Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://revistas.ucr.ac.cr/index.php/rbt/oai
Effect of storage temperatures on growth and survival of <i>Escherichia coli</i> O157: H7 inoculated in foods from a neotropical environment
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Keywords

escherichia coli O157
H7
meat
milk
cabbage
chicken giblets
survival

How to Cite

Arias, M. L., Monge-Rojas, R., Chaves, C., & Antillón, F. (2001). Effect of storage temperatures on growth and survival of <i>Escherichia coli</i> O157: H7 inoculated in foods from a neotropical environment. Revista De Biología Tropical, 49(2), 517–524. Retrieved from https://revistas.ucr.ac.cr/index.php/rbt/article/view/17450

Abstract

Escherichia coli O157: H7 has emerged as a new pathogen and is found worldwide. We studied the effect of several storage temperatures on the survival of this bacterium in common foods from a neotropical environment (Costa Rica) because at least seven clinical cases have been reported from the country, and no epidemiological link or probable food association has been described. High (106-108 CFU/ml) and low (104-106 CFU/ml) populations of E. coli were inoculated (three replications) in ground meat, chopped cabbage, chicken giblets and pasteurized milk and incubated at 0, 6 and 12ºC for 24, 48 and 72 h. Vegetables and milk were also stored at 22ºC for the same periods. The E. coli O157: H7 enumeration was done according to the methodology described in the Bacteriological Analytical Manual. Populations of E. coli O157: H7 showed either an increasing or decreasing trend, according to temperature, time or food base. Our data indicate that E. coli O157: H7 is capable of surviving and growing in meat, cabbage, milk and chicken giblets; food items commonly consumed by Costa Ricans.
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