Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

Histological analysis after arm tip amputation in the brittle star Ophioplocus januarii (Echinodermata: Ophiuroidea).


brittle star
histological analysis
estrella frágil
análisis histológico

How to Cite

Di Giorgio, G., Rubilar, T., & Brogger, M. I. (2016). Histological analysis after arm tip amputation in the brittle star Ophioplocus januarii (Echinodermata: Ophiuroidea). Revista De Biología Tropical, 63(S2), 297–308.


Wound healing in the arm tip after amputation in the brittle star Ophioplocus januarii was examined in San José gulf, Argentina, to observe histological and morphological changes. The process of wound healing is described and compared with other brittle stars species. This process in O. januarii involves the closure of the wound, the repair of the tissue damage and posterior formation of a presumptive stalked blastema by undifferentiated cells, sclerocytes and presumptive blastemic cells. The sclerocytes are the most abundant cell type due to this cells are crucial to the future skeleton regeneration. The results found in this study support the results found in others brittle stars.


Alves, S. L. S., Pereira, A. D., & Ventura, C. R. R. (2002). Sexual and asexual reproduction of Coscinasterias tenuispina (Echinodermata: Asteroidea) from Rio de Janeiro, Brazil. Marine Biology, 140, 95-101.

Aronson, R. B. (1987). Predation on fossil and recent ophiuroids. Paleobiology, 13, 187-192.

Aronson, R. B. (1991). Predation, physical disturbance, and sublethal arm damage in ophiuroids: a Jurassic-Recent comparison. Marine Ecology Progress Series, 74, 91-97.

Bartsch, I. (1982). Ophiuroidea (Echinodermata) from the Patagonian shelf. Mitteilungenausdem Hamburgischen Zoologischen Museum und Institut, 79, 211-250.

Biressi, A. C. M., Zou, T., Dupont, S., Dahlberg, C., Di Benedetto, C., Bonasoro, F., … Candia-Carnevali, M. D. (2010). Wound healing and arm regeneration in Ophioderma longicaudum and Amphiura filiformis (Ophiuroidea, Echinodermata): comparative morphogenesis and histogenesis. Zoomorphology, 129, 1-19.

Brogger, M. I. (2010). Crecimiento, reproducción y alimentación del ofiuroideo Ophioplocus januarii (Lütken, 1856) (Echinodermata: Ophiuroidea) en costas de Patagonia, Argentina (Tesis de doctorado). Universidad de Buenos Aires, Buenos Aires, Argentina.

Brogger, M. I., Gil, D. G., Rubilar, T., Martinez, M. I., Díaz de Vivar, M. E., Escolar, M., Epherra, L., Pérez, A. F., & Tablado, A. (2013). Echinoderms from Argentina: Biodiversity, distribution and current state of knowledge. In J. J. Alvarado & F. Solís-Marín (Eds.), Echinoderm Research and Diversity in Latin America (pp. 359-402). Berlin: Springer.

Byrne, M. (1994). Ophiuroidea. In F. W. Harrison & F. S. Chia (Eds.), Microscopic Anatomy of Invertebrates, Vol 14: Echinodermata (pp. 247-343). New York: Wiley-Liss, Inc.

Candelaria, A. G., Murray, G., File, S. K., & García Arrarás, J. (2006). Contribution of mesenterial muscle dedifferentiation to intestine regeneration in the sea cucumber Holothuria glaberrima. Cell and Tissue Research, 325, 55-65.

Candia-Carnevali, C. M. D. (2006). Regeneration in Echinoderms: repair, regrowth, cloning. Invertebrate Survival Journal, 3, 64-76.

Candia-Carnevali, C. M. D., & Bonasoro, F. (2001). Introduction to the biology of regeneration in echinoderms. Microscopy Research and Technique, 55, 365-368.

Candia-Carnevali, C. M. D., Bonasoro, F., Patruno, M., & Thorndyke, M. C. (1998). Cellular and molecular mechanisms of arm regeneration in crinoid echinoderms: the potential of arm explants. Development Genes and Evolution, 208, 421-430.

Clark, M. S., Dupont, S., Rossetti, H., Burn, G., Thorndyke, M. C., & Peck, L. S. (2007). Delayed arm regeneration in the Antarctic brittle star Ophionotus victoriae. Aquatic Biology, 1, 45-53.

Dupont, S., & Thorndyke, M. C. (2006). Growth or differentiation? Adaptive regeneration in the brittlestar Amphiura filiformis. Journal of Experimental Biology, 209, 3873-3881.

Emson, R. H., & Wilkie, I. C. (1980). Fission and autotomy in Echinoderms. Oceanography and Marine Biology, an Annual Review, 18, 155-250.

García Arrarás, J. E., Estrada Rodgers, L., Santiago, R., Torres, I., Díaz Miranda, L., & Torres Avillan, I. (1998). Cellular mechanisms of intestine regeneration in the sea cucumber, Holothuria glaberrina Selenka (Holothuroidea: Echinodermata). Journal of Experimental Biology, 281, 288-304.

Lawrence, J. M. (1987). A functional biology of echinoderms. London: Croom Helm Ltd.

Lawrence, J. M., Byrne, M., Harris, L., Keegan, B., Freeman, S., & Cowell, B. C. (1999). Sublethal arm loss in Asterias amurensis, A. rubens, A. vulgaris, and A. forbesi (Echinodermata: Asteroidea). Vie et Milieu, 49, 69-73.

Lawrence, J. M., & Vasquez, J. (1996). The effect of sublethal predation on the biology of echinoderms. Oceanologica Acta, 19, 431-440.

Märkel, K., & Röser, U. (1985). Comparative morphology of echinoderm calcified tissues: Histology and ultraestructure of ophiuroid scales (Echinodermata, Ophiuroidea) Zoomophology, 105, 197-207.

Mashanov, V. S., & Dolmatov, I. Y. (2000). Developmental morphology of a holothurian, Cucumaria japonica (Dendrochirota, Holothuroidea), a species with accelerated metamorphosis. Invertebrate Reproduction and Development, 37, 137-146.

Pomory, C. M., & Lawrence, J. M. (1999). Effect of arm regeneration on energy storage and gonad production in Ophiocoma echinata (Echinodermata: Ophiuroidea). Marine Biology, 135, 57-63.

Pomory, C. M., & Lawrence, J. M. (2001). Arm regeneration in the field in Ophiocoma echinata (Echinodermata: Ophiuroidea): effects on body composition and its potential role in a reef food web. Marine Biology, 139, 661-670.

Rodríguez, M. S. (2009). Estudios de crecimiento en el ofiuroideo Ophioplocus januarii (Echinodermata: Ophiuroidea) del Golfo San José, Patagonia. (Tesis de licenciatura). CAECE, Buenos Aires, Argentina.

Rubilar, T., Pastor de Ward, C. T., & Díaz de Vivar, M. E. (2005). Sexual and asexual reproduction of Allostichaster capensis (Echinodermata: Asteroidea) in Golfo Nuevo. Marine Biology, 146, 1083-1090.

Rubilar, T. (2010). Morfología del sistema nervioso y regeneración en el asteroideo fisíparo Allostichaster capensis. (Tesis de doctorado). Universidad Nacional del Comahue, Bariloche, Argentina.

Sánchez Alvarado, A., & Tsonis, P. A. 2006. Bridging the regeneration gap: genetic insights from diverse animal models. Nature Reviews Genetics, 7, 873-884.

Smith, V. J. (1981). The echinoderms. In N. A. Ratcliffe & A. F. Rowley (Eds.), Invertebrate Blood Cells (pp. 513-562). London: Academic Press.

Sokal, R. R., & Rohlf, F. J. (1979). Biometría: principios y métodos estadísticos en la investigación biológica. Madrid: H. Blume.

Thomas, L. P. (1975). The systematic relationships of Ophioplocus, Ophioceramis, and Ophioceres (Echinodermata: Ophiuroidea). Bulletin of Marine Science, 25, 232-247.

Tommasi, L. R. (1968). Os Ofiuróides existentes nas coleçoes do Museu de Buenos Aires coletados do La Plata até 42º S. Papéis avulsos de Zoologia, 21, 115-124.

Tommasi, L. R. (1970). Os Ofiuróides recentes do Brasil e de regioes vizinhas. Contribuições Avulsas do Instituto Oceanográfico da Universidade de São Paulo, 20, 1-146.

Yokoyama, H. (2008). Initiation of limb regeneration: The critical steps for regenerative capacity. Development Growth and Differentiation, 50, 13-22.



Download data is not yet available.