Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

Activity against Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) of methanolic extracts of medicinal use plants in Mexico
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Trypanosoma cruzi
Haematoxylum brasiletto
medicinal plants
methanolic extracts.
Trypanosoma cruzi
Haematoxylum brasiletto
plantas medicinales
extractos metanólicos.

How to Cite

Pérez-Treviño, K. C., Galaviz, L., Iracheta-Villarreal, J. M., Lucero-Velasco, E. A., & Molina-Garza, Z. J. (2017). Activity against Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) of methanolic extracts of medicinal use plants in Mexico. Revista De Biología Tropical, 65(4), 1459–1469.


American trypanosomiasis is a potentially lethal disease caused by the hemoflagellate protozoan Trypanosoma cruzi. This neglected disease affects from 6 to 7 million people worldwide. Currently there are only two medicines to treat this disease: beznidazol and nifurtimox, both effective if they are administrated in the acute phase of infection, although their effectiveness fades away in the chronic phase; it also induces significant side effects. The aim of this study is to screen the trypanocidal activity of methanolic extracts from Hematoxilum brasiletto, Marrubium vulgare, Schinus molle, and Cympongon citratus, against T. cruzi epimastigotes, followed by the chromatographic separation, and identification of active compounds of the best candidate by colored chemical reactions; furthermore, it was also determined their cytotoxic effect in human lymphocytes and the brine shrimp Artemia salina. The extract of H. brasiletto showed the highest anti-T. cruzi activity with a inhibitory concentration (IC50) of 543 µg/mL; in descending order, it was followed by M. vulgare (IC50 = 647 µg/mL), S. molle (IC = 827 µg/mL) and finally, C. citratus (IC = 1 210 µg/mL). The chromatographic fraction Fr22 from H. brasiletto showed the best anti-T. cruzi effectivity (IC50 = 0.238 mg/mL), when compared to the other fraction or the whole extract, with no cytotoxic effect against human lymphocytes or A. salina. The active compounds were identified as tannins, quinones, flavonoids and sesquiterpenlactones. In conclusion, active compounds against T. cruzi were identified for the first time in H. brasiletto, with non-cytotoxic effects. The H. brasiletto extract, according to our results, could be used as an alternative treatment for the Chagas disease; however, additional studies will be necessary to test their activity and doses in a murine model, with the complete identification of the active compounds, on which we are investigating.
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Abdel‐Sattar, E., Maes, L., & Salama, M. M. (2010). In vitro activities of plant extracts from Saudi Arabia against malaria, leishmaniasis, sleeping sickness and Chagas disease. Phytotherapy Research, 24, 1322-1328.

Abe, F., Nagafuji, S., Yamauchi, T., Okabe, H., Maki, J., Higo, H., Akahane, H., Aguilar, A., Jiménez-Estrada, M., & Reyes-Chilpa, R. (2002). Trypanocidal constituents in plants. 1. Evaluation of some Mexican plants for their trypanocidal activity and active constituents in Guaco, roots of Aristolochia taliscana. Biological and Pharmaceutical Bulletin, 25, 1188-1191.

Al-Adhroey, A. H., Nor, Z. M., Al-Mekhlafi, H. M., & Mahmud, R. (2010). Median lethal dose, antimalarial activity, phytochemical screening and radical scavenging of methanolic Lenguas galanga rhizome extract. Molecules, 15, 8366-8376.

Bardai, S. E., Lyoussi, B., Wibo, M., & Morel, N. (2001). Pharmacological evidence ofhypotensive activity of Marrubium vulgare and Foeniculum vulgare in sponta-neously hypertensive rat. Clinical and Experimental Hypertension, 23, 329-343.

Boyum, A. (1984). Separation of lymphocytes, granulocytes, and monocytes from human blood using iodinated density gradient media. Methods in Enzymology, 108, 88-102.

Calvo, M. S. (2015). Tripanosomiasis americana o enfermedad de Chagas. Revista Médica de Costa Rica y Centroamerica, 71, 539-544.

Camou-Guerrero, A., Reyes-García, V., Martínez-Ramos, M., & Casas, A. (2008). Knowledge and use value of plant species in a Rarámuri community: a gender perspective for conservation. Human Ecology, 36, 259-272.

Cano de Terrones, T. (2014). Caracterización de una espirolactona sesquiterpénica α-metilénica obtenida de Ambrosia arborescens Miller y evaluación de su actividad biológica en Trypanosoma cruzi. Revista de la Sociedad Química del Perú, 80, 124-135.

Carrilero, B., Murcia, L., Martinez-Lage, L., & Segovia, M. (2011). Side effects of benznidazole treatment in a cohort of patients with Chagas disease in non-endemic country. Revista Española de Quimioterapia, 24, 123-26.

Chagas, C. (1909). Nova tripanozomiaze humana: estudos sobre a morfolojia e o ciclo evolutivo do Schizotrypanum cruzi n. gen., n. sp., ajente etiolojico de nova entidade morbida do homem. Memórias do Instituto Oswaldo Cruz, 1, 159-218.

Cho, Y. H., Lee, J. W., Woo, H. D., Lee, S., Kim, Y. J., Lee, Y., Shin, S., Joung, H., & Chung, H. W. (2016). Protective effect of onion extract on bleomycin-induced cytotoxicity and genotoxicity in human lymphocytes. International Journal of Environmental Research and Public Health, 13, 227.

Cunha, W. R., Martins, C., Ferreira, D. D., Crotti, A. E., Lopes, N. P., & Albuquerque, S. (2003). In vitro trypanocidal activity of triterpenes from Miconia species. Planta Medica, 69, 470-471.

Dang, Q. L., Kwon, H. R., Choi, Y. H., Choi, G. J., Jang, K. S., Park, M. S., Lim, C. H., Ngoc, L. H., & Kim, J. C. (2010). Nematicidal activity against Bursaphelenchus xylophilus ofisoobtusilactone a isolated from Persea americana. Nematology, 12, 247-253.

Déciga-Campos, M., Rivero-Cruz, I., Arriaga-Alba, M., Castañeda-Corral, G., Angeles-López, G. E., Navarrete, A., & Mata, R. (2007). Acute toxicity and mutagenic activity of Mexican plants used in traditional medicine. Journal of Ethnopharmacology, 110, 334-342.

dos Santos, P. F., Moreira, D. S., Baba, E. H., Volpe, C. M., Ruiz, J. C., Romanha, A. J., & Murta, S. M. (2016). Molecular characterization of lipoamide dehydrogenase gene in Trypanosoma cruzi populations susceptible and resistant to benznidazole. Experimental Parasitology, 170, 1-9.

Espitia-Baena, J. E., Robledo-Restrepo, S. M., Cuadrado-Cano, B., Duran-Sandoval, H. D. R., & Gómez-Estrada, H. A. (2014). Perfil fitoquímico, actividad anti-Leishmania, hemolítica y toxicológica de Cordia dentata Poir y Heliotropium indicum L. Revista Cubana de Plantas Medicinales, 19, 208-224.

Fabbro, D., Velazquez, E., Bizai, M. L., Denner, S., Olivera, V., Arias, E., Pravia, C., & Ruiz, A. M. (2013). Evaluation of the ELISA-F29 test as an early marker of therapeutic efficacy in adults with chronic Chagas disease. Revista do Instituto de Medicina Tropical de São Paulo, 55, 167-172.

Fernández-Calienes, A., Mendiola, J., Monzote, L., García, M., Sariego, I., Acuña, D., Ramón, L., & Gutiérrez, Y. (2009). Evaluación de la toxicidad de extractos de plantas cubanas con posible acción antiparasitaria utilizando larvas de Artemia salina L. Revista Cubana de Medicina Tropical, 61, 254-258.

Ferrero, A. A., González, J. W., & Chopa, C. S. (2006). Biological activity of Schinus molle on Triatoma infestans. Fitoterapia, 77, 381-383.

Galarraga, E., Luis, J. M. A., Rojas, L., Offer, A. C. M., & Dubois, M. A. L. (2014). Triterpenos y saponinas triterpénicas de Phytolacca icosandra y Phytolacca rugosa. Ciencia, 22, 53-66.

García-Huertas, P., Mejía-Jaramillo, A. M., González, L., & Triana-Chávez, O. (2017). Transcriptome and functional genomics reveal the participation of adenine fhosphoribosyl transferase in Trypanosoma cruzi resistance to benznidazole. Journal of Cellular Biochemistry, DOI 10.1002/jcb.25978

Günaydin, K., & Savci, S. (2005). Phytochemical studies on Ruta chalepensis (Lam.) Lamark. Natural Products Research, 19, 203-210.

Instituto Nacional de Estadística y Geografía (INEGI). (2015). Conociendo Nuevo León. Quinta edición. Recuperado de

Kohatsu, A. A., Silva, F. A., Francisco, A. I., Rimoldi, A., Silva, M. T., Vargas, M. D., & Cicarelli, R. M. (2016). Differential expression on mitochondrial tryparedoxin peroxidase (mTcTXNPx) in Trypanosoma cruzi after ferrocenyl diamine hydrochlorides treatments. The Brazilian Journal of Infectious Diseases.

Kpoviessi, S., Bero, J., Agbani, P., Gbaguidi, F., Kpadonou-Kpoviessi, B., Sinsin, B., Accrombessi, G., Frédérich, M., Moudachirou, M., & Quetin-Leclercq, J. (2014). Chemical composition, cytotoxicity and in vitro antitrypanosomal and antiplasmodial activity of the essential oils of four Cymbopogon species from Benin. Journal of Ethnopharmacology, 151, 652-659.

Matta, P. M., Gutierrez, F. R., Nascimento, M. S., Do-Valle, M. A., & Silva, J. S. (2012). Antiparasitical chemotherapy in Chagas' disease cardiomyopathy: current evidence. Tropical Medicine and International Health, 17, 1057-65.

Martínez-Flórez, S., González- Gallego, J., & Culebras, J. M. (2002). Los flavonoides: propiedades y acciones antioxidantes. Nutrición Hospitalaria, 17, 271-278.

Martino, V. S. 2012. Problemática sanitaria y social de la enfermedad de Chagas. Aporte de la medicina tradicional argentina. Dominguezia, 28, 29-37.

Mijares-Bullaín, M., Torres-Rodriguez, E., & Hermosilla-Espinosa, R. (2014). Tamizaje fitoquímico de los extractos de Faramea occidentalis (L.) A. Rich. (nabaco). Revista Cubana de Plantas Medicinales, 19, 421-432.

Mejía-Jaramillo, A. M., Fernández, G. J., Montilla, M., Nicholls, R. S., & Triana-Chávez, O. (2012). Sensibilidad al benzonidazol de cepas de Trypanosoma cruzi sugiere la circulación de cepas naturalmente resistentes en Colombia. Biomédica, 32, 196-205.

Molina-Garza, Z. J., Bazaldúa-Rodríguez, A. F., Quintanilla-Licea, R., & Galaviz-Silva, L. (2014). Anti-Trypanosoma cruzi activity of 10 medicinal plants used in northeast Mexico. Acta Tropica, 136, 14-18.

Monzote, L., Alarcón, O., & Setzer, W. N. (2012). Antiprotozoal activity of essential oils review. Agriculturae Conspectus Scientificus, 77, 167-175.

Muelas-Serrano, S., Nogal-Ruiz, J., & Gómez-Barrio, A. 2000. Setting of a colorimetric method to determine the viability of Trypanosoma cruzi epimastigotes. Parasitology Research 86, 999-1002.

Muñoz-Jáuregui, A. M., Ramos-Escudero, D. F., Alvarado-Ortiz Ureta, C., & Castañeda-Castañeda, B. (2007). Evaluación de la capacidad antioxidante y contenido de compuestos fenólicos en recursos vegetales promisorios. Revista de la Sociedad Química del Perú, 73, 142-149.

Murcia, L., Carrilero, B., Viñas, P. A., & Segovia, M. (2012) Nifurtimox chemotherapy: collateral effects in treated Trypanosoma cruzi infected patients. Revista Española de Quimioterapia, 25, 1-3.

Murillo, R., Castro, V., Chavarría, M., & Poveda, L. (2006). Lignanos de Zanthoxylum acuminatum. Revista de Ciencia y Tecnología, 24, 227-232.

Navarro, V., Villarreal, M. L., Rojas, G., & Lozoya, X. (1996). Antimicrobial evaluation of some plants used in Mexican traditional medicine for the treatment of infectious diseases. Journal of Ethnopharmacology, 53, 143-147.

Oketch-Rabah, H. A., Christensen, S. B., Frydenvang, K., Dossaji, S. F., Theander, T. G., Cornett, C., Watkins, W. M., Kharazmi, A., & Lemmich, E. (1998). Antiprotozoal properties of 16, 17-dihydrobrachycalyxolide from Vernonia brachycalyx. Planta Medica, 64, 559-562.

Ortega, N. S., Benitez-Bampo, N., & Cabezas-Fajardo, F. A. (2011). Antibacterial activity and cualitative composition propolis from two climatic regions Cauca department. Biotecnología en el Sector Agropecuario y Agroindustrial, 9, 8-16.

Paveto, C., Güida, M. C., Esteva, M. I., Martino, V., Coussio, J., Flawiá, M. M., & Torres, H. N. (2004). Anti-Trypanosoma cruzi activity of green tea (Camellia sinensis) catechins. Antimicrobial Agents and Chemotherapy, 48, 69-74.

Pérez-Treviño, K. C., Molina-Garza, Z. J., & Galaviz-Silva, L. (2016) Evaluación de la actividad anti-trypanosomal de extractos metanólicos de plantas con uso medicinal. Entomología Mexicana, 3, 656-659.

Pino-Pérez, O., & Jorge-Lazo, F. (2010). Ensayo de Artemia: útil herramienta de trabajo para ecotoxicólogos y químicos de productos naturales. Revista de Protección Vegetal, 25, 34-43.

Pizzolatti, M. G., Koga, A. H., Grisard, E. C., & Steindel, M. (2002). Trypanocidal activity of extracts from Brazilian Atlantic rain forest plant species. Phytomedicine, 10, 422-426.

Quintanilla-Licea, R., Morado-Castillo, R., Gomez-Flores, R., Laatsch, H., Verde-Star, M. J., Hernández-Martínez, H., Tamez-Guerra, P., Tamez-Guerra, R., & Rodríguez-Padilla, C. (2012). Bioassay-guided isolation and identification of cytotoxic compounds from Gymnosperma glutinosum leaves. Molecules, 17, 11229-11241.

Ramírez, M. E., Mendoza, J. A., Arreola, R. H., & Ordaz, C. (2010). Flavonoides con actividad antiprotozoaria. Revista Mexicana de Ciencias Farmacéuticas, 41, 6-21.

Rathore, S. K., Bhatt, S., Dhyani, S., & Jain, A. (2012). Preliminary phytochemical screening of medicinal plant Ziziphus mauritiana Lam. fruits. International Journal of Current Pharmaceutical Review and Research, 4, 160-162.

Rivero-Cruz, J. F. (2008). Antimicrobial compounds isolated from Haematoxylon brasiletto. Journal of Ethnopharmacology, 119, 99-103.

Rosas-Piñón, Y., Mejía, A., Díaz-Ruiz, G., Aguilar, M. S., Sánchez-Nieto, S., & Rivero-Cruz, J. F. (2012). Ethnobotanical survey and antibacterial activity of plants usedin the Altiplane region of Mexico for the treatment of oral cavity infections. Journal of Ethnopharmacology, 141, 860-865.

Sahpaz, S., Garbacki, N., Tits, M., & Bailleul, F. (2002). Isolation and pharmacological activity of phenylpropanoid esters from Marrubium vulgare. Journal of Ethnopharmacology, 79, 389-392.

Schlemper, B. R., Chiari, E., & Brener, Z. 1977. Growth-inhibition drug test with Trypanosoma cruzi culture forms. Journal of Protozology, 4, 544-547.

Slot, E., Hogema, B. M., Molier, M., Bart, A., & Zaaijer, H. L. (2016). Risk Factors and Screening for Trypanosoma cruzi Infection of Dutch Blood Donors. PloS one, 11(3), 1-9.

Sotomayor, R. (2014). Extracción y cuantificación de antocianinas a partir de los granos de Zea mays L. (maíz morado). Ciencia y Desarrollo, 16, 69-74.

Sülsen, V. P. (2012). Búsqueda de compuestos antiprotozoarios en especies de la flora medicinal argentina. Dominguezia, 28, 19-27.

Tomás-Alonso, F. (1993). Química orgánica estructural. Vol. 5 de Manuales y materiales didácticos. Murcia, España: EDITUM.

Vega-Menchaca, M. D. C., Verde-Star, J., Oranday-Cárdenas, A., Morales-Rubio, M. E., Núñez-González, M. A., Rivera-Guillén, M. A., Serrano-Gallardo, L. J., & Rivas-Morales, C. (2013). Actividad antibacteriana y citotóxica de Leucophyllum frutescens (Berl) IM Johnst del Norte de México contra Staphylococcus aureus de aislados clínicos. Revista Mexicana de Ciencias Farmacéuticas, 44, 24-30.

Villarreal, D., Barnabé, C., Sereno, D., & Tibayrenc, M. (2004). Lack of correlation between in vitro susceptibility to benznidazole and phylogenetic diversity of Trypanosoma cruzi, the agent of Chagas disease. Experimental Parasitology, 108, 24-31.

Volonté, M. G. & Quiroga, P. (2013). Análisis farmacéutico, Universidad Nacional de La Plata. Buenos Aires: Edulp.

Wang, X., Beno-Movalem, D., Kobiler, I., Leikin-Frenkel, A., Lichter, A., & Prusky, D. 2004. Expression of Δ12 fatty acid desaturase during the induced accumulation of the antifungal diene in avocado fruits. Molecular Plant Pathology, 5, 575-585.

Werner, A. B., & Zulantay, I. (2011). Estado actual en el tratamiento de la enfermedad de Chagas. Revista Médica de Chile, 139, 247-257.

World Health Organization (WHO). 2015. Chagas disease (American trypanosomiasis). Fact sheet N°340. Updated. March 2015.

Yasunaka, K., Abe, F., Nagayama, A., Okabe, H., Lozada-Perez, L., Lopez-Villafranco, E., Estrada Muñiz, E., Aguilar, A., & Reyes-Chilpa, R. (2005). Antibacterial activity of crude extracts from Mexican medicinal plants and purified coumarins and xanthones. Journal of Ethnopharmacology, 97, 293-299.

Yueqin, Z., Recio, M. C., Máñez, S., Giner, R. M., Cerdá-Nicolás, M., & Ríos, J. L. (2003). Isolation of two triterpenoids and a bioflavanone with anti-inflammatory activity from Schinus molle fruits. Planta Medica, 69, 893-898.



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