Abstract
Lacteous fat globules with their membranes from human milk and calostrum were studied by scanning and transmission electron microscopy. The first appear as spheroidal structures with some irregularities on the surface. Under the transmission electron microscope these irregularities are composed of islets from a material morphologically similar to cytoplasm, and with structures that resemble a fragment of rugose endoplasmic reticulum. The membranes in specimens fixed immediately after secretion are tri-layered, similar in appearance to those of a single membrane, but in samples fixed between two and four hours after secretion the details are unclear. Through freeze-etching, the laminar aspect of the fat globules is observed.References
Baumiucker, D. R., & T.W. Keenan. 1973. Membranes of mammary gland. VII. Stability of milk fat globule membrane. J. Dairy Sci., 56: 1092-1094.
Baver, H. 1 972. Ultrastructure observations on the milk fat globule envelope of cow's milk. J. Dairy Sci., 55: 1375-1387.
Braceo, V., J. Hidalgo, & H. Bohren. 1 972. Lipid composition of the fat globule membrane of human and bovine milk. J. Dairy Sci., 55: 165-172.
Eigel, W.N. 1980. Characterization of bovine milk fat globule membrane prepared in the presence of chloroquine and kallikrein inhibition. J. Dairy Sci. 63: 158.
Horisberger, M., J. Rosset, & M. Vonlanthen. 1977. Location of glycoproteins on milk fat globule membrane by scanning and transmission electron microscopy, using lectinlabelled gold granules.
Exp. Cell. Res., 109: 361-369.
Keenan, T. W., D.J. Morré, D. E. Olson, W. N. Yunghans, & S. Patton. 1970. Biochemical and morphological comparison of plasma membrane and milk fat globule membrane from bovine mammary gland. J. Cell. Biol., 44: 80-93.
Keenan, T.W., D.E. Olson, & H. H. Mollenhaver. 1971. Origin of the milk fat globule membrane. J. Dairy Sci., 54: 295-299.
Long, C. A., & S. Patton. 1978. Formation of intracellular fat droplets: interrelation of newly synthesized phosphatidylcholine and triglyceride in milk. J. Dairy Sci., 61: 1392-1399.
Mather, L. H., C. B. Tamplin, C.S. Nace, V.G. Johnson, P.J. Madara, & R. A. Goldsby. 1980. The preparation of polyclonal and monoclonal antibodies to the proteins of bovine milk-fat globule membrane. J. Dairy Sci., 63 (Suppl.): 159-160.
Patton, S., C. Long, & T. Sokka, 1980. Effect of storing milk on cholesterol and phospholipid of skim milk. J. Dairy Sci., 63: 697-700.
Pillay, V.T., A. N. Myhr, & J.I. Gray. 1980a. Lipolysis in milk. I. Determination of free fatty acid and threshold value for lipolized flavor detection. J. Dairy Sci., 63: 1213-1218.
Pillay, V.T., A. N. Myhr, J. I. Gray, & D. A. Biggs. 1980b. Lipolysis in milk. II. Effect to milking systems. J. Dairy Sci., 63: 1219-1223.
Thompson, M. P., J. R. Brunner, C.M. Stine, & K. Lindquist. 1961. Lipid components of the fat globule membrane. J. Dairy Sci., 44: 1589-1596.
Wooding, F. B. P. 1971a. The structure of the milk fat globule membrane. J. Ultrastruct. Res., 37: 388-400.
Wooding, F. B. P. 1971b. The mechanism of secretion of milk fat globules. J. Dairy Sci., 9: 805-821.
Wooding, F. B. P. 1972. Milk microsomes, viruses, and the milk fat globule membrane. Experientia, 28: 1077-1079.
Zittle, C.A., E. S. Dellamonica, J. H. Custer, & R. K. Rudd. 1956. The fat-globule membrane of milk: alkaline phosphatase and xanthine oxidase in skim milk and cream. J. Dairy Sci., 39: 528-535.
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Copyright (c) 1983 Revista de Biología Tropical