de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

Cellulolytic ability of a promising Irpex lacteus (Basidiomycota: Polyporales) strain from the subtropical rainforest of Misiones province, Argentina

Ernesto Martin Giorgio, Laura Lidia Villalba, Gerardo Lucio Robledo, Pedro Dario Zapata, Mario Carlos Saparrat



The cellulolytic activity of fungi growing in the subtropical rainforest of Misiones (Argentina) represents a challenge in the technological development of the production of cellulosic bioethanol in the region using native sources. These fungi are promising to obtain sustainable enzyme cocktails using their enzymes. Cellulolytic ability of 22 white-rot fungi isolated from the subtropical rainforest of Misiones-Argentina in agar medium with two types of cellulosic substrates, carboxy-methylcellulose or crystalline cellulose, were comparatively analyzed, and the activity of two cellulolytic enzymes was evaluated in liquid medium. Although all isolates were able to grow and degrade both substrates in agar medium, and to produce total cellulase Filter paper (FPase) and endo-β-1,4-glucanase (EG) activities in broth, the isolate Irpex sp. LBM 034 showed the greatest enzymatic levels (FPase, 65.45 U L-1; EG, 221.21 U L-1). Therefore, the ITS sequence of this fungus was sequenced and analyzed through a phylogenetic analysis. These results indicate that the isolate LBM 034, corresponding to Irpex lacteus, has a promising cellulolytic ability and enzymes such as EG useful in sustainable saccharification of cellulosic materials in the region.


White-rot fungi; Irpex lacteus;, cellulases; FPase; endo-β-1,4-glucanase


Akiba, S., Kimura, Y., Yamamoto, K., & Kumagai, H. (1995). Purification and characterization of a protease-resistant cellulase from Aspergillus niger. Journal of Fermentation and Bioengineering, 79(2), 125-130.

Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Research, 25(17), 3389-3402.

Andrino, A., Morte, A., & Honrubia, M. (2011). Caracterización y cultivo de tres cepas de Pleurotus eryngii (Fries) Quélet sobre sustratos basados en residuos agro-alimentarios. Anales de Biología, 33, 53-66.

Brown, A. D., Grau, A., Lomáscolo, T., & Gasparri, N. I. (2002). Una estrategia de conservación para las selvas subtropicales de montaña (Yungas) de Argentina. Ecotrópicos, 15(2), 147-159.

Cabrera, A. L. (1994). Enciclopedia Argentina de Agricultura y Jardinería. Fascículo 1. Regiones Fitogeográficas Argentinas. Buenos Aires: Editorial Acme.

Daâssi, D., Zouari-Mechichi, H., Belbahri, L., Barriuso, J., Martínez, M. J., Nasri, M., & Mechichi, T. (2016). Phylogenetic and metabolic diversity of Tunisian forest wood-degrading fungi: a wealth of novelties and opportunities for biotechnology. Biotechnology, 6(46), 1-16.

Fonseca, M. I., Tejerina, M. R., Sawostjanik-Afanasiuk, S. S., Giorgio, E. M., Barchuk, M. L., Villalba, L. L., & Zapata, P. D. (2016). Preliminary studies of new strains of Trametes sp. from Argentina for laccase production ability. Brazilian Journal of Microbiology, 47, 287-287.

Gallo, M. C., Robledo, G., Romero, A. I., & Catania, M. (2014). New records of Ascomycota in the Northwestern Argentinean Yungas. Checklist, 10(3), 621-631.

Ganner, T., Bubner, P., Eibinger M., Mayrhofer, C., Plank, H., & Nidetzky, B. (2012). Dissecting and reconstructing synergism in situ visualization of cooperativity among cellulases. Journal of Biological Chemistry, 287(52), 43215-43222.

Gao, Y., & Breuil, C. (1995). Extracellular lipase production by a sapwood-staining fungus Ophiostoma piceae. World Journal of Microbiology and Biotechnology, 11, 638-642.

Ghose, T. (1987). Measurement of cellulase activities. Pure and Applied Chemistry, 59, 257-268.

Giorgio, E. M., Fonseca, M. I., Tejerina, M. R., Ramos-Hryb, A. B., Sanabria, N., Zapata, P. D., & Villalba, L. L. (2012). Chips and sawdust substrates application for lignocellulolytic enzymes production by solid state fermentation. International Research Journal of Biotechnology, 3(7), 120-127.

Goloboff, P. A., Farris, J. S., & Nixon, K. C. (2008). TNT, a free program for phylogenetic analysis. Cladistics, 24, 774-786.

Guigón-López, C., Guerrero-Prieto, V., Vargas-Albores, F., Carvajal-Millán, E., Ávila-Quezada, G. D., Bravo-Luna, L., … Lorito, M. (2010). Identificación molecular de cepas nativas de Trichoderma spp., su tasa de crecimiento in vitro y antagonismo. Revista Mexicana de Fitopatología, 28(2), 87-96.

Habibi, Y., Lucia, L. A., & Rojas, O. J. (2010). Cellulose nanocrystals: Chemistry, selfassembly, and applications. Chemical Reviews, 110(6), 3479-3500.

Hamada, N., Ishikawa, K., Fuse, N., Kodaira, R., Shimosaka, M., Amano, Y., Kanda, T., & Okazaki, M. (1999). Purification, characterization and gene analysis of exo-cellulase II (Ex-2) from the white rot basidiomycete Irpex lacteus. Journal of Bioscience and Bioengineering, 87, 442-451.

Hamada, N., Kodaira, R., Nogawa, M., Shinji, K., Ito, R., Amano, Y., … Okazaki, M. (2001). Role of cellulose binding domain of exocellulase I from white rot basidiomycete Irpex lacteus. Journal of Bioscience and Bioengineering, 91, 359-362.

Hilden, K. S., Bortfeldt, R., Hofrichter, M., Hatakka, A., & Lundell, T. K. (2008). Molecular characterization of the basidiomycete isolate Nematoloma frowardii b19 and its manganese peroxidase places the fungus in the corticioid genus Phlebia. Microbiology, 154, 2371-2379.

Janusz, G., Rogalski, J., Barwińska, M., & Szczodrak, J. (2006). Effects of culture conditions on production of extracellular laccase by Rhizoctonia praticola. Polish Journal of Microbiology, 55, 309-19.

King, B., Donnelly, M., Bergstrom, G., Walker, L. P., & Gibson, D. (2008). An optimized microplate assay system for quantitative evaluation of plant cell wall degrading enzyme activity of fungal culture extracts. Biotechnology and Bioengineering, 102, 1033-1043.

Klein‐Marcuschamer, D., Oleskowicz‐Popiel, P., Simmons, B. A., & Blanch, H. W. (2012). The challenge of enzyme cost in the production of lignocellulosic biofuels. Biotechnology and Bioengineering, 109, 1083-1087.

Kudanga, T., & Mwenje, E. (2005). Extracellular cellulase production by tropical isolates of Aureobasidium pullulans. Canadian Journal of Microbiology, 51, 773-776.

Li, C., Yang, Z., Zhang, R. H. C., Zhang, D., Chen, S., & Ma, L. (2013). Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis, Journal of Biotechnology, 168(4), 470-477.

Llacza-Ladera, H. F. (2012). Evaluación de la actividad celulolítica del complejo enzimático celulasa en cepas fúngicas de los departamentos de Cajamarca, Lima, Junín, Huánuco. (Tesis para optar al Título Profesional de Biólogo - Microbiólogo – Parasitólogo). Facultad de Ciencias Biológicas. E.A.P. de Microbiología y Parasitología. Universidad Nacional Mayor de San Marcos.

Lynd, L. R., Weimer, P. J., van Zyl, W. H., & Pretorius, I. S. (2002). Microbial cellulose utilization: fundamentals and biotechnology. Microbiology and Molecular Biology Reviews, 66(3), 506-577.

Mangelli, P., & Forchiassin, F. (1999). Regulation of the cellulase complex production by Saccobolus saccaboloides, induction and repression by carbohydrates. Mycologia, 91(2), 359-364.

Martínez, A. E., Chiocchio, V. M., & Godeas, A. M. (2001). Hyphomycetes celulíticos en suelos de bosques de Nothofagus, Tierra del Fuego. Gayana Botánica, 58(2), 123-132.

Miller, G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31, 426-428.

Niveiro, N., Popoff, O. F., & Albertó, E. O. (2010). Contribución al conocimiento de los Agaricales s.l. de la Selva Paraneaense Argentina. Boletín de la Sociedad Argentina de Botánica, 45(1-2), 17-27.

Novotný, Č., Cajthaml, T., Svobodová, K., Šušla, M., & Šašek, V. (2009). White-rot fungus with biotechnological potential – review. Folia Microbiologica, 54(5), 375-390.

Nwodo-Chinedu, S., Okochi, V. I., Smith, H. A., & Omijidi, O. (2005). Isolation of cellulolytic microfungi involved in wood-waste decomposition: prospects for enzymatic hydrolysis of cellulosic wastes. International Journal of Biomedical and Health Sciences, 1(2), 41-45.

Ohnishi, K., Yoshida, Y., & Sekiguchi, J. (1994). Lipase production of Aspergillus oryzae. Journal of Fermentation and Bioengineering, 77, 490-495.

Rajchenberg, M., & Robledo, G. (2013). Pathogenic Polypores in Argentina. Forest Pathology, 43(3), 171-184.

Robledo, G., Giorgio, E. M., Franco, C. R. P., Popoff, O., & Decock, C. (2014). Gyrodontium sacchari (Spreng.) Hjortstam (Boletales, Basidiomycota) in America: new records and its geographic distribution. Checklist, 10(6), 1514-1519.

Saparrat, M. C. N., Martínez, M. J., Cabello, M. N., & Arambarri, A. M. (2002). Screening for ligninolytic enzymes in autochthonous fungal strains from Argentina isolated from different substrata. Revista Iberoamericana de Micología, 19, 181-185.

Shanmugam, P., Mani, M., & Narayanasamy, M. (2008). Biosynthesis of cellulolytic enzymes by Trichothecium roseum with citric acid mediated induction. African Journal of Biotechnology, 7(21), 3917-3921.

Siddiqui, K. S., Shemsi, A. M., Anwar, M. A., Rashid, M. H., & Rajoka, M. I. (1999). Partial and complete alteration of surface charges of carboxy-methylcellulase by chemical modification: thermostabilization in water-miscible organic solvent. Enzyme and Microbial Technology, 24(8-9), 599-608.

Sistema Argentino de Información Jurídica. (2012). Decreto reglamentario de la ley XVI - NRO. 106 que instituye el marco regulatorio de los recursos dendroenergéticos renovables en el ámbito de la provincia de Misiones. Recuperado de

Siqueira, G., Bras, J., & Dufresne, A. (2010). Cellulosic bionanocomposites: A review of preparation, properties and applications. Polymers, 2(4), 728-765.

Stephanopoulos, G. (2007). Challenges in engineering microbes for biofuels production. Science, 315, 801-804.

Tekere, M., Mswaka, A. Y., Zvauya, R., & Read, J. S. (2001). Growth, dye degradation and ligninolytic activity studies on Zimbabwean white rot fungi. Enzyme and Microbial Technology, 28(4-5), 420-426.

Teza, V. G., Fonseca, M. I., Walantus, L. H., Davalos, P., Toro, A. A, Cariaga-Martinez, A. … Zapata, P. D. (2012). Estandarización de marcadores moleculares microsatélites para su uso en la industria forestal de Misiones, Argentina. Revista Colombiana de Biotecnología, 14(1), 216-223.

Vilches, P. L. (2002). Determinación de la actividad de exoglucanasas de cepas fúngicas nativas de las provincias de Huaylas y Huaraz. (Tesis de grado) Facultad de Ciencias Biológicas. Universidad Nacional Mayor de San Marcos. Lima, Perú.

White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sminsky, & T. J. White (Eds.). PCR Protocols: A guide to methods and applications (315-322). New York, USA: Academic Press, Inc.

Wright, J. E., Lechner, B. E., & Popoff, O. F. (2008). Atlas pictórico del Parque Nacional Iguazú. Buenos Aires: L.O.L.A.

Zuloaga, F. O., Morrone, O., & Rodríguez, D. (1999). Análisis de la biodiversidad en plantas vasculares de la Argentina. Kurtziana, 27(1), 17-167.


  • There are currently no refbacks.

© 2017 Universidad de Costa Rica. Para ver más detalles sobre la distribución de los artículos en este sitio visite el aviso legal. Este sitio es desarrollado por UCRIndex y Open Journal Systems. ¿Desea cosechar nuestros metadatos? dirección OAI-PMH: