https://revistas.ucr.ac.cr/index.php/rbtRevista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

Aeromonas spp. y Plesiomonas shigelloides en bivalvos, cieno y aguas del Golfo de Nicoya, Costa Rica

Evelyn Rodríguez, Florencia Antillón G.

Abstract


Bivalves, mud, and surface water were collected at three different sites of the Gulf of Nicoya, Costa Rica, in search of Aeromonas spp. and Plesiomonas shigelloides. For their isolation, these bacteria were enriched in alkaline peptone water and streaked on MacConkey agar and on brilliant green bile inositol agar. This was followed by the biochemical tests necessary for their identification. Thirty-five strains of A. hydrophila, 58 of A. caviae, 43 of A. sobria, and 7 of P. shigelloides were isolated. None of these predominated nor was there any indication of a seasonal distribution along the 15 month's duration of the study. Seven strains of A. hydrophila and two of A. sobria showed the biochemical characteristics associated with toxin production (positive Voges-Proskauer and lysine decarboxylase tests). These species are widely distributed in the gulf and there is risk of contracting an infection while bathing or when eating raw bivalves from this area

References


Altwegg, M. & M. Johl. 1987. Isolation frequency of Aeromonas spp. in relation to patient's age. Eur. J. Clin. Microbiol. 6: 55-56.

Arai, T., N. Ikejima, T. Itoh, S. Sakai, T. Shimada & R. Sakazaki. 1980. A survey of Plesiomonas shigelloides from aquatic enviroments, domestic animals, pets, and humans. J. Hyg. 84: 203-212.

Buchanan, R.L. & S.A. Palumbo. 1985. Aeromonas Ilydrophila and Aeromonas sobria as potential food poisoning species: a review. J. Food Safety 7: 15-29.

Burke V., M. Gracey. J. Robinson, O. Peck, J. Beaman & C. Bundell. 1983. The microbiology of childhood gastroenteritis: Aeromonas species and other infective agents. J. Infect. Dis. 148: 68-74.

Burke. V., J. Rohinson, M. Gracey. O. Peterson & K. Partridge. 1984. Isolation of Aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates. Appl. Enviran. Microbiol. 48: 361-366.

Caltister, S. & W. Agger. 1987. Enumeration and characterization of Aeromonas hydrophila and Aeromonas caviae isolated from grocery store products. Appl. Environ. Microbiol. 53: 249-253.

Daily, O.P., S.W. Joseph, J.C. Coolbaugh, R.I. Walter, B.R. Merrell, D.M. Rollins, R.J. Seidler, R.R. Colwell & C.R. Lissner. 1981. Association of Aeromonas sobria with human infeetion. J. Clin. Microbiol. 13: 769-777.

Fernández, B. & T. Brunker. 1977. Estudio bacteriológico de bivalvos del Golfo de Nicoya, Costa Rica. I. Condición del molusco recién recolectado. Rev. Biol. Trop. 25: 101-107.

Fernández, B., T. Brunker & C. González. 1971. Calidad sanitaria de las aguas de la playa de Puntarenas. II. Primera recalificación. Acta Médica. Cost. 14: 91-100.

Fernández, B. & K. Ryan. 1983. Estudio bacteriológico de bivalvos del Golfo de Nicoya, Costa Rica. II. Condición del molusco al momento de comercio. Rev. Biol. Trop. 31: 311-316.

Hazen, T.C., C.B. Fliermans, R.P. Hirsch & G.H. Esch. 1978. Prevalence and distribution of Aeromonas hydrophila in the United States. Appl. Environ. Microbiol. 36: 731-738.

Herrera. M.L., J. Guevara, J.L. Salas y C. Lizano. 1987. Aislamiento extraintestinal de Aeromonas hydrophilo en Costa Rica. Rev. Med. Hosp. Nal. de Niños Costa Rica. 1: 29-34.

Hunt D., J. Miescier. J. Redman. A. Salinger & J. Lucaso 1984. Molluscan shellfish, fresh or freshfrozen oyslers, mussels, or clams. p. 590-607. In M. Speck (ed.) Compendium of methods for the microbiological examination of foods. Second ed. American Public Health Association. Washington. D.C.

Kirov S.M., E. Rees. R.C. Wellock. J.M. Goldsmid & A.D. Galen. 1986. Virulence characteristics of Aeromonas spp. in relation to source and biotype. J. Clin. Microbiol. 24: 827-834.

Moyer, N.P. 1987. C1inical significance of Aeromonas species isolated form patients with diarrhea. J. Clin. Microbiol. 25: 2044-2048.

Pathak, A., J.R, Custer & J. Leuy. 1983, Neonatal septicemia and meningitis due to Plesiomonas shigelloides, Pediatrics 71: 389-391.

Popoff, M. 1984. Aeromonas, p. 545-548. In N.R. Krieg & J.C. Holt (ed.) Sergey's Manual of Systematic Bacteriology, vol. 1. The Williams Wilkins Co. Baltimore.

San Joaquín, V.H. & D.A. Pickett. 1988. Aeromonas associated gastroenteritis in children. Pediatr. Infect. Dis. 1. 7: 53-57.

Schubert. R. 1984, Plesiomonas, p. 548-550. In N.R. Krieg & J.G. Hoh (ed.), Bergey's Manual of Systematic Bacteriology, vol. 1. The Williams Wilkins Co., Baltimore.

Seidler, R.J. D.A. Allen, H. Lockman, R.R. Colwell, S.W. Joseph & O.P. Oaily. 1980. Isolalion, enumeration, and characterization of Aeromonas from polluted waters encountered in diving operations. Appl. Environ. Microbiol. 39: 1010-1018.

Settergren, B. K.A. Broholm & S. Norrby. 1986. Fatal infection with Aeromonas sobrio and Plesiomonas shigelloides. Br. Med. J. 292: 525-526.

Tsukamoto, T., Y. Kinoshita, T. Shimada & R. Sakazaki. 1978. Two epidemics of diarrhoeal disease possibly caused by Plesiomonas shigelloides. J. Hyg. 80: 275-280.

Turnbull. P., J. Lee, M.D. Miliotis, S. Van De Walle, H.J. Koornhof. L. Jeffery & T. Sryant. 1984. Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species. J. Clin. Microbiol. 19: 175-180.

Von Graevenitz. A. 1985. Aeromonas and Plesiomonas, p. 278-281. In E.H. Lennette, A. Balows. W.L Hausler, and H J. Shadomy (ed.). Manual of Clinical Microbiology. Fourth ed. American Society for Microbiology, Washington, D.C.

Van Graevenitz, A. & C. Bucher. 1983. Evaluation of differential and selective media for isolation of Aeromonas and Plesiomonas spp. from human feces. J. Clin. Microbiol. 17: 16-21.


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