Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

Heavy metal accumulation and biochemical evaluation of earthworms from sawmills in Abeokuta, South-Western Nigeria


stress enzymes
heavy metals
enzimas de estrés
metales pesados
lombrices de tierra

How to Cite

Bamidele, J. A., Idowu, A. B., Ademolu, K. O., Akinloye, O. A., & Bamgbola, A. A. (2015). Heavy metal accumulation and biochemical evaluation of earthworms from sawmills in Abeokuta, South-Western Nigeria. Revista De Biología Tropical, 63(4), 1213–1221.


Over the years, sawmilling industries have shown a high growth in the rain forest areas of Nigeria, releasing several wastes into the environment. This study aims at using earthworms (Eudrilus eugeniae, Libyodrilus violaceous and Hyperiodrilus africanus) of sawmill origin as bio-indicators of metal pollution in sawmills. Four major sawmills located in Abeokuta (7o9’11’’44” N - 3o19’35’’ E), namely Lafenwa, Sapon, Isale-Ake and Kotopo sawmills were used for this study. The arboretum of the Federal University of Agriculture, Abeokuta was used as control site. Earthworms, plant and soil samples were collected each month for three months (March to May, 2013), randomly from different points at each of the locations. Protein analysis was conducted on the earthworms using gel electrophoresis while the activities of Superoxide dismutase (SOD), Catalase (CAT) and Glutathione peroxidase (GPx) were done spectrophotometrically. Heavy metal analysis was also conducted on soil, plant and earthworm samples using Atomic Absorption Spectrophotometer. Gel electrophoresis results revealed the presence of nine protein bands in E. eugeniae from Sapon and Kotopo sawmills, as compared to six protein bands of E. eugeniae from the control site. Seven protein bands were observed in L. violaceous from Lafenwa and H. africanus from Isale-Ake sawmills. Levels of SOD, GPx and CAT activities were significantly higher (P < 0.05) in E. eugeniae from Sapon sawmill than those of Kotopo sawmill and the control site. This study also revealed that Pb and Cd concentrations were higher in the earthworms, plants and soil from the sawmills than those of the control site. Sapon sawmill recorded significantly higher (P < 0.05) levels of Cd and Cu in plants as well as Pb and Cd in soil samples than those of the other locations. The concentrations of Cu, Co and Ni were higher in the soil of the control site than in the sawmill soils. Stronger relationship exists in the metal concentrations between the earthworms and soils (R= 0.602) than between the plants and soil (R= 0.405). Sawmilling therefore posses potential risks on sawmill soil and soil fauna, especially earthworm species.


Abulude, F. O. (2006). Analysis of suspended air particulates along four sawmills in Nigeria during the wet and dry seasons. Journal of Engineering and Applied Sciences, 1(3), 224-226.

Agbaire, P. O., & Emoyan, O. O. (2012). Bioaccumulation of heavy metals by earthworm (Lumbricus terrestris) and associated soils in domestic dumpsite in Abraka, Delta state, Nigeria. International Journal of Plant, Animal and Environmental Sciences, 2(3), 204-209.

Association of Official Analytical Chemists (A.O.A.C.). (2006). Official method of analysis of the AOAC, 23rd Edition. Washington, DC.

Awofolu, O. R. (2005). A survey of trace metals in vegetation, soil and lower animals along some selected major roads in the metropolitan city of Lagos. Environmental Monitoring and Assessment, 105, 431-447.

Bamidele, J. A., Idowu, A. B., Ademolu, K. O., & Atayese, A. O. (2014). Microbial diversity and digestive enzyme activities in the gut of earthworms found in sawmill industries in Abeokuta, Nigeria. Revista de Biologia Tropical, 62(3), 1241-1249.

Booth, L. H., Heppelthwaite, V., & McGlinchy, A., (2000). The effect of environmental parameters on growth, cholinesterase activity and glutathione S-transferase activity in the earthworm (Aporrectodea caliginosa). Biomarkers, 5, 46-55.

Damien, C., Chantal, V. H., Pirouz, S., Zerimech, F. H., Laurence, J., & Jean, M. H. (2004). Cellular impact of metal trace elements in terricolous lichen Diploschistes muscorum (Scop.) R. Sant. -identification of oxidative stress biomarkers. Water Air and Soil Pollution, 152, 55-69.

Dosunmu, O. O., & Ajayi, A. B. (2002). Problems and management of sawmill waste in Lagos. Proceedings of International Environmental Pollution Control and Waste Management (EPCOWN 2002), pp. 271-278.

Elaigwu, S. E., Ajibola, V. O., & Folaranmi, F. M. (2007). Earthworm (Eudrilus eugenia Kinberg) as Bio-indicator of the Heavy Metal Pollution in Two Minicipal Dump Sites of Two Cities in Northern Nigeria. Research Journal of Environmental Sciences, 1(5), 244-250.

Haimi, J. (2000). Decomposer animals and bioremediation of soils. Environmental Pollution, 107, 233-238.

Hamdan, I. A., & Magdy, M. Y. (2010). Polyacrylamide gel electrophoresis of proteins extracted from Nematotaenia dispar which isolated from Varanus griseus in Saudi Arabia. Research Journal of Biological Sciences, 5(11), 735-738.

Hobbelen, P. H. F., Koolhaas, J. E., & Gestel, C. A. M. (2006). Bioaccumulation of heavy metals in earthworms Lumbricus rubellus and Aporrectodea caliginosa in relation to total and available metal concentrations in field soils. Environmental Pollution, 144, 639-646.

Holmgren, G. G. S., Meyer, R. L. C., & Daniels, R. B. (1993). Cadmium, Lead, Zinc, Copper, and Nickel in agricultural Soils of the United States of America. Journal of Environmental Quality, 22, 335-348.

Hotchkin, P. G., & Kaya, H. K. (1984). Electrophoresis of soluble proteins from two species of Xemrhdus, bacteria mutualistically associated with nematodes Steinernemu spp. and Neteromtis spp. Journal of General Microbiology, 130, 2725-273.

Idowu, A. B., Wewe, N., & Amusan, A. A. S. (2007). Heavy metal content of the variegated grasshopper Zonocerus variegatus (L.) (Orthoptera: Pygomorphidae) collected from various locations in Abeokuta, Ogun state, Nigeria. Nigerian Journal of Entomology, 24, 35-41.

Ihekwaba, A. E., Nwafor, A., & Adienbo, O. M. (2009). Lung Function Indices in Primary and Secondary Sawmill Workers in Port Harcourt, Nigeria. African Journal of Applied Zoology & Environmental Biology, 11, 101-105.

Kordarfshari, S., Hosseine, S. H., Meshgi, B., & Youssefi, M. R. (2010). Comparison of electrophoretic patterns of larval stages of taenidae and determination of specific antigens of hydatid cyst by western blotting technique. Global Veterinarian, 4, 601-606.

Lennox, J. A., Abriba, C., Alabi, B. N., & Akubuenyi, F. C. (2010). Comparative degradation of sawdust by microorganisms isolated from it. African Journal of Microbiology Research, 4(13), 1352-1355.

Lijun, L., Xuemei, L., Yaping, G., & Enbo, M. (2005). Activity of the enzymes of the antioxodative system in cadmium-treated Oxya chinensis (Orthoptera: Acridoidae). Environmental Toxicology and Pharmacology, 20, 412-416.

Misra, H. P., & Fridovich, I. (1972). The role of superoxide anion in the auto oxidation of epinephrine and simple assay for SOD. Journal of Biological Chemistry, 247(10), 3170-3175.

Nwajei, G. E., & Iwegbue, C. M. (2007). Trace elements in sawdust particles in the vicinity of sawmill in Sapele, Nigeria. Parkistan Journal of Biological Sciences, 10(23), 4311-4314.

Olayinka, O. T., Idowu, A. B., Dedeke, G. A., Akinloye, O. A., Ademolu, K. O., & Bamgbola A. A. (2011). Earthworm as Bio-indicator of Heavy Metal Pollution around Lafarge, Wapco Cement Factory, Ewekoro, Nigeria. Proceedings of the Environmental Management Conference, Federal University of Agriculture, Abeokuta, Nigeria, pp. 489-496.

Owa, S. O., Dedeke, G. A., & Yeye, A. J. (2002). Earthworm cast characteristics under Bahama grass and the question of why earthworms cast. African Journal of Science and Technology, 3(1-2), 33-35.

Owa, S. O., Olowoparija, S. B., Aladesida, A., & Dedeke, G. A. (2013). Enteric bacteria and fungi of the Eudrilid earthworm Libyodrilus violaceus. African Journal of Agricultural Research, 8(17), 1760-1766.

Rao, J. V., Surya, P., Y., & Madhavendra, S. S. (2003). Toxic effects of chlorpyrifoson survival, morphology and acetylcholinesterase activity of the earthworm Eisenia foetida. Ecotoxicology and Environmental Safety, 54, 296-301.

Reinecke, S. A., & Reinecke, A. J., (2004). The comet assay as biomarker of heavy metal genotoxicity in earthworms. Archives of Environmental Contamination and Toxicology, 46, 208-215.

Renu, B., Pandey H., Bisht,S. P., Kandpal, B., & Kaushal, B. R. (2006). Feeding and casting activities of the earthworm (Octolasion tyrtaeum) and their effects on crop growth under laboratory conditions. Tropical Ecology, 47(2), 291-294.

Ricketts, H. J., Morgan, A. J., Spurgeon, D. J., & Kille, P. (2004). Measurement of annetocin gene expression: a new reproductive biomarker in earthworm ecotoxicology. Ecotoxicology and Environmental Safety, 57, 4-10.

Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G., & Hoekstra, W. G. (1973). Selenium: Biochemical role as a component of Glutathione peroxidise. Science, 179(4073), 588-590.

Sinha, K. A. (1972). Colorimetric assay of catalase. Analytical Biochemistry, 47, 389-394.

Snider, R. D. (1973). Electrophoresis and the taxonomy of phytopathogenic fungi. Bulletin of the Torrey Botanical Club, 100, 272-276.

Sosorova, S. B., Merkusheva, M. G., Gyninova, A. B., & Ubugunov, L. L. (2012). The microelement composition of the soil-plant cover in the Basin of Lake Kotokel. Earth Science Research, 1(2), 229-239.

Spiegel, H. (2002). Trace element accumulation in selected bioindicators exposed to emissions along the industrial facilities of Danube Lowland. Turkish Journal of Zoology, 26, 815-823.

Spurgeon, D. J., & Hopkin, S. P. (1999). Tolerance to zinc in populations of the earthworm Lumbricus rubellus from uncontaminated and metal contaminated ecosystems. Archives of Environmental Contamination and Toxicology, 37, 332-337.

United States Department of Agriculture (USDA), (2000). Heavy Metal Soil Contamination. Soil Quality - Urban Technical Note No. 3, September, 2000.



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