArticleId=2083
Pavan Kumar, D., & Venu-Babu, P. (2011). Gamma radiosensitivity study on rice (Oryza sativa L.). Asian Journal of Plant Science & Research, 3(1), 54–68.
Peng, C. S., Hoe, P. C. K., Bahari, N., & Shamsudin, S. (2020). Radiosensitivity test of tomato (Solanum lycopersicum) to gamma irradiation. Jurnal Sains Nuklear Malaysia, 32(1), 1–9. https://202.186.20.70/index.php/jsnm/article/view/159
Postelnicu, T. (2011). Probit analysis. In M. Lovric (Ed.), International Encyclopedia of Statistical Science (pp. 1128–1131). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-04898-2_461
Ramírez, R., González, L. M., Camejo, Y., Zaldívar, N., & Fernández, Y. (2006). Estudio de radiosensibilidad y selección del rango de dosis estimulantes de rayos X en cuatro variedades de tomate (Lycopersicon esculentum Mill). Cultivos Tropicales, 27(1), 63–67. https://ediciones.inca.edu.cu/index.php/ediciones/article/view/398/pdf
Ranjan, A., Ichihashi, Y., & Sinha, N. R. (2012). The tomato genome: implications for plant breeding, genomics and evolution. Genome Biology, 13(8), Article 167. https://doi.org/10.1186/gb-2012-13-8-167
Roslim, D. I., Herman, & Fiatin, I. (2015). Lethal dose 50 (ld 50) of mungbean (Vigna radiata L. Wilczek) cultivar kampar. SABRAO Journal of Breeding & Genetics, 47(4), 510–516. http://sabraojournal.org/wp-content/uploads/2018/01/SABRAO-J-Breed-Genet-47-4-510-516-Dewi.pdf
Saraswathi, M. S., Kannan, G., Uma, S., Thangavelu, R., & Backiyarani, S. (2016). Improvement of banana cv. Rasthali (Silk, AAB) against Fusarium oxysporum f.sp. cubense (VCG 0124/5) through induced mutagenesis: Determination of LD50 specific to mutagen, explants, toxins and in vitro and in vivo screening for Fusarium wilt resistance. Indian Journal of Experimental Biology, 54(5), 345–353.
Shu, Q. Y., Forster, B. P., & Nakagawa, H. (2012). Principles and applications of plant mutation breeding. In Q. Y. Shu, B. P. Forster, & H. Nakagawa. (Eds.), Plant mutation breeding and biotechnology (pp. 301–325). CABI. https://doi.org/10.1079/9781780640853.0301
Sikder, S., Biswas, P., Hazra, P., Akhtar, S., Chattopadhyay, A., Badigannavar, A. M., & D’Souza, S. F. (2013). Induction of mutation in tomato (Solanum lycopersicum L.) by gamma irradiation and EMS. The Indian Journal of Genetics & Plant Breeding, 73(4), 392–399. https://doi.org/10.5958/j.0975-6906.73.4.059
Spencer-Lopes, M. M., Forster, B. P., & Jankuloski, L. (Eds.) (2021). Manual de mejoramiento por mutaciones (3a ed.). Organización de las Naciones Unidas para la Alimentación y la Agricultura, & Organismo Internacional de Energía Atómica. https://www.fao.org/3/i9285es/I9285ES.pdf
Trivedi, M., Singh, R., Shukla, M., & Tiwari, R. K. (2016). GMO and food security. In Omkar (Ed.), Ecofriendly pest management for food security (Chapter 23, pp. 703–726). Elsevier. https://doi.org/10.1016/b978-0-12-803265-7.00023-3
Villaseñor Mir, H. E. (2018). Sistema de mejoramiento genético de trigo en México. Revista Mexicana de Ciencias Agrícolas, (11), 2183–2189. https://doi.org/10.29312/remexca.v0i11.796
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Copyright (c) 2024 Jorge Enrique Jaén Villarreal, María Caridad González Cepero, Ismael Camargo Buitrago, Ana Elida Sáez Cigarruista, Rodolfo Guillama Alonso, José Ángel Guerra Murillo
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