Tokophrya quadripartita (Claparede & Lachmann,1861) Bütschli, 1889 is reported in Mexico for the first time, from stagnant waters of Chapultepec Park, in Mexico City. The protozoan was found associated with Epistylis plicatilis Ehremberg, 1838 and on shells of Limnaea attenuata Say and Physa osculans Aldeman. The morphology and biology of the microorganism are described.