Natural outcrossing in common bean in Costa Rica.
DOI:
https://doi.org/10.15517/am.v25i1.14490Keywords:
Phaseolus vulgaris, floral biology, gene flow, natural hybrids, morphological marker, pollen, pollinators.Abstract
The objective of the research was to evaluate the natural outcrossing rate between two varieties of common bean in Alajuela and Fraijanes, localities in the Central Valley of Costa Rica. The experimental plot included three blocks with ten 15.0 m long rows, 0.8 m The black-seeded varieties UCR 55 and Guaymí were growing in Fraijanes and Alajuela in the central block respectively; while the white-seeded variety PAN 68 was planted in the two lateral blocks. The experiments were carried out from 2002 thru 2003 in Fraijanes and from 2002 thru 2007 in Alajuela. The variables: wind direction, seasonal effect and distance from the pollen source were evaluated. There was a synchrony in the flowering time of the varieties in both localities. All plants of the white-seeded variety were numbered and harvested in the five nearest rows to the black-seeded varieties. Natural outcrossing was recorded as a percentage of seedlings with purple hypocotyl in the F1 progeny of PAN 68 (variety that shows green hypocotyls). In Alajuela the rate of natural outcrossing varied from 0,000 to 0.096%, while in Fraijanes it did from 0.004 to 0.033%. The general allogamy was of 0.031%, a lower outcrossing if compared to trials carried out in other countries. The natural outcrossing was not affected by the dry or rainy season nor the wind direction, but it was inversely affected by the distance from the pollen source in Alajuela (r2=0.9622, p≤0.05); It was nil from 4 m away from the pollen source.Downloads
References
Ahrné, K., J. Bengtsson, y T. Elmqvist. 2009. Bumble
bees (Bombus spp) along a gradient of increasing
urbanization. PLoS ONE 4(5):e5574. doi:10.1371/
journal.pone.0005574.
Alan, J., y C. Moh. 1966. Determinación del porcentaje de
cruzamiento natural en el frijol común (Phaseolus
vulgaris L.) en Alajuela, Costa Rica. Turrialba
(2):156-158.
Aragão, F., G. Vianna, M. Albino, y E. Rech. 2002. Transgenic
dry bean tolerant to the herbicide glufosinate
ammonium. Crop Sci. 42:1298-1302. Aragão, F., y J. Faria. 2009. First transgenic geminivirus resistant plant in the field. Nat. Biotechnol. 27:1086-1088.
Araya, R., y J. Acosta. 2000. Adaptación del frijol a suelos de baja fertilidad bajo presión de enfermedades foliares en Costa Rica. Agron. Mesoam. 11(2):31-36.
Bassett, M. 1996. List of genes – Phaseolus vulgaris L. Annual Report of the Bean Improvement Coopertive (USA) 39:1-19.
Bliss, F. 1980. Common bean. En: W. Ferh, y H. Hadley, editores, Hybridization of crops plants. American Society of Agronomy and Crop Science, Wisconsin, USA. p. 273-284.
Bonfim, K., J. Faria, E. Nogueira, É. Mendes, y F. Aragão. 2007. RNAi-mediated resistance to Bean golden mosaic virus in genetically engineered common bean (Phaseolus vulgaris). Mol. Plant-Microbe Interact. 20:717-726.
Broughton, W., G. Hernández, M. Blair, S. Beebe, P. Gepts, y J. Vanderleyden. 2003. Beans (Phaseolus spp.) - model food legumes. Plant Soil 252(1):55-128.
Brunner, B., y J. Beaver. 1989. Estimation of outcrossing of the common bean in Puerto Rico. HortScience 24:669-771.
Chaves, N., R. Araya, y D. Debouck. 2009. Flujo de genes entre frijol común y silvestre en Costa Rica. Agron. Mesoam. 20:237-244.
Corbet, S., I. Williams, y J. Osborne. 1991. Bees and the pollination of crops and wild flowers in the European community. Bee World 72:47-59.
Crispín, A. 1960. Cruzamiento natural en frijol. Agricultura Técnica en México 11:38-39.
Ferreira, J., M. Álvarez, M. Fueyo, A. Roca, y R. Giraldez. 2000. Determination of the outcrossing rates of Phaseolus vulgaris L. using seed protein markers. Euphytica 113:259-263.
Ferreira, J., J. De Souza, A. Teixeira, F. De Lanes, P. Cecon, y A. Borém. 2007. Gene flow in common bean (Phaseolus vulgaris L.). Euphytica 153:165-170.
Free, J. 1966. The pollination of beans Phaseolus multiflorus and Phaseolus vulgaris by honeybees. J. Apicult. Res. 5(2):87-91.
Gepts, P., A. González, R. Papa, J. Acosta, A. Wong, A. Delgado. 2000. Outcrossing in Mexican wild and domesticated populations of common bean. Annual Report of the Bean Improvement Cooperative 43:25-26.
Gepts, P., y R. Papa. 2003. Possible effects of (trans)gene flow from crops on the genetic diversity from landraces and wild relatives. Environ. Biosafety Res. 2(2):89-103.
Gutiérrez, M., D. Soto, y M. Alpízar. 1997. Cuarenta años de observaciones meteorológicas en la Estación Experimental Fabio Baudrit Moreno. Boletín Técnico Estación Experimental Fabio Baudrit Moreno (BOLTEC) 30(2):1-14.
Gutiérrez, M.V., D. Soto, y M. Alpízar. 1999-2002. El clima en la Sub-Estación Experimental Fraijanes, en un ecosistema montano bajo y húmedo. Revista de Agricultura Tropical (Costa Rica) 32:39-45.
Hails, R. 2000. Genetically modified plants: the debate continues. Trends Ecol. Evol. 15:14-18.
Hails, R. 2002. Assessing the risks associated with new agricultural practices. Nature 418:685-688.
Harlan, J., y J. De Wet. 1971. Toward a rational classification of cultivated plants. Taxon 20:509-517.
Holdridge, L.R. 1982. Ecología basada en zonas de vida. Instituto Interamericano de Cooperación para la Agricultura (IICA), San José, Costa Rica.
Ibarra, F., N. Ellstrand, y J. Giles. 1996. Multiple paternity in common bean (Phaseolus vulgaris L., Fabaceae). Am. J. Bot. 83(6):749-758.
Ibarra, F., B. Ehdaie, y J. Waines. 1997. Estimation of outcrossing rate in common bean. Crop Sci. 37:60-65.
Ibarra, F., D. Barnhart, B. Ehdaie, K. Knio, y J. Waines. 1999. Effects of insect tripping on seed yield of common bean. Crop Sci. 39:428-433.
Kwapata, K., T. Nguyen, y M. Sticklen. 2012. Genetic transformation of common bean (Phaseolus vulgaris L.) with the Gus color marker, the Bar herbicide resistance, and the barley (Hordeum vulgare) HVA1 drought tolerance genes. Int. J. Agron. Volume 2012, Article ID 198960.doi:10.1155/2012/198960.
León, J. 1987. Botánica de los cultivos tropicales. 2 ed. Instituto Interamericano de Cooperación para la Agricultura (IICA), San José, Costa Rica.
Mackie, W., y F. Smith. 1935. Evidence of field hybridization in beans. J. Am. Soc. Agron. 27:903-909.
Miranda, S. 1971. Cruzamiento natural en frijol. Agricultura Técnica en México 3(2):48-52.
ONS (Oficina Nacional de Semillas de Costa Rica). 2012. Reglamento técnico para la producción de semilla certificada de frijol. http://www.ofinase.go.cr. (Consultado 7 abr. 2013).
Ortega, S. 1974. Polinización cruzada natural de la caraota (P. vulgaris L.) en Venezuela. Agronomía Tropical 24(1):27-32.
Papa, R., y P. Gepts. 2003. Asymmetry of gene flow and differential geographical structure of molecular diversity in wild and domesticated common bean (Phaseolus vulgaris L.) from Mesoamerica. Theor. Appl. Genet. 106:239-250.
Pinchinat, A. 1967. Cruzamiento natural en el frijol común (Phaseolus vulgaris L.) en Costa Rica. En: Editorial Nuestra América, editor, VII Reunión Latinoamericana de Fitotecnia. Maracay, Venezuela. p. 208-209.
Ramírez, P., A. Reyes, J. Estrada, F. Castillo, S. Cruz, y L. Barrios. 2000. Cruzamiento natural de frijol común en los valles altos de México. Revista Fitotecnia Mexicana 23(2):277-292.
Royer, M., M. Gonçalves, C. Scapim, P. Soares, V. Filho, y Y. Terada. 2002. Outcrossing in common bean. Crop Breed. Appl. Biotechnol. 2(1):49-54.
Stoetzer, H. 1984. Natural cross-polinization in bean in Ethiopia. Annual Report of the Bean Improvement Cooperative 27:99-100.
Triana, B., M. Iwagana, H. Rubiano, y M. Andrade. 1993. A study of allogamy in wild Phaseolus vulgaris. Annual Report of the Bean Improvement Cooperative 36:20-21.
Tucker, C., y J. Harding. 1975. Outcrossing in common bean Phaseolus vulgaris L. J. Am. Soc. Hortic. Sci. 100:283-285.
Vieira, C. 1960. Sobre a hibridação natural em Phaseolus vulgaris L. Ceres 11:103-107.
Webster, B., C. Tucker, y S. Lynch. 1977. A morphological study of the development of reproductive structures of Phaseolus vulgaris L. J. Am. Soc. Hortic. Sci. 102:640-643.
Webster, B., R. Ross, y T. Evans. 1982. Nectar and the nectary of Phaseolus vulgaris L. J. Am. Soc. Hortic. Sci. 107:497-503.
Weinstein, A. 1926. Cytological studies on Phaseolus vulgaris. Am. J. Bot. 13(4):248-263.
Wells, W., W. Isom, y J. Waines. 1988. Outcrossing rates of six common bean lines. Crop Sci. 28:177-178.
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