Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://revistas.ucr.ac.cr/index.php/rbt/oai
Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy
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Keywords

emerging economies
molecular testing
SARS-CoV-2
saliva
loop mediated isothermal amplification
economías emergentes
pruebas moleculares
SARS-CoV-2
saliva
amplificación isotérmica mediada por bucle

How to Cite

Segura-Ulate, I., Bolívar-González, A., Madrigal-Redondo, G., Nuñez-Corrales, S., & Gatica-Arias, A. (2022). Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy. Revista De Biología Tropical, 70(1), 173–189. https://doi.org/10.15517/rev.biol.trop.v70i1.47407

Abstract

Introduction: Most successful cases of COVID-19 pandemic mitigation and handling have relied on extensive reverse-transcription quantitative polymerase chain reaction (RT-qPCR). However, many emerging economies have struggled with current molecular testing demands due to economic, technical and technological constraints. Objective: To define a potential diagnostic protocol to increase testing capacity in current and post-pandemic conditions. Methods: We reviewed the literature, patents and commercial applications, for alternatives. Results: We found a good potential in saliva samples, viral inactivation and quick RNA extraction by heating; the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity. Conclusions: Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations.

https://doi.org/10.15517/rev.biol.trop..v70i1.47407
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