Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

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Paralytic Shellfish Poisoning (PSP) in Margarita Island, Venezuela
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Keywords

Dinoflagellate
Paralytic Shellfish Poisoning
saxitoxin
Prorocentrum
Gymnodinium catenatum
Alexandrium tamarense
Dinoflagelado
intoxicación paralizante por moluscos
saxitoxina
Prorocentrum
Gymnodinium catenatum
Alexandrium tamarense

How to Cite

La Barbera-Sánchez, A., Franco Soler, J., Rojas de Astudillo, L., & Chang-Yen, I. (2004). Paralytic Shellfish Poisoning (PSP) in Margarita Island, Venezuela. Revista De Biología Tropical, 52(S1), 89–98. Retrieved from https://revistas.ucr.ac.cr/index.php/rbt/article/view/26522

Abstract

A severe outbreak of Paralytic Shellfish Poisoning (PSP) occurred in Manzanillo and Guayacán, northwestern coast of Margarita Island, Venezuela, between August and October 1991. A bloom of dinoflagellates including Prorocentrum gracile, Gymnodinium catenatum and Alexandrium tamarense seemed to be responsible for this outbreak. Levels of PSP toxins in mussels (Perna perna) exceeded the international safety limit of saxitoxin, 80 µg STX/100 g meat. PSP toxin values varied between 2 548 and 115 µg STX/100 g meat in Manzanillo, and between 1 422 and 86 µg STX/100 g meat in Guayacán. At both locations, the highest levels were detected in August, when 24 patients exhibited typical symptoms of PSP toxicity after consuming cooked mussels (16 required hospitalization). A high pressure liquid chromatographic (HPLC) procedure was recently used on the 1991 samples. The major toxin detected in samples of both locations was decarbamoyl saxitoxin (dcSTX), but low concentrations of saxitoxin were also found in Manzanillo samples. Gonyautoxins GTX1, GTX2 and GTX3 were detected only at Guayacán, while in both locations, decarbamoylgonyatouxin (dcGTX2,3) toxins were detected. These findings represent the first time that causative toxins of PSP in Venezuela have been chemically identified, and confirm the presence of dcSTX and dcGTX in mussels from the Caribbean Sea. The presence of dcSTX and dcGTX in shellfish is indicative that Gymnodinium catenatum was a causative organism for outbreak of PSP.
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References

Amzil, Z., M. Quilliam, T. Hu & J.L.C. Wright. 1999. Winter accumulation of paralytic shellfish toxins in digestive glands of mussels from Arcachon and Toulon (France) without detectable toxic plankton species revealed by interference in the mouse bioassay for lipophilic toxins. Nat. Toxins 7: 271-277.

Bates, H.A. & H. Rapoport. 1975. A chemical assay for saxitoxin, the paralytic shellfish poison. J. Agric. Food Chem. 23: 237-239.

Carreto, J., C. Elbusto, H. Sancho, M. Carignan, T. Yasumoto & Y. Oshima. 1996. Comparative studies on paralytic shellfish toxin profiles of marine snails, mussels and an Alexandrium tamarense isolate from the Mar del Plata coast (Argentina). Rev. Invest. Des. Pesq. 10: 101-107.

Casais, C. 1991. Gymnodinium catenatum toxins from mussels (Mytilus galloprovincialis). Environ. Technol. 12: 33-40.

Donker, S., M.I. Reyero, B. Reguera & J.M. Franco. 1997. Perfil de toxinas PSP de seis cepas de Gymnodinium catenatum de Galicia, pp. 69-76. In J.M Wyeites & F. Leira (eds.). V Reunión Ibérica sobre Fitoplancton Tóxico y Biotoxinas, Vigo, España.

Franco, J.M. & P. Fernández-Villa. 1993. Separation of paralytic shellfish toxins by reversed phase high performance liquid chromatography with postcolumn reaction and fluorimetric detection. Chromatographia 35: 613-620.

Genenah, A.A. & Y. Shimizu. 1981. Specific toxicity of paralytic shellfish poisons. J. Agric. Food Chem. 29: 1281-1291.

Hallegraeff, G.M. & S. Fraga. 1998. Bloom dynamics of the toxic dinoflagellate Gymnodinium catenatum, with emphasis on Tasmanian and Spanish coastal waters, pp. 125-152. In D.M. Anderson, A.D. Cembella & G.M. Hallegraeff (eds.). Physiological ecology of harmful algal blooms, Berlin.

Harada, T., Y. Oshima & T. Yasumoto. 1983. Natural occurence of decarbamoylsaxitoxin in Tropical dinoflagellate and bivalves. Agric. Biol. Chem. 47: 191-193.

Horwitz, W. 1980. Official methods of Analysis of the Association of Official Analytical Chemists. Washington D.C. pp. 298-299.

Hummert, C., M. Ritscher, K. Reinhardt & B. Luckas. 1997. Analysis of the characteristic PSP profiles of Pyrodinium bahamense and several strain of Alexandrium sp. by HPLC based on ion-pair chromatographic separation, post-column oxidation and fluorescence detection. Chromatographia 45: 312-316.

Jiang, T., Y. Yin, Z. Chen, Y. Luo, W. Huang & Y. Qi. 2000. HPLC analysis of paralytic shellfish toxins in shellfish from Daya Bay. Mar. Environ. Sci. 19: 16-19.

Kao, D.Y. 1993. Paralytic shellfish poisoning, pp. 75-86. In I.R. Falconer (ed.). Algal toxins in seafood and drinking water. San Diego, CA. Koehn, F.E., V.E Ghazarossian, E.J. Schantz, H.K Schnoes & F.M. Strong. 1981. Derivatives of saxitoxin. Bioorg. Chem. 10: 412-428.

La Barbera-Sánchez, A., S. Hall & E. Ferraz-Reyes. 1993. Alexandrium sp., Gymnodinium catenatum and PSP in Venezuela, pp. 281-285. In T.J. Smayda & Y. Shimizu (eds.). Toxic Phytoplankton Blooms in the Sea, Amsterdam.

Lassus, P., J.M. Fremy, M. Ledoux, M. Bardouil & M. Bohec. 1989. Patterns of experimental contamination by Protogonyaulax tamarensis in some french commercial shellfish. Toxicon 27: 1313-1321.

Lawrence, J.F. & B. Niedzwiadek. 2001. Quantitative determination of paralytic shellfish poisoning toxins in shellfish by using prechromatographic oxidation and liquid chromatography with fluorescence detection. J. AOAC Int. 84: 1099-1108.

Lawrence, J.F., C. Ménard & Ch. Cleroux. 1995. Evaluation of prechromatographic oxidation for liquid chromatography determination of Paralytic Shellfish Poisons in shellfish. J. AOAC Int. 78: 514-520.

LeDoux, M. & S. Hall. 2000. Proficiency testing of eight french laboratories in using the AOAC mouse bioassay for paralytic shellfish poisoning: Interlaboratory collaborative study. J. AOAC Int. 83: 305-310.

Mosher, H.S., E.A. Fuhrman, H.S. Buchwald & H.G. Fischer. 1964. Tarichatoxin-Tetrodoxin: A potent neurotoxin. Science 144: 1100.

Noguchi, T., J. Maruyama, Y. Onoue, K. Hashimoto & T. Ikeda. 1983. Toxins of mussels infested with Protogonyaulax catenella isolated from Senzaki bay. Yamaguchi Prefecture. Bull. Japan. Soc. Sci. Fish. 49: 499.

Oshima, Y. 1995. Post column derivatization liquid chromatographic method for paralytic shellfish toxins. J. AOAC Int. 78: 528-532.

Oshima, Y., K. Sugino, H. Itakura, M. Hirota & T. Yasumoto. 1990. Comparative studies on paralytic shellfish toxin profile of dinoflagellates and bivalves, pp. 391-396. In E. Graneli, B. Sundstrom, L. Edler & D.M. Anderson (eds.). Toxic marine Phytoplankton, New York.

Oshima, Y., M. Hasegawa, T. Yasumoto, G. Hallegraeff & S. Blackburn. 1987. Dinoflagellate Gymnodinium catenatum as the source of paralytic shellfish toxins in Tasmanian shellfish. Toxicon 25: 1105-1111.

Park, D., W.N. Adams, S. Graham & R. Jackson. 1986. Variability of mouse bioassay for determination of paralytic shellfish poisoning toxins. J. AOAC Int. 69: 547-549.

Quilliam, M.A., C. Dell'Aversaro & P. Hess. 2001. Analysis of PSP toxins by liquid chromatographymass spectrometry. 15th AOAC International Annual Meeting. September 9th-13th, Kansas City, Missouri.

Reyes-Vásquez, E., E. Ferraz-Reyes & E. Vásquez. 1979. Toxic dinoflagellate blooms in northeastern Venezuela during 1977, pp. 191. In D.L. Taylor, H.H. Seliger (eds.). Toxic Dinoflagellate Blooms, New York.

Sullivan, J., & M. Wekell. 1988. Detection of paralytic shellfish toxins, pp. 87-106. In T. Anthony (ed.). Handbook of Natural Toxins, New York.

Sullivan, J., M.M. Wekell & L. Kentala. 1985. Application of HPLC for the Determination of PSP toxins in shellfish. J. Food. Sci. 50: 26-29. Thibault, P., S.

Pleasance & M.V. Laycock. 1991. Analysis of paralytic shellfish poisons by capillary electrophoresis. J. Chromatogr. 542: 483-501.

Utermöhl, H. 1958. Zur vervollkommung der quantitativen phytoplankton-methodic. Mitt. Int. Verein. Theor. Angrew. Limnol. 9: 1-38.

Vale, P. & M.A.M. Sampayo. 2001. Determination of paralytic shellfish toxins in Portuguese shellfish by automated pre-column oxidation. Toxicon 39: 561-571.

Van den Top, H.J., A. Boenke, P.A. Burdaspal, J. Bustos, H.P. Van Egmond, T. Legards, A. Mesego, A. Mouriño, W.E. Paulsch & C. Salgado. 2000. The development of reference materials for paralytic shellfish poisoning toxins in lyophilized mussel. I: Interlaboratory studies of methods of analysis. Food Add. Contam. 17: 419-433.

Negri A.P., C.J.S. Bolch, S.I. Blackburn, M. Dickman, L.E. Lewellyn & S. Mendez. 2000. Paralityc shellfish toxins in Gymnodinium catenatum strains from six countries. Procceedings of the 9th International Conference on Harmful Algal Blooms (Tasmania). (Downloaded: September 8th, 2003, http://www.utas.edu.au/docs/plant_science/HAB200 0/poster_abstracts/docs/Negri_Andrew_P.html)

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