Keywords
estrogen
feces
estrous cycle
hematology
sloths
AIA-360
progesterona
estrógeno
heces
ciclo estral
hematología
perezosos
AIA-360
How to Cite
Abstract
In wild animal species, the use of non-invasive and non-stressful procedures to determine hormone profiles, such as fecal steroid measurements, has considerably increased the comprehension of their reproductive physiology. Since there is limited information related to the reproductive hormone profiles of the two-toed sloth, Choloepus hoffmani, a study was conducted in captive specimens at the “Sloth Sanctuary” (Cahuita, Limón, Costa Rica), in order to determine: (i) the reliability of the fecal progesterone and estrogen extraction and its quantification with an AIA-360® analyzer, (ii) assess blood parameters in this species and (iii) evaluate if there is a correlation between fecal and plasmatic steroids. The study was performed over a three-month period, from November, 2013 to January, 2014, with a total amount of 208 fecal samples collected from five sexually mature females weighing 6.32 Kg in average. The average of the median concentrations of progesterone in feces of the five females was 124.21 ng/g, and 1 708.95 pg/g for estrogen. The average minimal and maximal values were 50.96 ng/g and 1 057.46 ng/g for progesterone and, 1 191.77 pg/g and 2 159.24 pg/g for estradiol. In plasma, progesterone median values were 1.26 ng/mL, showing a minimum of 0.32 ng/mL and 12.84 ng/mL as maximum values. The plasmatic estrogen levels were below the detection limit of the equipment (25 pg/mL). Although there was no strong statistical correlation between the fecal and plasmatic progesterone fluctuations, our data suggests that the plasmatic events are mostly reflected in feces two days afterwards. Also, the levels of progesterone were elevated during the first half of November and, subsequently, showed a successive and important reduction in all the females tested. Finally, our results demonstrated that fecal steroid extractions and their measurement in a AIA-360®, allowed the successful detection and represents an alternative non-invasive determination of hormone profiles in C. hoffmani.
References
Adlercreutz, H., Höckerstedt, K., Bannwart, C., Bloigu, S., Hämäläinen, E., Fotsis, T., & Ollus, A. (1987). Effect of dietary components, including lignans and phytoestrogens, on enterohepatic circulation and liver metabolism of estrogens and on sex hormone binding globulin (SHBG). Journal of Steroid Biochemistry, 27, 1135-1144.
Araújo, G. (2006). Aspectos clínicos e de manejo de preguiça-de-garganta-marrom Bradypus variegatus (Schinz, 1825) de vida libre na Mesorregãio Metropolitana do Recife, Pernambuco, Brasil. (Tese ao Programa de Pós-graduação). Universidade Federal Rural de Pernambuco, Pernambuco, Brasil.
Brown, J., Walker, S., & Steinman, K. (2009). Endocrine manual for reproductive assessment of domestic and non-domestic species. Smithsonian's National Zoological Park. Washington, United States of America.
Chelini, M., Souza, N., Rocha, A., Felippe, E., & Oliveira C. (2005). Quantification of fecal estradiol and progesterone metabolites in Syrian hamsters (Mesocricetus auratus). Brazilian Journal of Medical and Biological Research, 38, 1711-1717.
Dias, B., dos-Santos, L., Lara, P., Righetto, C.. Pinder, L., & Chiarello A. (2007). First observation on mating and reproductive seasonality in maned sloths Bradypus torquatus (Pilosa: Bradypodidae). Journal of Ethology, 27, 97-103.
Dumonceaux, G., Bauman, J., & Carillo G. (2006). Evaluation of progesterone levels in feces of captive reticulated giraffe (Giraffa camelopardalis reticulata). Journal of Zoo and Wildlife Medicine, 37, 255-261.
Durán, A. 2005. Valores de hematología y de bioquímica sanguínea del perezoso de tres dedos (Bradypus variegatus) y el perezoso de dos dedos (Choloepus hoffmanni) en cautiverio en Limón, Costa Rica. (Tesis de Licenciatura). Universidad Nacional, Heredia, Costa Rica.
Gilmore, D., Da-Costa, D., & Duarte, D. (2000). An update on the physiology of two- and three-toed sloths. Brazilian Journal of Medical and Biological Research, 33, 129-146.
Goldin, B.R., Adlercreutz, H., Dwyer, J.T., Swenson, L., Warram, J. H., & Gorbach, SL. (1981). Effect of Diet on Excretion of Estrogens in Pre and Postmenopausal Women. Cancer Research, 41, 3771 -3773.
Goymann, W. (2005). Noninvasive monitoring of hormones in bird droppings: physiological validation, sampling, extraction, sex differences, and the influence of diet on hormone metabolite levels. Annals of the New York Academy of Sciences, 1046, 35-53.
Goymann, W. (2012). Review: On the use of non-invasive hormone research in uncontrolled, natural environments: the problem with sex, diet, metabolic rate and the individual. Methods in Ecology and Evolution, 3, 757-765.
Graham, L. (2004). Non-invasive monitoring of reproduction in zoo and wildlife species. Annual Review of Biomedical Sciences, 6, 91-98.
Graham, L., Schwarzenberger F., Möstl E., Galama, W., & Savage A. (2001). A versatile enzyme immunoassay for the determination of progestogens in feces and serum. Zoo Biology, 20, 227-236.
Guimarães-Martins, E., Bonato, V., Queiroz-da-Silva, C., & Furtado-dos-Reis, S. (2006). Seasonality in reproduction, age structure and density of the gracile mouse opossum Gracilinanus microtarsus (Marsupialia: Didelphidae) in a Brazilian cerrado. Journal of Tropical Ecology, 22, 461-468.
Hanley, C. (2008). Immobilization of free-ranging Hoffmann’s two-toed (Choloepus hoffmanni) and brown-throated three-toed (Bradypus variegatus) sloths using ketamine and medetomidine: a comparison of physiologic parameters. Journal of Wildlife Diseases, 44, 938-945.
Hayssen, V. (2011). Choloepus hoffmanni (Pilosa: Megalonychidae). Mammalian Species, 43, 37-55.
Keay, J., Singh J., Gaunt M., & Kaur T. (2006). Fecal glucocorticoids and their metabolites as indicators of stress in various mammalian species: a literature review. Journal of Zoo and Wildlife Medicine, 37, 234-244.
Kinney, M., Cole G., Vaughan, C., & Sladky, K. (2013). Physiologic and serum biochemistry values in free-ranging hoffmann's two-toed (Choloepus hoffmanni) and brown-throated three-toed (Bradypus variegatus) sloths immobilized using dexmedetomidine and ketamine. Journal of Zoo and Wildlife Medicine, 44, 570-580.
Mair, T.S. (2013). Equine medicine, surgery and reproduction. London, England: W.B. Saunders.
Meneses-Guevara, A. & Bouza-Mora L.S. (2014). Manual de hematología y química clínica en medicina veterinaria. Heredia, Costa Rica: EUNA.
Miller, R.E., & Fowler, ME. (2015). Fowler's Zoo and Wild Animal Medicine. Volume 8. St Louis, Missouri: Elsevier Saunders.
MINAET (Ministerio de Ambiente, Energía y Telecomunicaciones) & IMN (Instituto Meteorológico Nacional de Costa Rica). (2009). Segunda Comunicación Nacional a la Convención Marco de la Naciones Unidas sobre Cambio Climático. San José, Costa Rica.
Mühlbauer, M., Duarte, D., Gilmore, D.P., & Da-Costa, C. (2006). Fecal estradiol and progesterone metabolite levels in the three-toed sloth (Bradypus variegatus). Brazilian Journal of Medical and Biological Research, 39, 289-295.
Palme, R. (2005). Measuring fecal steroids: guidelines for practical applications. Annals of the New York Academy of Sciences, 1046, 75-80.
Paris, M., Whiteb, A., Reissb, A., Westa, M., & Schwarzenberger F. (2002). Faecal progesterone metabolites and behavioural observations for the non-invasive assessment of oestrous cycles in the common wombat (Vombatus ursinus) and the southern hairy-nosed wombat (Lasiorhinus latifrons). Animal Reproduction Science, 72, 245-257.
Pereira, R., Barbanti, J., & Negrão J. (2005). Seasonal changes in fecal testosterone concentrations and their relationship to the reproductive behavior, antler cycle and grouping patterns in free-ranging male Pampas deer (Ozotoceros bezoarticus bezoarticus). Theriogenology, 63, 2113-2125.
Porras, A., Zarco, L., & Valencia, J. (2004). Estacionalidad reproductiva en ovejas. Ciencia Veterinaria, 9, 1-33.
R Core Team. (2014). R: A language and environment for statistical computing. R Foundation for Statistical Computing (versión 3.3.1) [Software]. Vienna, Austria: The R Project for Statistical Computing. Recuperado de http://www.R-project.org/ (Consulta: 25 marzo, 2015).
Reece, W. O. (Ed). (2009). Dukes fisiología de los animales domésticos. 1.ed. Zaragoza, España: Acribia.
Schwarzenberger, F. (2007). The many uses of non-invasive faecal steroid monitoring in zoo and wildlife species. International Zoo Yearbook, 41, 52-74.
Sherman, R.A. (2015). Multicon: Multivariate Constructs. R package (versión 1.6) [Software]. Vienna, Austria: The R Project for Statistical Computing. Recuperado de http://CRAN.R-project.org/package=multicon (Consulta: 7 de julio, 2017).
Signorell, A., Aho, K., Alfons, A., Anderegg, N., Aragon, T., … & Zeileis. A. (2017). DescTools: Tools for descriptive statistics. R package (versión 0.99.21) [Software]. Vienna, Austria: The R Project for Statistical Computing. Recuperado de http://CRAN.R-project.org/package=DescTools (Consulta: 7 de julio, 2017).
Snoeck, P.P.N., Cruz, A.C.B., Catenacci, L.S., & Cassano, C.R. (2011). Citologia vaginal de preguiça-de-coleira (Bradypus torquatus). Pesquisa Veterinária Brasileira, 31, 271-275.
Suárez-Esquivel, M., & Castro-Ramírez, L. (2016). Measurement of thyroid hormones and cortisol in horses with an automated immunoassay analyzer. Revista Ciencias Veterinarias, 34(1), 39-49. DOI: http://dx.doi.org/10.15359/rcv.34-1.3
Taube, E., Keravec J., Vié J., & Duplantier J. (2001). Reproductive biology and postnatal development in sloths, Bradypus and Choloepus: review with original data form the field (French Guiana) and from captivity. Mammal Review, 31, 173-188.
Touma, C., & Palme R. (2005). Measuring fecal glucocorticoid metabolites in mammals and birds: the importance of validation. Annals of the New York Academy of Sciences, 1046, 54-74.
Troll, S., Gottschalk, J., Seeburger, J., Ziemssen, E., Häfner, M., Thielebein, J., & Einspanier, A. (2013). Characterization of the ovarian cycle in the two-toed sloths (Choloepus didactylus): an innovative, reliable, and noninvasive method using fecal hormone analyses. Theriogenology, 80, 275-283.
Valdespino, C., Martínez-Mota, R., García-Feria, L.M., & Martínez-Romero L.E. (2007). Evaluación de eventos reproductivos y estrés fisiológico en vertebrados silvestres a partir de sus excretas: evolución de una metodología no invasiva. Acta Zoológica Mexicana, 23, 151-180.
Vogel, I., Vie, J., Thoisy, B., & Moreau B. (1999). Hematological and serum chemistry profiles of free-ranging southern two-toed sloths in French Guiana. Journal of Wildlife Diseases, 35, 531-535.
Wainwright, M. (2007). The mammals of Costa Rica: a natural history and field guide. 1.ed. New York, USA: Comstock Publishing Associates.
Wasser, S. K., Thomas, R., Nair, P. P., Guidry, C., Southers, J., Lucas, J., Wildt, D. E., & Monfort S. L. (1993). Effects of dietary fibre on faecal steroid measurements in baboons (Papio cynocephalus cynocephalus). Journal of Reproduction and Fertility, 97, 569-574.
Wickham, H. (2009). Ggplot2: elegant graphics for data analysis. New York, USA: Springer.
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