Keywords
hepatotoxinas
microcistinas
represa de Yacyretá.
Microcystis aeruginosa
hepatotoxins
microcystins
Yacyretá dam.
How to Cite
Abstract
Cyanobacteria constitute the main toxin producers in inland water ecosystems and have extensive global distribution. The presence of hepatotoxins in aquatic environments is hazardous to human and animal health; even though the presence and identification of hepatotoxic microcystins in rivers and reservoirs of the world have been confirmed by several studies in the last few years. Herein, we studied the abundance and toxicity of Microcystis aeruginosa in the Argentine section of the Paraná River at the beginning of the Middle Paraná (Corrientes Hydrometer), near Corrientes city (27º28´ S - 58º51´ W) and approximately 220 km downstream of the Yacyretá dam (High Paraná). The Paraná River basin, with a drainage area of 3.1 x 106 km2 and 3 965 km in length, is the second largest catchment of South America, after that of the Amazon. The Paraná River is the main source of drinking water supply for the Northeastern Argentine region. Phytoplankton samples were collected and environmental variables were measured in a monthly basis (exceptionally fortnightly), from March 2004 to June 2008. Fifty-eight samples were analyzed for phytoplankton density and biomass. Five samples were used for toxicity testing; the latter were obtained during the cyanobacteria blooms from 2005 to 2008. Phytoplankton counts were performed with an inverted microscope, and biomass was expressed as biovolume. Bioassays with mice and high-performance liquid chromatography (HPLC) analysis were performed to evaluate the presence of cyanotoxins. Phytoplankton mainly consisted of Cryptophyta, Chlorophyta and Bacillariophyta. Microcystis aeruginosa was identified during the warmer months each year (November to March). Density varied between 189 and 25 027 cells/mL (1-10 colonies/mL) and biomass from 0.34 to 44 mm3/L. Taking into account the number of cells, the highest abundance occurred in April 2004 (25 027 cells/mL), coinciding with the largest biovolume (44 mm3/L). All mice subjected to intraperitoneal injections with samples obtained during bloom episodes showed positive results for the presence of hepatotoxins. Three microcystins variants: LR, RR and [D-Leu1] Mcyst-LR were detected by analysis with semi-preparative high-performance liquid chromatography with diode array detector system (HPLC-PDA). This constitutes the first report of microcystins recorded during M. aeruginosa blooms in the Argentine stretch of the Paraná River at the beginning of the Middle Paraná (Corrientes Hydrometer), approximately 220 km downstream of the Yacyretá dam (High Paraná).
References
Andrinolo, D., Pereira, P., Giannuzzi, L., Aura, C., Massera, S., Caneo, M., Caixach, J., Barco, M., & Echenique, R. (2007). Ocurrence of Microcystis aeruginosa and Microcystins in the Río de La Plata river (Argentina). Acta Toxicológica Argentina, 15, 13-18.
Andrinolo, D., & Ruiz, M. (2011). Manejo y Control de cianobacterias en lagos, reservorios y ríos. Alertas. In L. Giannuzzi (Ed.), Cianobacterias como determinantes ambientales de la Salud (pp. 103-116). Buenos Aires: Ministerio de Salud de la Nación.
Azevedo, S. M. F. O., Carmichael, W. W., Jochimsen, E. M., Rinehart, K., Lau, S., Shaw, G., & Eaglesham, G. (2002). Human intoxication by microcystins during renal dialysis treatment in Caruaru-Brazil. Toxicology, 181/182, 441-446.
Bonetto, A. A., Zalocar de Domitrovic, Y., & Vallejos, E. R. (1982). Contribución al conocimiento del fitoplancton del Paraná Medio. I. Ecosur, 9, 189-212.
Bonetto, A. A., Wais, I., & Castello, H. P. (1989). The increasing damming of the Paraná basin and its effects on the lower reaches. Regulated Rivers: Research and Management, 4, 333-346.
Calijuri, M. Do C., Alves, M. S. A., & Alves Dos Santos, A. C. (2006) Cianobacterias e Cianotoxinas em Águas Continentais. Sao Carlos: RiMa.
Cazenave, J., Bistoni, M., Pesce, S. F., & Wunderlin, D. A. (2005). Differencial detoxification and antioxidant response in diverse organs of Coridoras paleatus experimentally exposed to microcystin- RR. Aquatic Toxicology, 75, 178-190.
Chorus, I., & Bartram, J. (Eds.) (1999). Toxic Cyanobacteria in Water. A guide to public health consequences, monitoring and management. London: World Health Organization, E & FN Spon.
Culling, C. F. A. (1975). Handbook of Histological Techniques. London: Butterworths.
De León, L., & Yunes, J. (2001). First report of a microcystin-containing bloom of the cyanobacterium Microcystis aeruginosa 2001 in the Río de la Plata river, South America. Environmental Toxicology, 16, 110-112.
Domitrovic, H. A., Bechara, J. A., Jacobo, R., Flores Quintana, C. I., & Roux, J. P. (1994). Mortandad de peces en el río Paraná provocada por sobresaturación de gases: causas y lesiones. Revista de Ictiología, 23, 49-54.
Forastier, M. E. (2012). Diversidad y toxicidad de Cyanophyta (Cyanobacteria) del nordeste argentino (Tesis inédita de doctorado). Universidad Nacional del Nordeste, Corrientes-Argentina.
Garrido, G. G. (1999). Composición y abundancia del zooplancton en dos estaciones de muestreo del embalse de Yacyretá, Argentina en las primeras etapas después del llenado a cota 76 m s.n.m. Revista de Ictiología, 7, 27-35.
Hillebrand, H., Dürselen, C. D., Kirschtel, D. Pollingher, U., & Zohary, T. (1999). Biovolume calculation for pelagic and benthic microalgae. Journal of Phycology, 35, 403-421.
Komárek, J. (2006). Review. Cyanobacterial taxonomy: Current problems and Prospects for the integration of traditional and Molecular Approaches. Algae, 21, 349-375.
Lund, J. W. G., Kipling, C., & Le Cren, E. D. (1958). The inverted microscope method of estimating algal numbers and the statistical basis of estimations by counting. Hydrobiologia, 11, 143-170.
Meichtry de Zaburlín, N. (1999). La comunidad fitoplanctónica durante las primeras etapas de llenado del embalse de Yacyretá, Argentina. Revista de Ictiología, 7, 15-26.
Meichtry de Zaburlín, N. (2002). Estructura de la comunidad fitoplanctónica en el embalse Yacyretá (Argentina-Paraguay) (Tesis de Maestría). Universidad Nacional del Litoral, Santa Fe.
Meichtry de Zaburlín, N. R., Llano, V., & Martens, I. S. (2011). Cyanobacteria del Embalse Yacyretá en relación a los cambios de cota. Boletín de la Sociedad Argentina de Botánica, 46(Supl.), 18.
National Research Council (1985). Guide for the Use and Care of Laboratory Animals. Bethesda, MD: National Institute of Health.
Neiff, J. J., Poi de Neiff, A. S. G., Patiño, C. A. E., & Basterra de Chiozzi, I. (2000). Prediction of colonization by macrophytes in the Yacyretá reservoir of the Paraná river (Argentina and Paraguay). Revista Brasileira de Biologia, 60, 615-626.
Orfeo, O., & Stevaux, J. (2002). Hydraulic and morphological characteristics of middle and upper reaches of the Paraná River (Argentina and Brazil). Geomorphology, 44, 309-322.
Paerl, H. W., & Huisman, J. (2008). Blooms like it hot. Science, 320, 57-58.
Park, H. D., Iwami, C., Watanabe, M. F., Harada, K. I., Okino, T., & Hayashi, H. (1998). Temporal variabilities of the concentration of intra- and extracellular microcystin and toxic Microcystis species in a hypertrophic lake, lake Suwa, Japan (1991-1994). Environmental Toxicology and Water Quality, 13, 61-72.
Peso, J., & Bechara, J. A. (1999). Estructura del zoobentos del embalse de Yacyretá, Argentina en dos estaciones de muestreo, antes y después del llenado a cota 76 m.s.n.m. Revista de Ictiología, 7, 37-47.
Pizzolón, L., Tracanna, B., Prósperi, C., & Guerrero, R. (1999). Cyanobacterial blooms in Argentinean inland waters. Lakes & reservoirs: Research and Management, 4, 1-5.
Rosso, L., Caixach, J., Kolman, M., Sedan, D., Flores, C., Echenique, R. O., Salerno, G., Giannuzzi, L., & Andrinolo, D. (2012). Caracterización filogenética y toxicológica de una cepa de microcystis aeruginosa, productora de Microcystina aislada del ambiente. 7mo Congreso de Medio Ambiente. AUGM. UNLP.
Sournia, A. (Ed.) (1978). Phytoplankton Manual. Paris: UNESCO (United Nations Educational, Scientific and Cultural Organization).
Utermöhl, H. (1958). Zur Vervollkommnung der quantitativen Phytoplankton-Methodik. Mitteilung Internationale Vereinigung Limnologie, 9, 1-38.
Vollenweider, R. A. (Ed.) (1974). A manual on methods for measuring primary production in aquatic environments. Oxford: Blackwell scientific publications (IBP Handbook n. 12).
Welker, M., Sigrid, H., & Steinberg, C. (1999). Hepatotoxic cyanobacteria in the shallow lake Müggelsee. Hydrobiologia, 408/409, 263-268.
Younes, O., Zaccaro, M. C., Zulpa, G., Storni, M., Stella, A. M., Bossio, J. C., Tanuz M., & Del Campo, F. (2011). A single microcystin in a toxic Microcystis bloom from the Río de la Plata river (Argentina). International Journal of Environmental Analytical Chemistry, 91, 525-536.
Zalocar de Domitrovic, Y., & Maidana, N. I. (1997). Taxonomic and ecological studies of the Paraná River diatom flora (Argentina). Bibliotheca Diatomologica, 34, 1-122.
Zalocar de Domitrovic, Y., & Vallejos, E. R. (1982). Fitoplancton del río Alto Paraná. Variación estacional y distribución en relación a factores ambientales. Ecosur, 9, 1-28.
Zalocar de Domitrovic, Y., & Forastier, M. E. (2007). Historia de las floraciones del río Paraná en el tramo argentino. In J. Feyen, L. F. Aguirre, & R. Mónica Moraes (Eds.). Congreso Internacional sobre Desarrollo, Medio Ambiente y Recursos Naturales: Sostenibilidad a múltiples niveles y escalas (pp. 1291-1298). Cochabamba: Publicación de la Universidad Mayor de San Simón.
Zalocar de Domitrovic, Y., & Forastier, M. E. (2008). Las cianobacterias del nordeste argentino: caracteres generales e importancia ecológica. In S. L. Casco, I. Basterra, & J. J. Neiff (Eds.), Manual de Biodiversidad de Chaco, Corrientes y Formosa (pp. 57-77). Corrientes: EUDENE.
Zalocar de Domitrovic, Y., Devercelli, M., & García de Emiliani, M. O. (2007a). Phytoplankton. In M. H. Iriondo, J. C. Paggi, & M. J. Parma (Eds.), The Middle Paraná River: Limnology of a Subtropical Wetland (pp. 177-203). Berlin Heidelberg: Springer-Verlag.
Zalocar de Domitrovic, Y., Poi de Neiff, A. S. G., & Casco, S. L. (2007b). Abundance and diversity of phytoplankton in the Paraná River (Argentina) 220 km downstream of the Yacyretá reservoir. Brazilian Journal of Biology, 67, 53-63.
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