Keywords
air layers
cuttings
intermediate seeds
germination
Phyllonoma laticuspis
acodos
estacas
semillas intermedias
germinación.
How to Cite
Abstract
Phyllonoma laticuspis leaves are used in Carrizal de Bravo, Guerrero, Mexico, to heal skin lesions such as injuries and smallpox sequelae and to treat diabetes mellitus type 2, and organic extracts of these leaves have been reported to exert antibacterial effects. High demand of P. laticuspis as a medicinal plant has decreased its natural populations, and propagation of the species has not yet been reported. Therefore, the purpose of this study was to assess the vegetative propagation of the species through cutting and air layering, as well as its sexual propagation in a preserved population. For this, concentrations of 1 000, 4 000 and 6 000 ppm of a commercial root enhancer, with indole butyric acid (IBA), and a control treatment without IBA, were applied to the cuttings and air layers. Germination was evaluated under light and dark conditions using lots of freshly collected seeds and lots of seeds that had been stored for three months at 4 °C or 24 ± 2 °C. All experiments were performed in a completely randomized design. The cuttings did not develop roots in any concentration, whereas 100 % of the air layers rooted, displaying vigorous roots in the presence of 4 000 ppm IBA, after four month of treatment application. Regarding germination, more than 60 % of the freshly collected seeds germinated, whereas less than 20 % of the seeds stored at 4 °C, and close to 50 % of the seeds stored at 24 ± 2 °C germinated under light and dark conditions. No significant differences were observed between light and dark conditions, so they were categorized as indifferent photoblastic seeds. The observed moisture content of 13.5 % and germination behaviour as the response to cold storage, suggest that the resultant seed quality was intermediate. P. laticuspis propagation for short-term foliage production can be carried out in air layers, in populations with a high density of adult plants as a source of plant material and for the restoration of disturbed areas, in the same locality. On the other hand, large-scale seedling production, medium-term foliage production and preservation of species variability can be achieved using seeds.
References
Alves-de Oliveira, A., Carlos-Koller, O., & Villegas-Monter, A. (1999).Propagación vegetativa de aguacate selección 153 (Persea sp.) por acodo en contenedor. Revista Chapingo Serie Horticultura, 5, 221-225.
Bell, K. L., & Bliss, L. C. (1980). Plant reproduction in a high artic environment. Artic and Alpine Research, 12, 1-10.
Blazich, F. A. (1988). Chemicals and formulations used to promote adventitious rooting. In T. D. Davis, B. E. Haissig, & N. Sankhla (Eds.), Adventitious root formation in cuttings (pp. 132-149). Portland, Oregon: Dioscorides.
Cordero, R. A. (2000). Effect of two naturallight regimes and nutrient addition on the forest herb Begonia decandra (Begoniaceae). Revista de Biología Tropical, 48, 579-586.
Corner, E. J. H. (1976). The Seeds of Dicotyledons. London, Great Britain: Cambridge University Press.
Chong, C., Allen, O. B., & Barnes, H. W. (1992). Comparative rooting of stem cuttings of selected woody landscape shrub and tree taxa to varying concentrations of IBA in talc, ethanol and glycol carriers. Journal of Environmental Horticulture, 10, 245-250.
De Klerk, G. J., Van der Krieken, W., & De Jong, J. C. (1999). The formation of adventitious roots: new concepts, new possibilities. In Vitro Cellular & Developmental Biology – Plant, 35, 189-199.
Ellis, R. H., Hong, T. D., & Roberts, E. H. (1990). An intermediate category of seed storage behaviour? I. Coffee. Journal of Experimental Botany, 41, 1167-1174.
Escobar, D. F., & Torres, A. M. (2013). Morphology, ecophysiology and germination of seeds of the Neotropical tree Alibertia patinoi (Rubiaceae). Revista de Biología Tropical, 61, 547-556.
Eurola, S. (1972). Germination of seeds collected in Spitsbergen. Annales Botanici Fennici, 9, 149-159.
Ferrandis, P., Bonilla, M., & Osorio, L. C. (2011). Germination and soil seed bank traits of Podocarpus angustifolius (Podocarpaceae): an endemic tree species from Cuban rain forests. Revista de Biología Tropical, 59, 1061-1069.
Figueroa, J. A., & Vázquez-Yanes, C. (2002). Efecto de la calidad de la luz sobre la germinación de semillas en el árbol pionero tropical Heliocarpus appendiculatus (Tiliaceae). Revista de Biología Tropical, 50, 31-36.
Haisig, B. E. (1974). Origins of adventitious roots. Journal of Forestal Science, 4, 299-310.
Hartmann, H. T., & Kester, D. E. (1989). Propagación de Plantas. Principios y prácticas. México, Calzada de Tlalpan 5022, México 22, D. F.: Compañía Editorial Continental, S. A. de C. V.
Hossain, M. A., Islam, M. A., & Hossain, M. M. (2004). Rooting ability of cuttings of Swietenia macrophylla King and Chukrasia velutina Wight et Arn as influenced by exogenous hormone. International Journal of Agriculture & Biology, 6, 560-564.
Howard, B. H., & Nahlawi, N. (1969). Factors affecting the rooting of plum Harwood cuttings. Journal of Horticultural Science, 44, 303-310.
INEGI (Instituto Nacional de Estadística y Geografía) (2000). www.inegi.gob.mx.
LaPierre, L. M. (2001). Vegetative propagation of Cecropia obtusifolia (Cecropiaceae). Revista de Biología Tropical, 49, 973-976.
Leakey, R. R. B., Chapman, V. R., & Longman, K. A. (1982). Physiological studies for tropical tree improvement and conservation. Factors affecting root initiation in cuttings of Triplochiton scleroxylon K. Schum. Forest Ecology and Management, 4, 53-66.
León-Lobos, P., & Ellis, R. H. (2002). Seed storage behavior of Fagus sylvatica and Fagus crenata. Seed Science Research, 12, 31-37.
Martínez, M. (1969). Las Plantas Medicinales de México. México D.F.: Ediciones Botas.
Martínez-Camilo, R., Pérez-Farrera, M. A., & Martínez-Meléndez, N. (2012). Listado de plantas endémicas y en riesgo de la Reserva de la Biosfera El Triunfo, Chiapas, México. Botanical Sciences, 90, 263-285.
Martínez-Gordillo, M., Cruz-Durán, R., Castrejón-Reyna, J. P., Jiménez-Ramírez, J., & Ruíz-Jiménez, C. A. (2004). Flora Vascular de la porción Guerrerense de la Sierra de Taxco, Guerrero, México. Anales del Instituto de Biología. Serie Botánica, 75, 105-189.
Mesén, F., Newton, A. C., & Leakey, R. R. B. (1997). Vegetative propagation of Cordia alliodora (Ruiz & Pavon) Oken: the effects of IBA concentration, propagation medium and cutting origin. Forest Ecology and Management, 92, 45-54.
Mesén, F. (1998). Enraizamiento de estacas juveniles de especies forestales: uso de propagadores de sub-irrigación (Serie técnica, Manual Técnico No. 30). Turrialba, Costa Rica: CATIE.
Paton, D. M., Willing, R. R., Nicholls, W., & Pryor, L. D. (1970). Rooting of stem cuttings of Eucalyptus: A rooting inhibitor in adult tissue. Australian Journal of Botany, 18, 175-183.
Pérez-Calix, E. (1999). Phyllonomaceae. Flora del Bajío y de Regiones Adyacentes. Pátzcuaro, Michoacán, México: Instituto de Ecología, A.C. Centro Regional del Bajío.
Rochefort, L., & Vitt, D. H. (1990). Growth, production, and decomposition dynamics of Sphagnum under natural and experimentally acidified conditions. Ecology, 71, 1986-2000.
Ruiz-García, R., Vargas-Hernández, J. J., Cetina-Alcalá, V. M., & Villegas-Monter, A. (2005) Efecto del Ácido Indolbutírico (AIB) y tipo de estaca en el enraizado de Gmelina arborea Roxb. Revista Fitotecnia Mexicana, 28, 319-326.
Sahoo, N., Manchikanti, P., & Dey, S. (2010). Herbal drugs: Standards and regulation. Fitoterapia, 81, 462-71.
Sánchez, J. A., Muñoz, B., & Montejo, L. (2003). Efectos de tratamientos robustecedores de semillas sobre la germinación y establecimiento de árboles pioneros bajo condiciones de estrés. Ecotrópicos, 16, 91-112.
Stefancic, M., Stampar, F., & Osterc, G. (2005). Influence of AIA on root development and quality of Prunus ‘GiSe1A5’ leafy cuttings. HortScience, 40, 2052-2055.
Tchoundjeu, Z., Avana, M. L., Leakey, R. R. B., Simons, A. J., Assah, E., Duguma, B., & Bell, J. M. (2002). Vegetative propagation of Prunus africana: effects of rooting medium, auxin concentrations and leaf area. Agroforestry Systems, 54, 183-192.
Vázquez-Yanes, C., & Orozco-Segovia, A. (1992). Effects of litter from a tropical rainforest on tree seed germination and establishment under controlled conditions. Tree Physiology, 11, 391-400.
Vega-Castro, C. (2002). Análisis de los extractos orgánicos de la lámina foliar de Phyllonoma laticuspis (Phyllonomaceae) una especie empleada en la medicina tradicional (Tesis de Licenciatura). Universidad Nacional Autónoma de México, México, D. F.
Comments
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2017 Revista de Biología Tropical