Keywords
Gymnodinium catenatum
PST
La Paz Bay
Gulf of California
Megapitaria squalida
Gymnodinium catenatum
PST
Bahía de La Paz
Golfo de California
How to Cite
Abstract
Occurrence and toxic profiles of paralytic shellfish toxins (PST) in the chocolata clam Megapitaria squalida were investigated. From December 2001 to December 2002, 25 clams were obtained monthly from Bahía de La Paz, Gulf of California. Additionally, net (20 μm) and bottle phytoplankton samples were also collected to identify toxic species. Toxins were analyzed by HPLC with post-column oxidation and fluorescence detection. Toxicity in the clam was low and varied from 0.14 to 5.46 μg/STXeq/100 g. Toxicity was detected in December, March, April, June, and August. Toxin profile was composed mainly by STX, GTX2, GTX3, dcGTX2, dcGTX3, C2, dcSTX and B1. Gymnodinium catenatum was the only PST-producing dinoflagellate identified in the phytoplankton samples throughout the study period. G. catenatum was observed mainly in net samples from December 2001 to December 2002; however, in bottle samples, G. catenatum was only observed in five months. Highest abundance (2 600 cells l-1) was observed in March and the lowest (160 cells l-1) in June. G. catenatum mainly formed two-cell chains and rarely four or eight. The presence of PST in net phytoplankton samples support the fact that G. catenatum is the main source of PST in the clams. This study represents the first report of PST toxins in the chocolata clam from Bahía de La Paz.References
Alonso-Rodríguez, R. & F. Paéz-Osuna. 2003. Nutrients, phytoplankton and harmful algal blooms in shrimp ponds: a review with special reference to the situation in the Gulf of California. Aquaculture 219: 317-336.
Band-Schmidt, C.J., J.J. Bustillos-Guzmán, I. Gárate- Lizárraga, C. Lechuga-Deveze, K. Reinhardt & B. Luckas. 2004. Profiles of paralytic shellfish toxin in strains of the dinoflagellate Gymnodinium catenatum and the scallop Argopecten ventricosus from Bahía Concepción, Gulf of California, Mexico. Harmful Algae (In press).
Balech, E. 1988. Los Dinoflagelados del Atlántico Sudoccidental. Publ. Espec. Inst. Esp. Oceanogr. Nº 1. Madrid, pp. 310.
Bricelj, V.M. & S.E. Shumway. 1998. Paralytic shellfish toxins in bivalve molluscs: occurrence, transfer kinetics, and biotransformations. Rev. Fish. Sci. 6: 315-383.
Cortés-Altamirano, R. & R. Alonso-Rodríguez. 1997. Mareas rojas durante 1997 en la Bahía de Mazatlán, Sinaloa, México. Cien. Mar. 15: 31-37.
Cortés-Altamirano, R., D.U. Hernández-Becerril & R. Luna-Soria. 1996. Red tides in México: Areview, pp. 101-105. In T. Yasumoto, Y. Oshima & Y. Fukuyo (eds.). Harmful and Toxic Algal Blooms. Intergovernmental Oceanographic Commission, UNESCO, Sendai, Japan.
Cortés-Altamirano, R., A. Núñez-Pastén & N. Pastén- Miranda. 1999. Abundancia anual de Gymnodinium catenatum Graham dinoflagelado tóxico de la costa este del Golfo de California. Cien. Mar 35: 51-56.
Dodge, J.D. 1982. Marine Dinoflagellates of the British Isles. Her Majesty’s Stationery Office, London. pp. 303.
Figueroa-Torres, M.G. & M.A. Zepeda-Esquivel. 2001. Mareas rojas del Puerto Interior, Colima, México. Sci. Nat. 3: 39-52.
Fraga, S., S.M. Gallagher & D.M. Anderson.1998. Chainforming dinoflagellates: an adaptation to red tides, pp. 281-284. In T. Okaichi, D.M. Anderson & T. Nemoto (eds.). Red Tides: Biology, Environmental Science and Toxicology. Elsevier, New York.
Fukuyo, Y., H. Takano, M. Chihara & K. Matsuoka. 1990. Red Tide Organisms in Japan. An Illustrated Taxonomic Guide. Uchida Rokakuho, Tokyo. pp. 407.
Gárate-Lizárraga, I. & A. Martínez-López. 1997. Primer registro de una marea roja de Prorocentrum mexicanum (Prorocentaceae) en aguas del Golfo de California. Rev. Biol. Trop. 45: 1263.
Gárate-Lizárraga, I., M.L. Hernández-Orozco, C. Band- Schmidt & G. Serrano-Casillas. 2001. Red tides along the coasts of Baja California Sur, Mexico (1984 to 2001). Oceánides 16: 127-134.
Gárate-Lizárraga, I., D.A. Siqueiros-Beltrones & V. Maldonado- López. 2003. First record of a Rhizosolenia debyana bloom in the Gulf of California, México. Pac. Sci. 57: 141-145.
Gárate-Lizárraga, I., J.J. Bustillos-Guzmán, R. Alonso- Rodríguez & B. Luckas. 2004. Comparative paralytic shellfish toxin profiles in two marine bivalves during outbreaks of Gymnodinium catenatum (Dinophyceae) in the Gulf of California. Mar. Pollut. Bull. 48: 397-402.
Hasle, G.R. 1978. Using the inverted microscope, pp. 196. In A. Sournia (ed.). Phytoplankton manual. UNESCO, Paris.
Hummert, C., M. Ritscher, K. Reinhardt & B. Luckas. 1997. Analysis of the characteristic PSP profiles of Pyrodinium bahamense and several strains of Alexandrium by HPLC based on ion-pair chromatographic separation, post-column oxidation, and fluorescence detection. Chromatographia 45: 312-316.
Jaime, E., G. Gerts & B. Luckas. 2002. Accumulation and detoxification of paralytic shellfish poisoning in different shellfish exposed to a toxic Alexandrium fundyense strain. Abstract. X International Conference on Harmful Algae. St. Petersburg, Florida. 139 p.
Keen, A.M. 1971. Sea Shells of Tropical West America. Marine Mollusks from Baja California, México to Perú. Standford University, USA. pp. 1064.
Mancilla-Cabrera, E., C. Ramírez-Camarena & L. Muñoz- Cabrera. 2000. Primer registro de Gymnodinium catenatum Graham (Gymnodiniaceae) como causante de marea roja en la Bahía de Acapulco, Gro., México, pp. 85-86. In R. Ríos-Jara, E. Juárez-Carrillo, M. Pérez-Peña, E. López-Iriarte, E.G. Robles-Jarero, D.U. Hernández-Becerril & M. Silva-Briano (eds.). Estudios sobre el plancton marino en México y el Caribe, Sociedad Mexicana de Planctología y Universidad de Guadalajara, México.
Mee, L.D., M. Espinosa & G. Díaz. 1986. Paralytic shellfish poisoning with a Gymnodinium catenatum red tide on the Pacific coast of Mexico. Mar. Environ. Res. 19: 17-92.
Oshima, Y., M. Hirota, T. Yasumoto, G.M. Hallegraeff, S.I. Blackburn & D.A. Steffensen. 1989. Production of paralytic shellfish toxins by the dinoflagellate Alexandrium minutum. Halim from Australia. Bull. Jap. Soc. Sci. Fish. 55: 920-925.
Oshima, Y., K. Sugino, M. Hirota & T. Yasumoto.1990. Comparative studies on paralytic shellfish toxin profiles of dinoflagellates and bivalves, pp. 391-396. In E. Graneli, B. Sundstrom, L. Elder & D.M. Anderson (eds.). Toxic Marine Phytoplankton. Elsevier, New York.
Sierra-Beltrán, A.P., M.L. Morquecho-Escamilla, C. Lechuga-Devéze & J.L. Ochoa. 1996. PSP monitoring program at Baja California Sur, Mexico, pp. 105-108. In T. Yasumoto, Y. Oshima & Y. Fukuyo (eds.). Harmful and Toxic Algal Blooms. Intergovernmental Oceanographic Commission of UNESCO, Paris.
Steidinger, K.A. & K. Tangen. 1996. Dinoflagellates, pp. 387-584. In C. Thomas (ed.). Identifying Marine Diatoms and Dinoflagellates. Academic, San Diego.
Takatani, T., H. Akaeda, T. Kaku, M. Miyamoto, H. Mukai & T. Noguchi. 1998. Paralytic shellfish poison infestation to oyster Crassostrea gigas due to dinoflagellate Gymnodinium catenatum in Amakusa Islands, Kumamoto Prefecture. J. Food Hyg. Soc. Jap. 39: 292-295.
Yu, R., C. Hummert, B. Luckas, P.Y. Qian & M.J. Zhou. 1998. A modified HPLC method for analysis of PSP toxins in algae and shellfish from China. Chromatographia 48: 671-676.
Mackenzie, L. & T. Beauchamp. 2001. Gymnodinium catenatum in New Zealand: a new problem for public health and the shellfish industry (Downloaded: August 17th, 2004, http://www.cawthron.org.nz/Assets/Gcat.pdf).
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