Ovulation induction in cows with Kisspeptin-10 in an estrus synchronization protocol

Authors

DOI:

https://doi.org/10.15517/am.2024.55231

Keywords:

GnRH, induced ovulation, peptides, subtropical climate, cows

Abstract

Introduction. In prepubertal heifers, Kisspeptin-10 has stimulated the secretion of luteinizing hormone (LH) and follicle-stimulating hormone (FSH), and in postpartum lactating cows, it has stimulated LH secretion without evidence of inducing ovulation. Objective. To induce ovulation using Kisspeptin-10 or gonadotropin-releasing hormone (GnRH) in beef-producing cows under subtropical conditions in Mexico. Materials and methods. The study was conducted in the northeastern region of the state of Puebla uring the years 2020 to 2021, with 252 Bos taurus x Bos indicus cows. The estrous synchronization protocol involved the insertion of an intravaginal device impregnated with progesterone (1.9 g) and intramuscular injection of 2 mg of estradiol benzoate (day 0). On day seven, the intravaginal device was removed, and cows were immediately intramuscularly injected with 0.150 mg of D-cloprostenol. On the following day (day eight), Kisspeptin-10 was as effective as GnRH in inducing ovulation. 56 hours after the removal of the intravaginal device (day nine), cows were intramuscularly injected with GnRH or Kisspeptin-10. Cows were assigned to four treatments: T1, 100 μg of GnRH (positive control), T2, T3, and T4 with 500 μg, 1000 μg, and 1500 μg of Kisspeptin-10, respectively. Ovulation was detected via ultrasonography four days post-treatment. The response variable evaluated was the ovulation rate. Data were analyzed using generalized linear models with a logit link function. Results. Ovulation rates obtained with T1 and T4 were statistically equal (99.9%; p>0.05) and higher (p<0.05) than those achieved with T2 (98.5%) and T3 (88.4%), with the latter two being statistically equal (p>0.05). Conclusion. Kisspeptin-10 1500 μg was as effective as GnRH in inducing ovulation.

Downloads

Download data is not yet available.

References

Alamilla Rodríguez, M., Calderón Robles, R. C., Rosete Fernández, J. V., Rodríguez Hernández, K., Vera Ávila, H. R., Arreguín Arévalo, J. A., Nett, T. M., Gutiérrez Aguilar, C. G., González Padilla, E., Gómez-Chavarín, M., & Villa Godoy, A. (2017). Kisspeptina en becerras prepúberes: I. Influencia de la edad en la respuesta de LH, FSH y GH a kisspeptina-10 y su asociación con IGF-I, leptina y estradiol. Revista Mexicana de Ciencias Pecuarias, 8(4), 375–385. https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/4644/3772

Báez, S. G., & Grajales, L. H. (2009). Anestro posparto en ganado bovino en el trópico. Revista MVZ Córdoba, 14(3), 1867–1875. https://revistamvz.unicordoba.edu.co/article/view/347/415

Guzmán Sánchez, A., Rosales-Torres, A. M., & Gutiérrez Aguilar, C. G. (2012). Neuroendocrine effects of insulin, IGF-I and leptin on the secretion of the gonadotropin-releasing hormone (GnRH). Tropical and Subtropical Agroecosystems, 15(Sup 1), S79–S90. https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/1277/646

Islam, R. (2011). Synchronization of estrus in cattle: A review. Veterinary World, 4(3), 136–141. https://doi.org/10.5455/vetworld.2011.136-141

Macedo, G. G., Mingoti, R. D., Batista, E. O. S., Monteiro, B. M., Vieira, L. M., Barletta, R. V., Wiltbank, M. C., Nogueira, G. P., Rennó, F. P., Maio, J. R., & Baruselli, P. S. (2019). Profile of LH release in response to intramuscular treatment with kisspeptin in Bos indicus and Bos taurus prepuberal heifers. Theriogenology, 125, 64–70. https://doi.org/10.1016/j.theriogenology.2018.10.011

Pinilla, L., Aguilar, E., Dieguez, C., Millar, R. P., & Tena-Sempere, M. (2012). Kisspeptins and reproduction: physiological roles and regulatory mechanisms. Physiological Reviews, 92, 1235–1316. https://journals.physiology.org/doi/epdf/10.1152/physrev.00037.2010

Roa, N., Linares, T., D´Enjoy, D´Endel, Marín, C., & Martínez, N. (2012). Sincronización del ciclo estral con GnRH y PGF2alfa en vacas con tres tipos morfométricos diferentes, en el Llano Venezolano. Revista Electrónica de Veterinaria, 13(11), 1–8.

Rosete, F. J. V., Hernández, B. L., Santos, E. R., Gómez-Chavarín, B. M., Perera, M. G., Calderón, R. R. C., Vera, A. H. R., Villagómez, A. M. E., & Villa-Godoy, A. (2015). Respuesta de hormona luteinizante a aplicaciones repetidas de kisspeptina-10 en vacas en anestro posparto. En R. Basurto Gutiérrez, & A. M. Anaya Escalera (Eds.), LI Reunión Nacional de Investigación Pecuaria (Vol. 1, pp. 61). Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias.

Santos, E. R., Calderón, R. R. C., Rosete, F. J. V., Perera, M. G., Murcia, M. C., Vera, A. H. R., Gutiérrez, A. C. G., Arreguín, A. J. A., & Villa-Godoy, A. (2012). Efecto de la kisspeptina (KISS-10) administrada en forma repetida en la liberación de la LH inducción de la ovulación y su asociación con la composición corporal y el IGF-1 sérico en becerras prepúberes. En R. Basurto Gutiérrez, A. M. Anaya Escalera, & M. D. Montoya Flores (Eds.), XLVIII Reunión Nacional de Investigación Pecuaria (pp. 102). Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias.

Santos, E. R., Calderón, R. R.C., Rosete, F. J. V., Perera, M. G., Murcia, M. C., Villagómez, A. M. E., Cárdenas, L. M., Vera, A. H. R., Gutiérrez, A. C. G., Arreguín, A. J. A., & Villa-Godoy, A. (2012). Evaluación de la sensibilidad del eje gonadotrópico a dosis bajas de kisspeptina (KISS-10) en becerras prepúberes [Resumen]. En R. Basurto Gutiérrez, A. M. Anaya Escalera, & M. D. Montoya Flores (Eds.), XLVIII Reunión Nacional de Investigación Pecuaria (pp. 125). Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias.

Santos Echeverría, R., Calderón Robles, R. C., Vera Ávila, H. R., Perera-Marín, G., Arreguín Arévalo, J. A., Nett, T. M., Gutiérrez Aguilar, C. G., & Villa-Godoy, A. (2014). Hormona luteinizante y actividad ovárica en respuesta a kisspeptina-10 y su asociación con IGF-1 y leptina en becerras pre-púberes. Revista Mexicana de Ciencias Pecuarias, 5(2), 181–200. https://cienciaspecuarias.inifap.gob.mx/index.php/Pecuarias/article/view/3224

Villa Godoy, A., Santos Echeverría, R., Rosete Fernández, J. V., Calderón Robles, R. C., Perera Marín, G., Arreguín Arévalo, J. A., & Nett, T. M. (2018). Kisspeptina en becerras prepúberes: 2. Respuesta de LH, FSH y GH a distintas dosis de kisspeptina-10 y su asociación con IGF-I y leptina circulantes. Revista Mexicana de Ciencias Pecuarias, 9(4), 719–737. https://doi.org/10.22319/rmcp.v9i4.4192

Whitlock, B. K., Daniel, J. A., Wilborn, R. R., Rodning, S. P., Maxwell, H. S., Steele, B. P., & Sartin, J. L. (2008). Interaction of estrogen and progesterone on Kisspeptin-10-stimulated luteinizing hormone and growth hormone in ovariectomized cows. Neuroendocrinology, 88(3), 212–215. https://doi.org/10.1159/000146242

Whitlock, B. K., Daniel, J. A., Wilborn, R. R., Maxwell, H. S., Steele, B. P., & Sartin, J. L. (2010). Interaction of kisspeptin and somatotropic axis. Neuroendocrinology, 92(3), 178–188. http://doi.org/10.1159/000318049

Whitlock, B. K., Daniel, J. A., Wilborn, R. R., Maxwell, H. S., Steele, B. P., & Sartin, J. L. (2011). Effect of kisspeptin on regulation of growth hormone and luteinizing hormone in lactating dairy cows. Journal of Animal Science and Biotechnology, 2(3), 131–140. https://trace.tennessee.edu/utk_animpubs/4

Wiltbank, M. C., Gümen, A., & Sartori, R. (2002). Physiological classification of anovulatory conditions in cattle. Theriogenology, 57(1), 21–52. http://doi.org/10.1016/s0093-691x(01)00656-2

Yavas, Y., & Walton, J. S. (2000). Postpartum acyclicity in suckled beef cows. Theriogenology, 54(1), 25–55. http://doi.org/10.1016/S0093-691X(00)00323-X

Published

2024-01-09

How to Cite

Rosete Fernández, J. V., Fragoso Islas, A., Santos Echeverría, R., & Ríos Utrera, Ángel. (2024). Ovulation induction in cows with Kisspeptin-10 in an estrus synchronization protocol. Agronomía Mesoamericana, 35, 55231. https://doi.org/10.15517/am.2024.55231