Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://revistas.ucr.ac.cr/index.php/rbt/oai
Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca
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Keywords

common bean; recalcitrant species; genetic transformation; A. tumefaciens; gusA activit; nptII
frijol común; especies recalcitrantes; transformación genética; A. tumefaciens; actividad gusA; nptII

How to Cite

Solís-Ramos, L.-Y., Ortiz-Pavón, J.-C., Andrade-Torres, A., Porras-Murillo, R., Brenes-Angulo, A., & Castaño de la Serna, E. (2019). Agrobacterium tumefaciens-mediated transformation of common bean (Phaseolus vulgaris) var. Brunca. Revista De Biología Tropical, 67(S2), S83-S94. https://doi.org/10.15517/rbt.v67i2SUPL.37208

Abstract

Common bean is a crop recalcitrant to in vitro regeneration and therefore it lacks an efficient transformation protocol that can be reproduced using A. tumefaciens. The main goal of this study was to establish a protocol for A. tumefaciens mediated transformation of Phaseolus vulgaris var. Brunca by marker genes (gusA and nptII) together with the gene for trehalose-6-phosphate synthase from Saccharomyces cerevisiae (TPS1) used in other species to increase tolerance to abiotic stress. The β-glucuronidase activity was detected in 45 % of the LBA4404 ElectroMAX® pCAMBIA1301 infected explants. Transformed explants regenerated new shoots after four to five months period in a kanamycin rich media. Surviving plants were evaluated by PCR and presented an 0.5 % efficiency of transformation. The established protocol for genetic transformation of common bean has two additional advantages with respect to previous reports: (1) it allows for obtaining transformed regenerants and (2) the genetic transformation was stable for the selective gene.

https://doi.org/10.15517/rbt.v67i2SUPL.37208
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