Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://revistas.ucr.ac.cr/index.php/rbt/oai
Biochemical changes in Araucaria angustifolia (Araucariaceae) zygotic embryos during the storage
PDF
HTML

Keywords

Piño Brasileño
semillas recalcitrantes
deterioro de las semillas
estrés oxidativo
superóxido dismutasa.
Brazilian pine
recalcitrant seeds
seed deterioration
oxidative stress
superoxide dismutase.

How to Cite

Garcia, C., Medeiros Coelho, C. M., Maraschin, M., Farias Soares, F. L., Guerra, M. P., & Wilhelm-Filho, D. (2015). Biochemical changes in Araucaria angustifolia (Araucariaceae) zygotic embryos during the storage. Revista De Biología Tropical, 63(4), 1185–1196. https://doi.org/10.15517/rbt.v63i4.16415

Abstract

Reactive oxygen species (ROS) are present in all aerobic organisms, but the seed deterioration processes can increase its production. Oxidative stress caused by higher endogenous ROS may cause irreparable damage to cells, leading to the loss of seed viability. Considering that the enzyme superoxide dismutase (SOD) composes the first enzymatic antioxidant defense mechanism, this study aimed to evaluate zygotic embryos of Araucaria angustifolia recalcitrant seeds during storage regarding changes in SOD activity. Besides, some of the major alterations resulting from oxidative stress, e.g., lipid peroxidation and changes in proteins and DNA integrity were also measured. Approximately, 7 000 seeds were harvested from a population in Southern Brazil and stored for 180 days under laboratory (L, laboratory temperature), refrigeration (R, 5 ± 1 °C) and freezing (F, -18 ± 1 °C) conditions. The analysis of lipid peroxidation through TBARS levels, SOD activity, protein profile by electrophoretic separation, and integrity of genomic DNA were performed at 0, 60, 120, and 180 days of storage. The results revealed an increase in lipid peroxidation and SOD activity, especially during the L storage, a condition in which there was an extensive degradation of proteins. Some proteins (i.e., 45, 32, and 31 kDa) were expressed only in embryos stored under R and F conditions. No damage was observed in the nuclear DNA integrity for the evaluated period of seed storage at R and F conditions. The F samples maintained the biochemical traits of interest throughout the storage period, with the exception of the protein profile. However, such changes are limiting for the maintenance of seed viability. In conclusion, R storage can be indicated to delay the metabolic feature alterations that occur when seeds are exposed to conditions of natural ambient after harvest, extending the conservation period of A. angustifolia seeds.

https://doi.org/10.15517/rbt.v63i4.16415
PDF
HTML

References

Alfenas, A. C. (1998). Eletroforese de isoenzimas e proteínas afins: fundamentos e aplicações em plantas e microrganismos. Viçosa, MG: UFV.

Alscher, R. G, Erturk, N., & Health, L. S. (2002). Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany, 53, 1331-1341.

Amarante, C. V. T., Mota, C. S., Megguer, C. A., & Ide, G. M. (2007). Conservação pós-colheita de pinhões [sementes de Araucaria angustifolia (Bertoloni) Otto Kuntze] armazenados em diferentes temperaturas. Ciência Rural, 37, 346-351.

Auler, N. M. F., Reis, M. S., Guerra, M. P., & Nodari, R. O. (2002). The genetics and conservation of Araucaria angustifolia: I. Genetic structure and diversity of natural populations by means of non-adaptive variation in the state of Santa Catarina, Brazil. Genetics and Molecular Biology, 25, 329-338.

Balbuena, T. S., Silveira, V., Junqueira, M., Dias, L. L. C., Santa-Catarina, C., Shevchenko, A., & Floh, E. I. S. (2009). Changes in the 2-DE protein profile during zygotic embryogenesis in the Brazilian Pine (Araucaria angustifolia). Journal of Proteomics, 72, 337-352.

Balbuena, T. S., Jo, L., Pieruzzi, F. P., Dias, L. L. C., Silveira, V., Santa-Catarina, C., ..., & Floh, E. I. S. (2011). Differential proteome analysis of mature and germinated embryos of Araucaria angustifolia. Phytochemistry, 72, 302-311.

Barbedo, C. J., & Marcos Filho, J. (1998). Tolerância à dessecação de sementes. Acta Botânica Brasilica, 12, 145-164.

Barreiros, A. L. B. S., David, J. M., & David, J. P. (2006). Estresse oxidativo: relação entre geração de espécies reativas e defesa do organismo. Química Nova, 29, 113-123.

Bewley, J. D., & Black, M. (1994). Seeds: physiology of development and germination. New York, NY: Plenum Press.

Blokhina, O., Virolainen, E., & Fagerstedt, K. V. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany, 91, 179-194.

Boubriak, I., Kargiolaki, H., Lyne, L., & Osborne, D. J. (1997). The requirement for DNA repair in desiccation tolerance of germinating embryos. Seed Science Research, 7, 97-105.

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248-254.

Chiari, L., Valle, J. V. R., & Resende, R. M. S. (2009). Comparação de três métodos de extração de DNA genômico para análises moleculares em Stylosanthes guianensis. Campo Grande, MS: Embrapa-CNPGC.

Ching, T. M., & Schoolcraft, I. (1968). Physiological and chemical differences in aged seeds. Crop Science, 8, 407-409.

Coolbear, P. (1995). Mechanisms of seed deterioration. In A. S. Basra (Ed.), Seed quality: basic mechanisms and agricultural implications (pp. 223-277). New York, NY: Food Products Press.

Corbineau, F. (2012). Markers of seed quality: from present to future. Seed Science Research, 22, S61-S68.

Corte, V. B., Borges, E. E. L., Leite, H. G., Pereira, B. L. C., & Gonçalves, J. F. C. (2010). Estudo enzimático da deterioração de sementes de Melanoxylon brauna submetidas ao envelhecimento natural e acelerado. Revista Brasileira de Sementes, 32, 83-91.

Doyle, J. J., & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.

Doyle, J. J., & Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13-15.

Epagri/Ciram. (2011). Atlas climatológico do estado de Santa Catarina. Retrieved from http://www.ciram.epagri.rct-sc.br

Farias-Soares, F. L., Burrieza, H. P., Steiner, N., & Guerra, M. P. (2013). Immunoanalysis of dehydrins in Araucaria angustifolia embryos. Protoplasma, 250, 911-918.

Ferrandis, P., Bonilla, M., & Osorio, L. C. (2011). Germination and soil seed bank traits of Podocarpus angustifolius (Podocarpaceae): an endemic tree species from Cuban rain forests. Revista de Biología Tropical, 59(3), 1061-1069.

Fowler, J. A. P., Bianchetti, A., & Zanon, A. (1998). Conservação de sementes de pinheiro-do-paraná sob diferentes condições de ambientes e embalagens. Embrapa: Comunicado Técnico, 34, 1-4.

Garcia, C., Coelho, C. M. M., Maraschin, M., & Oliveira, L. M. (2014). Conservação da viabilidade e vigor de sementes de Araucaria angustifolia (Bert.) O. Kuntze durante o armazenamento. Ciência Florestal, 24(4), 857-866.

Gill, S. S., & Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48, 909-930.

Halliwell, B., & Gutteridge, J. M. C. (1999). Free Radicals in Biology and Medicine. New York, NY: Oxford University Press.

José, S. C. B. R, Salomão, A. N., Costa, T. S. A., Silva, J. T. T. T., & Curi, C. C. S. (2010). Armazenamento de sementes de girassol em temperaturas subzero: aspectos fisiológicos e bioquímicos. Revista Brasileira de Sementes, 32, 29-38.

Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.

Li, D. Z., & Pritchard, H. W. (2009). The science and economics of ex situ plant conservation. Trends in Plant Science, 14, 614-621.

Löwe, T. R., & Dillenburg, L. R. (2011). Changes in light and nutrient availabilities do not alter the duration of use of seed reserves in Araucaria angustifolia seedlings. Australian Journal of Botany, 59, 32-37.

McDonald, M. B. (1999). Seed deterioration: phisiology, repair and assessment. Seed Science and Technology, 27, 177-237.

Misra, H. P., & Fridovich, I. (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biology and Chemistry, 247, 188-192.

Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends Plant Science, 7, 405-410.

Murthy, U. M. N., Kumar, P. P., & Sun, W. Q. (2003). Mechanisms of seed ageing under different storage conditions for Vigna radiata (L.) Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition. Journal of Experimental Botany, 54, 1057-1067.

Nakada, P. G., Oliveira, J. A., Melo, L. C., Silva, A. A., Silva, P. A., & Perina, F. J. (2010). Desempenho durante o armazenamento de sementes de pepino submetidas a diferentes métodos de secagem. Revista Brasileira de Sementes, 32, 42-51.

Ohkawa, H., Ohishi, H., & Yagi, K. (1979). Assay for lipid peroxyde in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95, 351-358.

Pammenter, N. W., & Berjak, P. (2000). Aspects of recalcitrant seed physiology. Revista Brasileira de Fisiologia Vegetal, 12, 56-69.

Paula, N. F., Borges, E. E. L., Borges, R. C. G., & Paula, R. C. (1997). Alterações fisiológicas em sementes de seringueira (Hevea brasiliensis Muell. Arg.) durante o armazenamento. Revista Brasileira de Sementes, 19, 327-334.

Paula, N. F., Borges, E. E. L., Borges, R. C. G., & Paula, R. C. (1998). Avaliações bioquímicas e fisiológicas em sementes de seringueira (Hevea brasiliensis Muell. Arg.). Revista Brasileira de Sementes, 20, 1-10.

Rendón, M. Y., Gratão, P., Salva, T. J. G., Azevedo, R. A., & Bragagnolo, N. (2013). Antioxidant enzyme activity and hydrogen peroxide content during the drying of Arabica coffee beans. European Food Research and Technology, 236, 753-758.

Rosenfield, M. F., & Souza, A. F. (2014). Forest biomass variation in Southernmost Brazil: the impact of Araucaria trees. Revista de Biología Tropical, 62(1), 359-372.

Santos, C. M. R., Menezes, N. L., & Villela, F. A. (2004). Alterações fisiológicas e bioquímicas em sementes de feijão envelhecidas artificialmente. Revista Brasileira de Sementes, 26, 110-119.

SAS. (2009). SAS - Statistical Analysis System (Version 9.2). Cary, NC, Lic. UDESC: SAS Institute Inc.

Shibata, M., Coelho, C. M. M., Oliveira, L. M., & Garcia, C. (2012). Accelerated aging of ipê seeds under controlled conditiond of storage. Revista Brasileira de Sementes, 34, 247-254.

Smith, M. T., & Berjak, P. (1995). Deteriorative changes associated with loss of viability of stored dessication-tolerant and dessication-sensitive seeds. In J. Kiegel, & G. Galili (Eds.), Seed development and germination (pp. 701-746). New York, NY: Marcel Dekker Inc.

Song, S. Q., Berjak, P., Pammenter, N., Ntuli, T. M., & Fu, J. R. (2003). Seed recalcitrance: a current assessment. Acta Botania Sinica, 45, 638-643.

Steiner, N. (2005). Parâmetros fisiológicos e bioquímicos durante e embriogênese zigótica e somática de Araucaria angustifolia (Bert.) O. Kuntze (Master's degree), Federal University of Santa Catarina, Florianópolis.

Thomas, P. (2013). Araucaria angustifolia. In IUCN Red List of Threatened Species. Version 2013.2. Retrieved from http://www.iucnredlist.org

Veloso, H. P., Rangel Filho, A. L. R., & Lima, J. C. A. (1991). Classificação da vegetação brasileira, adaptada a um sistema universal. Rio de Janeiro, RJ: IBGE - Fundação Instituto Brasileiro de Geografia e Estatística.

Vibrans, A. C., Sevegnani, L., Uhlmann, A., Schorn, L. A., Sobral, M. G., Gasper, A. L., …, & Verdi, M. (2011). Structure of mixed ombrophyllous forests with Araucaria angustifolia (Araucariaceae) under external stress in Southern Brazil. Revista de Biologia Tropical, 59(3), 1371-1387.

Vidas, R. M. R., Moreira, M. A., Pinheiro, W. J., Rocha, V. S., Rezende, S. T., & Sediyama, C. S. (1992). Relação entre vigor e alterações bioquímicas na germinação de sementes de soja. Revista Brasileira de Fisiologia, 4, 49-53.

Villela, F. A., & Peres, W. B. (2004). Coleta, beneficiamento e armazenamento. In A. G. Ferreira & F. Borghetti (Eds.), Germinação: do básico ao aplicado (pp. 265-281). Porto Alegre, RS: Artmed.

Willan R. L. (1985). A guide to forest seed handling: with special reference to the tropics. Roma: Food and agriculture organization of the United Nations.

Wilson, D. O., & McDonald, M. B. (1986). The lipid peroxidation model of seed aging. Seed Science and Technology, 14, 296-300.

Comments

Downloads

Download data is not yet available.