Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

Labial palps of Prestonia clarki (Lepidoptera, Pieridae): ultrastructure of sensilla and sensory organs
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scanning electron microscope (SEM).
microscopio electrónico de barrido (MEB)

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Vargas-Fernández, I., Castro Gerardino, D. J., & Llorente-Bousquets, J. E. (2018). Labial palps of Prestonia clarki (Lepidoptera, Pieridae): ultrastructure of sensilla and sensory organs. Revista De Biología Tropical, 66(3), 1324–1346.


Research on labial palps of Lepidoptera is scarce and requires the use of Scanning Electron Microscopy techniques. We describe and illustrate the three segments composing the labial palps of the Coliadinae butterfly Prestonia clarki, as well as the distribution of sensilla and both of palp's distinctive structures: the Reuter's sensitive patch and the Palp pit organ; in the palps, the basal segment is the longest, and the distal segment is the shortest. We found chaetic sensilla type 1 on the lateral outer surface of the segments. In the Reuter's sensitive patch and the Palp pit organ, sensilla are specialized. In a lateral internal view of the proximal section of the basal segment, Reuter's sensitive patch looks as an elongated area with hundreds of microtrichia, differentiated into three sections; the adjacent area shows fewer microtrichia of shorter length. In a longitudinal view of the distal segment, at the apex of the segment, is the bottle-shaped Palp pit organ; inside the Palp pit are coeloconic sensilla in the basal section and microtrichia in the distal one. In addition, two indeterminate types of sensilla similar to chaetic ones were found within the Palp pit; which we called chaetic sensilla types 2 and 3; chaetic sensilla type 1 are located on the edge of the Palp pit. The sensilla types and their distribution in the Palp pit agree with those described in other species of Lepidoptera.
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Altner, H., Sass, H., & Altner, I. (1977). Relationship between structure and function of antennal chemo-, hygro-, and thermoreceptive sensilla in Periplaneta americana. Cell and Tissue Research, 176(3), 389-405.

Barcaba, T., & Krenn, H. W. (2015). The mouthparts of adult Indian meal moths, Plodia interpunctella (Hübner, 1813) (Lepidoptera: Pyralidae). Entomologica Austriaca, 22, 91-105.

Bilotta, I. G. (1992). Morfologia comparada da cabeça das espécies sulbrasileiras de Morphinae (Lepidoptera, Nymphalidae). Revista Brasileira de Zoologia, 9(3/4), 261-271.

Bogner, F., Boppré, M., Ernst, K. D., & Boeckh, J. (1986). C02 sensitive receptors on labial palps of Rhodogastria moths (Lepidoptera: Arctiidae): physiology, fine structure and central projection. Journal of Comparative Physiology A, 158, 741-749

Bogner, F. (1990). Sensory physiological investigation of carbon dioxide receptors in Lepidoptera. Journal of insect Physiology, 36(12), 951-957.

Bozzola J. J., & Russell, L. D. (1992). Specimen preparation for Scanning Electron Microscopy. In J. J. Bozzola (Ed.), Electron Microscopy: Principles and Techniques for Biologists (2 Ed., pp. 50-71). Massachusetts, EUA: Jones and Bartlett Publishers.

Braby, M. F., Vila, R., & Pierce, N. E. (2006). Molecular phylogeny and systematics of the Pieridae (Lepidoptera: Papilionoidea): higher classification and biogeography. Zoological Journal of the Linnean Society, 147, 239-275.

Chen, J., & Hua, B. (2016). Sexual dimorphism of adult labial palps of the peach fruit moth Carposina sasakii Matsumura (Lepidoptera: Carposinidae) with notes on their sensilla. Acta Zoologica (Stockholm), 97, 42-48.

DeVries, P. J. (1979). Pollen-feeding rainforest Parides and Battus butterflies in Costa Rica. Biotropica, 11(3), 237-238.

DeVries, P. J. (1987). The butterflies of Costa Rica and their natural history. Papilionidae, Pieridae and Nymphalidae (Vol. 1). New Jersey, EUA: Princeton University Press.

Dias, F. M. S., Casagrande, M. M., & Mielke, O. H. H. (2010). Morfologia do exoesqueleto de adultos de Memphis moruus stheno (Pritwittz) (Lepidoptera, Nymphalidae, Charaxinae). Revista Brasileira de Entomologia, 54(3), 376-398.

Dong, J. F., Liu, H., Tang, Q. B., Liu, Y., Zhao, X. C., & Wang, G. R. (2014). Morphology, type and distribution of the labial-palp pit organ and its sensilla in the oriental armyworm, Mythimna separata (Lepidoptera: Noctuidae.) Acta Entomologica Sinica, 57(6), 681-687. (En Chino, abstract en inglés)

Duarte, M., Casagrande, M. M., & Mielke, O. H. H. (2001). Morfologia externa do adulto de Hemiargus hanno (Stoll) (Lepidoptera, Lycaenidae, Polyommatinae, Polyommatini). I. Cabeça. Revista Brasileira de Zoologia, 18(1), 225-238.

Faucheux, M. J. (1999). Biodiversité et unité des organes sensoriels des insectes lépidoptères. Bulletin de la Societé des Sciences Naturelles del´Ouest de la France, Supplemént Hors Série, 1-296.

Faucheux, M. J. (2008). Mouthparts and associated sensilla of a South American moth, Synempora andesae (Lepidoptera: Neopseustidae). Revista de la Sociedad Entomológica Argentina, 67(1-2), 21-33.

Faucheux, M. J. (2013). Sensillum types on the proboscis of the Lepidoptera: a review, Annales de la Société entomologique de France (N. S.), 49(1), 73-90.

Faucheux, M. J., Kristensen, N. P., & Yen, S. H. (2006). The antennae of neopseustid moths: Morphology and phylogenetic implications, with special reference to the sensilla (Insecta, Lepidoptera, Neopseustidae). Zoologischer Anzeiger, 245, 131-142.

Hallberg, E., Hansson, B. S., & Löfstedt, C. (2003). Sensilla and proprioceptors. In N. P. Kristensen (Ed.), Lepidoptera, Moths and Butterflies. Morphology, Physiology and Development (Vol. 2, pp. 267-288). Berlin, New-York: Walter de Gruyter.

Hernández, B. C., Flores-Gallardo, A., & Llorente-Bousquets, J. (2014). Morfología del corion en la subfamilia Coliadinae (Lepidoptera: Pieridae). Southwestern Entomologist, 39(4), 853-885.

Kawahara, A. Y., Ortiz-Acevedo, E., & Marshall, C. J. (2012). External morphology of adult Libythea celtis (Laicharting [1782]) (Lepidoptera: Nymphalidae). Zoological Science, 29, 463-476.

Keil, T. A. (1996). Sensilla on the maxillary palps of Helicoverpa armigera caterpillars: in search of the CO2-receptor. Tissue Cell, 28, 703-717.

Kent, K. S., Harrow, I. D., Quartararo, P., & Hildebrand, J. G. (1986). An accessory olfactory pathway in Lepidoptera: the labial pit organ and its central projections in Manduca sexta and certain other sphinx moths. Cell and Tissue Research, 245, 237-245.

Klots, A. B. (1931-1933). A generic revision of the Pieridae (Lepidoptera). Together with a study of the male genitalia. Entomologica americana, 12(3), 139-204; (4), 205-242.

Krenn, H. W. (2010). Feeding mechanisms of adult Lepidoptera: Structure, function, and evolution of the mouthparts. Annual Review of Entomology, 55, 307-327.

Krenn, H. W., & Penz, C. M. (1998). Mouth parts of Heliconius butterflies (Lepidoptera: Nymphalidae): A search for anatomical adaptations to pollen-feeding behavior. International Journal of Insect Morphology and Embriology, 27(4), 301-309.

Kristensen, N. P., Scoble, M. J., & Karsholt, O. (2007). Lepidoptera phylogeny and systematics: the state of inventorying moth and butterfly diversity. Zootaxa, 1668, 699-747.

Lamas, G. (2004). Pieridae. Checklist: Part 4A. Hesperioidea - Papilionoidea. In J. B. Heppner (Ed.), Atlas of Neotropical Lepidoptera (Vol. 5A, pp. 99-117). Association for Tropical Lepidoptera. Gainesville, EUA: Scientific Publishers.

Lee, J. K. (1987). Development of the hair mechanosensilla on the pupal labial palp of the butterfly, Pieris rapae L. (Lepidoptera: Pieridae.). International Journal of Insect Morphology and Embriology, 16(5/6), 343-354.

Lee, J. K., & Altner, H. (1986a). Primary sensory projections of the labial palp-pit organ of Pieris rapae L. (Lepidoptera - Pieridae). International Journal of Insect Morphology and Embriology, 15(5/6), 439-448.

Lee, J. K., & Altner, H. (1986b). Structure, development and death of sensory cells and neurons in the pupal labial palp of the butterflies Pieris rapae L. and Pieris brassicae L. (Insecta, Lepidoptera). Cell Tissue Research, 244, 371-383.

Lee, J. K., Altner, H., Kim, W. K., & Kim, C. W. (1989). Axonal pathfinding in developing labial palps of the butterflies, Pieris rapae and Pieris brassicae. Cell Tissue Research, 256, 87-93.

Lee, J. K., Kim, C. W., & Altner, H. (1988). Differences in degeneration of the apical scolopidial organ in the labial palp of Lepidoptera during pupal development. Zoomorphology, 108, 77-83.

Lee, J. K., Seizer, R., & Altner, H. (1985). Lamellated outer dendritic segments of a chemoreceptor within wall-pore sensila in the labial palp-pit organ of the butterfly, Pieris rapae L. (Insecta, Lepidoptera). Cell Tissue Research, 240, 333-342.

Leite, L. A. R., Casagrande, M. M., & Mielke, O. H. H. (2010). External morphology of the adult of Heraclides anchisiades capys (Hübner, [1809]) (Lepidoptera – Papilionidae) I. Head, cephalic appendages and cervical region. Brazilian Archives of Biology and Technology, 53(5), 1119-1126.

Ljungberg, H., & Hallberg, E. (1992). Ultrastructure and distribution patterns of sensilla on the palps of Caddisflies (Trichoptera). International Journal of Insect Morphology and Embriology, 21(4), 337-346.

Luis-Martínez, M. A., Llorente-Bousquets, J., & Vargas-Fernández, I. (2005). Una megabase de datos de mariposas de México y la regionalización biogeográfica. En J. Llorente-Bousquets, & J. J. Morrone (Eds.), Regionalización biogeográfica en Iberoamérica y tópicos afines: Primeras Jornadas Biogeográficas RIBES (pp. 269-294). Ciudad de México: Las Prensas Ciencias, Facultad de Ciencias, UNAM.

Llorente-Bousquets, J., Luis-Martínez, M. A., & Vargas-Fernández, I. (2006). Apéndice general de Papilionoidea: Lista sistemática, distribución estatal y provincias biogeográficas. En J. J. Morrone, & J. Llorente-Bousquets (Eds.), Componentes bióticos principales de la entomofauna mexicana, (Vol. II, pp. 945-1009). Ciudad de México: Las Prensas Ciencias, Facultad de Ciencias, UNAM.

Ma, M., Chang, M. M., Lu, Y., Lei, C. L., & Yang, F. L. (2016). Ultrastructure of sensilla of antennae and ovipositor of Sitotroga cerealella (Lepidoptera: Gelechiidae), and location of female sex pheromone gland. Scientific Reports, 7, 40637. doi: 10.1038/srep40637

Maza, J. (1975). Una nueva forma de Prestonia clarki Schaus (Pieridae). Revista de la Sociedad mexicana de Lepidopterología, 1(2), 38-41.

Mielke, C. G. C., Mielke, O. H. H., & Casagrande, M. M. (2004). Estudo comparado da morfologia externa de Zaretis itys itylus (Westwood) e Agrias claudina annetta (Gray) (Lepidoptera, Nymphalidae, Charaxinae) I. Cabeça, apêndices e região cervical. Revista Brasileira de Zoologia, 21(2), 357-369.

Miller, J. S. (1987). Phylogenetic studies in the Papilioninae (Lepidoptera: Papilionidae). Bulletin of the American Museum of Natural History, 186(4), 365-512.

Miller, L. D. (1968). The higher classification, phylogeny and zoogeography of the Satyridae (Lepidoptera). Memoirs of the American Entomological Society, 24, 1-174.

Miller, L. D., & Brown, F. M. (1975). Notes on the rare Mexican pierid Prestonia clarki (Pieridae). Journal of the Lepidopterists' Society, 29(4), 256-258.

Moraes, S. S., & Duarte, M. (2009). Morfologia externa comparada das três espécies do complexo Telchin licus (Drury) (Lepidoptera, Castniidae) com uma sinonímia. Revista Brasileira de Entomologia, 53(2), 245-265.

Murillo-Ramos, L., Hernández-Mejía, C., & Llorente-Bousquets, J. (2016). The phylogenetic position of Aphrissa (Lepidoptera: Pieridae: Coliadinae) within its relatives the ancient American Catopsilias. Zootaxa, 4147(5), 538-550.

Paluch, M., Casagrande, M. M., & Mielke, O. H. H. (2008). Morfologia externa do adulto de Actinote melanisans (Nymphalidae: Heliconiinae: Acraeini). Revista Brasileira de Zoologia, 25(3), 456-478.

Reuter, E. R. (1896). Über die Palpen der Rhopalocera. Ein Beitrag zur Erkenntnis der verwandtschaftlichen Beziehungen unter den Tagfaltern. Acta Societatis Scientiarum fennicae, 22(1), 1-577.

Salazar, J. A. (2008). Some studies on palpi belonging to Neotropical charaxids and notes on the wing pattern and behavior of several genera (Lepidoptera: Nymphaloidea, Charaxidae). Boletín científico. Museo de Historia natural. Universidad de Caldas, 12, 171-205.

Schaus, W. (1920). New species of Lepidoptera in the United States National Museum. Proceedings of the United States national Museum, 57(2307), 107-152.

Sellier, R. (1977). Étude ultramorphologique des sensilles antennaires des Sésiidés. Essai sur l’origine et les étapes de la morphogenèse des sensilles antennaires des lépidoptères. Annales de la Société Entomologique de France (N. S.), 13(2), 347-367.

Shields, V. D. C. (2008). Ultrastructure of Insect Sensilla. In J. L. Capinera (Ed.), Encyclopedia of Entomology (2 Ed., pp. 4073-4087). Berlin: Springer-Verlag.

Song, Y. Q., Sun, H. Z., & Wu, J. X. (2016). Ultrastructural characteristics of the proboscis and the labial palp pit organ in the oriental fruit moth, Grapholita molesta. Bulletin of Insectology, 69(1), 59-66.

Sourakov, A., Duehl, A., & Sourakov, A. (2012). Foraging behavior of the blue Morpho and other tropical butterflies: The chemical and electrophysiological basis of olfactory preferences and the role of color. Psyche (Cambridge), 2012(378050), 1-10.

Stange, G. (1992). High resolution measurement of atmospheric carbon dioxide concentration changes by the labial palp organ of the moth Heliothis armigera (Lepidoptera: Noctuidae). Journal of Comparative Physiology A, 171, 317-324.

Stange, G., Monro, J., Stowe, S., & Osmond, C. B. (1995). The CO2 sense of the moth Cactoblastis cactorum and its probable role in the biological control of the CAM plant Opuntia stricta. Oecologia, 102, 341-352.

Stange, G., & Stowe, S. (1999). Carbon-Dioxide Sensing Structures in Terrestrial Arthropods. Microscopy Research and Technique, 47, 416-427.

Treat, A. E., & Niederman, L. (1967). Three species of Proctolaelaps (Acarina, Mesostigmata) from noctuid moths. American Museum Novitates, 2312, 1-12.

Vázquez, L. (1956). Reconsideración taxonómica de Prestonia clarki Schaus - Phoebis (Prestonia) clarki (Schaus) - Lepidoptera-Pieridae. Anales del Instituto de Biología, Universidad Nacional Autónoma de México, 26(2), 477-487.

Viloria, A. L. (2001). Sistemática práctica: recuento de morfología externa, reconocimiento y uso de las homologías en los Rhopalocera adultos (Insecta: Lepidoptera). Primer Diplomado de Mariposas Tropicales, Universidad del Valle, Cali, Colombia.

Vom Rath, O. (1888). Über die Hautsinnesorgane der Insekten. Z Wissensch Zool. 46, 413-454.

Zacharuk, R. Y. (1985). Antennae and sensilla. In G. A. Kerkut & L. I. Gilbert (Eds.), Comprehensive insect physiology. Biochemistry and pharmacology (Vol. 6, pp. 1-69). Oxford: Pergamon Press.

Zhao, X. C., Tang, Q. B., Berg, B. G., Liu, Y., Wang, Y. R., Yan, F. M., & Wang, G. R. (2013). Fine structure and primary sensory projections of sensilla located in the labial-palp pit organ of Helicoverpa armigera (Insecta). Cell and Tissue Research, 353, 399-408.



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