Knowledge of spatial patterns and interactions of tree species allows for understanding the ecological processes of spatiotemporal structures of tropical forests, becoming essential for the establishment of strategies for the conservation and management of their resources in long term. The aim of this study was to investigate spatial patterns and interactions of Astronium lecointei, Dinizia excelsa and Peltogyne paniculata, three dominant timber tree species in Jamari National Forest, Brazilian Amazon. Kernel estimator was used aiming to verify the possible influence of first-order factors on species distributions and Inhomogeneous K-function was applied to analyze spatial patterns and interactions of the species by means of second-order factors. Univariate analyses revealed different scale-dependent spatial patterns for the species. Aggregation related to ecological characteristics, such as preferential habitat and dispersal limitation, was verified for A. lecointei and P. paniculata. D. excelsa presented a random spatial pattern, explained by specific features of its establishment, such as the need for clearings due to light requirement. Interspecific associations were evidenced by bivariate analyses, in which the spatial attraction of the species resulted from the same preference for microhabitats and the repulsion was a result of niche segregation.