Agronomía Costarricense ISSN Impreso: 0377-9424 ISSN electrónico: 2215-2202

OAI: https://revistas.ucr.ac.cr/index.php/agrocost/oai
Populations and identification of fungi causing postharvest molds, on pineapple peduncles in two regions in Costa Rica
PDF (Español (España))

Keywords

ananas comosus
Penicillium
molds
postharvest phases
waxes
ananas comosus
Penicillium
mohos
fases poscosecha
ceras

How to Cite

Castro Chinchilla, J., & Umaña Rojas, G. (2015). Populations and identification of fungi causing postharvest molds, on pineapple peduncles in two regions in Costa Rica. Agronomía Costarricense, 39(3). https://doi.org/10.15517/rac.v39i3.21792

Abstract

Pineapple peduncle mold is an important postharvest problem in Costa Rica and it causes fruit rejection. The objective of this study was to identify and quantify the most important fungi in different postharvest phases. Monthly samplings were performed during one production year in 2 regions of Costa Rica. The main genera of fungi were identified and characterized at the molecular level. The colony forming units (CFU) were determined in disinfection water, wax, cooling rooms air and in the peel and peduncle of fruits before (NP) and after (P) processing with the common postharvest treatments of the farms. Fruits were stored in cooling rooms during 22 days and at the end incidence and severity of peduncle molds were evaluated. During the year, changes in fungi populations were observed in all postharvest phases and in the fruits, with higher populations in wax than in disinfection water. Fungi population and molds were higher in the peduncle of NP fruits as compared with P fruits, coincident with larger mold populations at the end of storage. Fungi recovered in the cooling rooms air could also be a source for peduncle molds development. Penicillium purpureogenum, P. diversum and Penicllium sp., were the main fungi identified, with an in vitro high sporulation rate and growing in the peduncle. Moreover, different commercial practices, such as waxing and cooling, where spores were captured, can enhance the peduncle molds development, so it is considered important the cleaning of cooling rooms, as well as developing mechanisms to avoid accumulation in wax of important populations of microorganisms.
https://doi.org/10.15517/rac.v39i3.21792
PDF (Español (España))

References

ALVAREZ A.M., NISHIJIMA W.T. 1987. Postharvest diseases of papaya. Plant Disease 71(8):681-686.

AMIRI A., BOMPEIX G. 2005. Diversity and population dynamics of Penicillium spp., on apples in pre-and postharvest environments: consequences for decay development. Plant Pathology 54:74-81.

ANTHONY S., ABEYWICKRAMA K., DAYANANDA R., WILSON S., ARAMBEWELA L. 2004. Fungal pathogens associated with banana fruit in Sri Lanka and their treatment with essential oils. Mycopathologia 157:91-97.

ARAUZ F. 1994. Elementos básicos de patologia poscosecha de frutas y hortalizas. I Taller regional de manejo poscosecha de productos de interés para el Trópico. San José, Costa Rica. pp. 1-10.

ARAYA B., CASCANTE M. 2000. Manejo post-cosecha de productos agrícolas. San José, Costa Rica. Editorial UNED. 219 p.

BAERT K., DEVLIEGHERE F., BO L., DEBEVERE J., DE MEULENAER B. 2008. The effect of inoculum size on the growth of Penicillium expansum in apples. Food Microbiology 25:212-217.

BARNETT H.L., HUNTER B.B. 1972. Illustrated genera of imperfect fungi. Third edition. Minnesota, USA. Burguess Publishing Company. 241 p.

BARQUERO A. 2010. Estudio comparativo de la eficacia de cinco desinfectantes y optimización del mejor de ellos para la etapa de desinfección de piña fresca en la empresa Banacol. Práctica Dirigida Escuela de Tecnología de Alimentos para optar por el grado de licenciatura en Tecnología de Alimentos. Costa Rica, Universidad de Costa Rica. 57 p.

BARTH M., HANKINSON T., ZHUANG H., BREIDT F. 2009. Microbiological spoilage of fruits and vegetables. In: W. Sperber and M. Doyle (eds.). Compendium of the microbiological spoilage of foods and beverages. New York, USA. Springer. 367 p.

BARTHOLOMEW D.P., PAULL R.E., ROHRBACH K.G. 2003. The pineapple, botany, production and uses. New York, USA. CABI Publishing. 301 p.

BOCCO A., CUVELIER M., RICHARD H., BERSET C. 1998. Antioxidant activity and phenolic composition of citrus peel and seed extracts. Journal of Agriculture and Food Chemistry 46:2123-2129.

BUTTNER M.P., STETZENBACH L.D. 1993. Monitoring airborne fungal spores in an experimental indoor environment to evaluate sampling methods and the effects of human activity on air sampling. Applied and Environmental Microbiology 59(1):219-226.

CAB. 2002. Phoma herbarum. In: Descriptions of fungi and bacteria. CABI. 151: Sheet 1501. Consultado el 25 de noviembre de 2013. Disponible en www.cabi.org/dfb

CANAPEP 2015. Estadísticas de exportaciones Costa Rica. Consultado el 25 de febrero de 2015. Disponible en www.canapep.com CASTRO J. 2015. Hongos causantes de mohos en frutos de piña poscosecha, su incidencia y relación con las condiciones climáticas en dos zonas de Costa Rica. Tesis de maestría, Universidad de Costa Rica. Costa Rica. 111 p.

CHANDA S., BARAVALIA Y., KANERIA M., RAKHOLIYA K. 2010. Fruit and vegetable peelsstrong natural source of antimicrobics, pp. 444-450. In: M. Vilas, (ed.). Current Research Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Badajoz, España.

DENNIS C. 1983. Post-harvest pathology of fruits and vegetables. London, England. Academic Press. 264 p.

ELLIS M.B. 1976. More dematiaceous hyphomycetes. Surrey, England. CAB. 507 p.

FRENCH E., HEBERT T. 1980. Métodos de investigación fitopatológica. San José, Costa Rica. IICA. 289 p.

GARCÍA A., RODRÍGUEZ M. 2011. Manual de buenas prácticas agrícolas para la producción de piña en Costa Rica. San José, Costa Rica. Banacol-RepCar. 66 p.

GUO C., YANG J., WEI J., LI Y., XU J., JIANG Y. 2003. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutrition Research 23:1719-1726.

HU H., LI X., DONG C., CHEN W. 2011. Effects of wax treatment on quality and postharvest physiology of pineapple fruit in cold storage. African Journal of Biotechnology 10(39):7592-7603.

HUI Y., BARTA J., CANO M., GUSEK T. 2006. Handbook of fruits and fruit processing: science and technology. Iowa, USA. Blackwell Publishing. 697 p. Consultado el 30 de setiembre de 2011. Disponible en http:// books.google.com

JACOBS A., VAN WYK P.S., MARASAS W.F., WINGFIELD B.D., WINGFIELD M.J., COUTINHO T.A. 2010. Fusarium ananatum sp. Nov in the Giberella fujikuroi species complex from pineapples with fruit rot in South Africa. Fungal Biology 114:515-527.

LARA I., BELGE B., GOULAO L.F. 2014. The fruit cuticle as a modulator of postharvest quality. Postharvest Biology and Technology 87:103-112.

LATA B., TRAMPCZYNSKA A., PACZESNA J. 2009. Cultivar variation in apple peel and whole fruit phenolic composition. Scientia Horticulturae 121:176-181.

LÓPEZ C. 2012. Frecuencia de los principales organismos asociados con el moho en el corte de piña (Ananas comosus) Var. Dorada Extradulce, patogenicidad y sensibilidad al fungicida triadimefon. Tesis licenciatura en Agronomía. Costa Rica. Universidad de Costa Rica. 71 p.

MAG. 2010. Manual de buenas prácticas agrícolas para la producción de piña. Heredia, Costa Rica. MAG, SFE. 133 p.

MALINS A. 1991. Second regional workshop in tropical fruit crops, papaya, pineapple and mango. Antigua & Barbuda. IICA. 97 p.

MITRA S. 1997. Postharvest physiology and storage of tropical and subtropical fruits. London, England. Cab International. 423 p.

MOKBEL M.S., HASHINAGA F. 2005. Antibacterial and antioxidant activities of banana peel (Musa AAA cv. Cavendish) fruits peel. American Journal of Biochemistry and Biotechnology 1(3):125-131.

MONTERO M., ROJAS M.A., MARTÍN O. 2008. Effect of packaging conditions on quality and shelf-life of fresh-cut pineapple (Ananas comosus). Postharvest Biology and Technology 50:182-189.

MORALES H., MARÍN S., RAMOS A., SANCHIS V. 2010. Influence of post-harvest technologies applied during cold storage of apples in Penicillium expansum growth and patulin accumulation: A review. Food control 21:953-962.

MORALES H., SANCHIS V., COROMINES J., RAMOS A., MARÍN S. 2008. Inoculum size and intraspecific interactions affects Penicillium expansum growth and patulin accumulation in apples. Food Microbiology 25:378-385.

MOURICHON X. 1998. Pineapple fruit core rot (black spot) and leathery pocket: review and prospects. Acta Horticulturae, Proceedings of 2nd International Pineapple Symposium. Montpellier, Francia. 508 p.

PAULL E., DUARTE O. 2011. Tropical fruits. London, England. Cab International. 2nd Edition. 400 p.

PAULL R.E., REYES M.E. 1996. Preharvest weather conditions and pineapple fruit translucency. Scientia Horticulturae 66:59-67.

PITT J., HOCKING A.D. 2009. Fungi and food spoilage. New York, USA. Springer. 519 p. Consultado el 10 de marzo de 2015. Disponible en books.google. co.cr/books PROCOMER. 2015. Portal estadístico de comercio exterior. Consultado el 25 de febrero de 2015. Disponible en www.procomer.com

PRUSKY D., GULLINO M. 2010. Postharvest Pathology. New York, USA. Springer. 1150 p.

REYES D. 2012. Compuestos GRAS para el control de patógenos poscosecha in vitro en mango (Mangifera indica L.), piña (Ananas comosus L.) y papaya (Carica papaya L.), y pruebas de eficacia in vivo en piña. Tesis de licenciatura, Universidad de Costa Rica, Costa Rica. 92 p.

REYES M. 1999. Use of microbial antagonists to control postharvest black rot on pineapple fruit. Tesis de doctorado, Universidad de Hawai, Hawai.128 p.

SEIFERT K., MORGAN G., GAMS W., KENDRICK B. 2011. The genera of hyphomycetes. Utrecht, Netherlands. CBS-KNAW Fungal Biodiversity Centre. 997 p.

SNOWDON A. 2000. A color Atlas of post-harvest diseases & disorders of fruits and vegetables. Vol 1. Florida, USA. CRC Press. 302 p.

STĘPIEŃ L., KOCZYK G., WAŚKIEWICZ A. 2011a. FUM cluster divergence in fumonisins-producing Fusarium species. Fungal Biology 115:112-123.

STĘPIEŃ L., KOCZYK G., WAŚKIEWICZ A. 2011b. Genetic and phenotypic variation of Fusarium proliferatum isolates from different host species. Journal of Applied Genetics 52:487-496.

STĘPIEŃ L., KOCZYK G., WAŚKIEWICZ A. 2013. Diversity of Fusarium species and mycotoxins contaminating pineapple. Journal of Applied Genetics 54(3):367-380.

TORTORA G., FUNKE B., CASE C. 2007. Introducción a la microbiología. Buenos Aires, Argentina. Editorial Médica Panamericana. 9° Edición. 963 p.

VON ARX J.A. 1974. The genera of fungi. Sporulating in pure culture. Leutershausen, Germany. J. Cramer. 315 p.

WATANABE T. 1994. Soil and seed fungi. Morphologies of cultured fungi and key to species. Tokyo, Japan. Lewis Publishers. 411 p.

WILLS R., McGLASSON B., GRAHAM D., JOYCE D. 1998. Postharvest. An introduction to the physiology & handling of fruit, vegetables & ornamentals. Sydney, Australia. Cab International.1 ed. 262 p.

Comments

Downloads

Download data is not yet available.