Detección de crinivirus y begomovirus en plántulas de tomate y arvenses asociadas a semilleros
DOI:
https://doi.org/10.15517/ma.v28i2.25860Palabras clave:
Tomato chlorosis virus (ToCV), geminivirus, Solanum lycopersicum, moscas blancas.Resumen
El objetivo de este trabajo fue detectar infecciones causadas por el Tomato chlorosis virus (ToCV) y begomovirus en plántulas de tomate, y en las arvenses de los alrededores de los invernaderos. En el período de un año, a partir de abril de 2008, se recolectaron 168 muestras de tejido foliar, 90 de plántulas de tomate y 78 de arvenses en tres distintos semilleros en la provincia de Cartago, Costa Rica. Por medio de una transcripción reversa ligada a una reacción en cadena de la polimerasa en tiempo real (qRT-PCR), se determinaron infecciones con ToCV en un 18,9% de plántulas de tomate y en un 7,7% de las arvenses. Los begomovirus se detectaron por medio de hibridación con sonda de ADN no radioactiva. Los resultados de hibridación se con rmaron con una amplificación por el círculo rodante (RCA), seguida de una reacción en cadena de la polimerasa (PCR), utilizando dos pares de iniciadores degenerados. Ninguna muestra de tomate resultó positiva para los virus estudiados, mientras que seis arvenses estaban infectadas; dos de ellas Phytolacca icosandra y Brassica sp., mostraron coinfección con el ToCV. Los resultados sugieren que si las plántulas de tomate infectadas con ToCV se comercializan, este podría ser introducido en otras regiones del país. Las arvenses alrededor de los invernaderos mostraron ser potenciales fuentes de inóculo tanto del ToCV como de begomovirus.
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