Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://revistas.ucr.ac.cr/index.php/rbt/oai
Immuno-gold replica technique for ultrastructural localization of antigens
Vol. 42 No. S2 (1994), Articles
Vol. 42 No. S2 (1994)

Immuno-gold replica technique for ultrastructural localization of antigens

Articles
Published August 1, 1994
Francisco Hernández
Universidad de Costa Rica
Patricia Rivera
Hospital Nacional de Niños
Yasuhiro Hosaka
Osaka University of Pharmaceutical Sciences
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Keywords

Sendai virus
immunolabelling
immunoelectronmicroscopy
replica
ultrastructure

How to Cite

Hernández, F., Rivera, P., & Hosaka, Y. (1994). Immuno-gold replica technique for ultrastructural localization of antigens. Revista De Biología Tropical, 42(S2), 179–182. Retrieved from https://revistas.ucr.ac.cr/index.php/rbt/article/view/28717
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Abstract

We developed a simple method for immunolabelling viral glycoproteins on surface of infected ceil monolayers. Cells 9 hours post-infected with Sendai virus were fixed and treated with polyclonal antibody anti HN glycoprotein (Rabbit IgG), labelled with goat immunoglobulin anti rabbit IgG conjugated with colloidal gold particles. Then, the cells were shadowed with platinum-carbon, covered with nitrocellulose glue, and digested with domestic bleach, washed, and replica fragments were caught with uncovered grid.
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Copyright (c) 1994 Revista de Biología Tropical

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