Differential histopathology of three bacterial diseases that affect the hepatopancreas of penaeid shrimp.

Authors

  • Alexander Varela-Mejías Laboratorio de Patologías y Parasitología de Crustáceos. Nicoya, Guanacaste, Costa Rica.
  • Nelson Peña-Navarro Universidad Técnica Nacional

DOI:

https://doi.org/10.15517/am.v27i1.21887

Keywords:

shrimp farming, bacterial infections, differential diagnosis.

Abstract

The objective of this work was to characterize the injuries caused by the acute hepatopancreatic necrosis; the necrotizing hepatopancreatitis, and septic hepatopancreatic necrosis in shrimp farming. The information collected from many literature sources associated with the observations made in tissue samples from infected shrimp was analyzed. Histopathology was used to process the affected tissues, analyzing tropism of injuries, the special and differential characteristics between these diseases. Among the main differences mentioned: the acute hepatopancreatic necrosis injuries move forward from the proximal areas of the tubules of the hepatopancreas to the distal regions and acute cell causing massive and intensive sloughing. In peels necrotising hepatopancreatitis tubular necrotic cells present with basophilic cytoplasm and granular appearance leading to granulom formation atrophy in the terminal phase; and finally the septic hepatopancreatic necrosis injury presents a randomly advance of injuries with pyknotic epithelial cell detachment, forming hemocytic and immediate inflammatory response. Distinguish between these diseases provides the possibility that, by histopathological analysis can make a differential diagnosis between them.

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References

Aguirre-Guzmán, G., F. Ascencio, and D. Saulnier. 2005. Pathogenicity of Vibrio penaeicida for white shrimp Litopenaeus vannamei: a cysteine protease-like exotoxin as a virulence factor. Dis. Aquat. Org. 67: 201-207.

Bell, T. A., and D.V. Lightner, 1988. A handbook of normal penaeid shrimp histology. World Aquaculture Society, Baton Rouge, LA, USA.

Bradley-Dunlop, B.J., C. Pantoja, and D.V. Lightner. 2004. Development of monoclonal antibodies for detection of necrotizing hepatopancreatitis in penaeid shrimp. Dis. Aquat. Org. 60:233-240.

Crabtree, B.G., M.M. Erdman, D.L. Harris, and I. Turney. 2006. Preservation of necrotizing hepatopancreatitis bacterium (NHPB) by freezing tissue collected from experimentally infected Litopenaeus vannamei. Dis. Aquat. Org. 70:175-179.

Cuéllar-Anjel, J. 2008. Métodos de diagnósticos de enfermedades en camarones marinos de cultivo. En: V. Morales, y J. Cuéllar- Anjel, editores, Guía técnica: patología e inmunología de camarones penaeidos.

Programa CYTED Red II-D Vannamei, PAN. p. 1-54.

Cuéllar-Angel, J., D.V. Lightner., y C. Pantoja. 2012. Síndrome de mortalidad temprana o síndrome de necrosis hepatopancreática aguda. Panorama Acuícola Magazine 17:42-43.

Han, J.E., L. Mohney, K. Tang, C. Pantoja, and D.V. Lightner. 2015a. Plasmid mediated tetracycline resistance of Vibrio parahaemolyticus associated with acute hepatopancreatic necrosis disease (AHPND) in shrimps. Aquaculture Reports 2:17-21.

Han, J.E., K. Tang, L. Tran, and D. Lightner. 2015b. Photorhabdus insect-related (Pir) toxin-like genes in a plasmid of Vibrio parahaemolyticus, the causative agent of acute hepatopancreatic necrosis disease (AHPND) of shrimp. Dis. Aquat. Org. 113:33-40.

Joshi, J., J. Srisala, V.H. Truong, I.T. Chen, B. Nuangsaeng, O. Suthienkul, C.F. Lo, T.W. Flegel, K. Sritunyalucksana, and S. Thitamadee. 2014. Variation in Vibrio parahaemolyticus isolates from a single Thai shrimp farm experiencing an outbreak of acute hepatopancreatic necrosis disease (AHPND). Aquaculture (428–429):297-302.

Lightner, D.V. 1996. A handbook of shrimp pathology and diagnostic procedures for diseases of cultured penaeid shrimp. World Aquaculture Society, Baton Rouge, LA, USA.

Morales-Covarrubias, M.S. 2008. Enfermedades bacteriales. En: V. Morales, y J. Cuéllar- Anjel, editores, Guía técnica: patología e inmunología de camarones peneidos. Programa CYTED Red II-D Vannamei, PAN. p. 117-134.

Morales-Covarrubias, M.S. 2010. Enfermedades del camarón: detección mediante análisis en fresco e histopatología. Editorial Trillas. México, D.F., MEX.

Morales-Covarrubias, M.S. 2013. Camarón análisis en fresco, herramienta de diagnóstico. 1era. edición. CIAD-OIRSA, MEX.

Morales-Covarrubias, M.S., A.G. Osuna, A. García, D.V. Lightner, and J.C. Mota. 2006. Prevalence of necrotizing in female broodstock of white shrimp Penaeus vannamei with unilatral eyestalk ablation and hormone injection. J. Aquatic Anim. Health 18:19-25.

Morales-Covarrubias, M.S, y B. Gómez-Gil. 2014. Enfermedades bacterianas de camarones. En: V. Morales, y J. Cuéllar-Anjel, editores, Guía técnica: patología e inmunología de camarones penaeidos, 2da ed. OIRSA, Rep. de Panamá, PAN. p. 167-171.

Nunan, L., C. Pantoja, S. Gómez-Jiménez, and D.V. Lightner. 2013. “Candidatus Hepatobacter penaei’’ an shrimp Penaeus vannamei in the Hepatopancreas of the marine intracellular pathogenic enteric bacterium (Crustacea: Decapoda). Appl. Environ. Microbiol. 79(4):1407.

Nunan, L.M., C. Pantoja, and D.V. Lightner. 2008. Improvement of a PCR method for the detection of necrotizing hepatopancreatitis in shrimp. Dis. Aquat. Org. 80:69-73.

OIE (Organización Mundial de Sanidad Animal). 2012. Manual de diagnóstico en animales acuáticos. 6 ed., OIE, Paris, FRA.

Pantoja, C. 2014. Otras enfermedades bacterianas que afectan el hepatopáncreas del camarón peneido (EMSdiagnóstico diferencial). Presentación durante el 3er Curso teórico práctico de actualización en patología e inmunología del camarón blanco Litopenaeus vannamei. OIRSA, PAN.

Pantoja, C., y D.V. Lightner. 2014. EMS/AHPND: Descripción de la enfermedad en Asia y América. En: Morales, V. y J. Cuéllar-Anjel, editores, Guía técnica: patología e inmunología de camarones penaeidos. OIRSA, Rep. de Panamá, PAN. p.172-177.

Peña, N., R. Vargas, y A. Varela. 2013. Productos naturales como estimuladores del sistema inmunológico de Litopenaeus vannamei, infectado con Vibrio parahaemolyticus. Agron. Mesoam. 24:133-147.

Tran, L., L. Nunan, R. Redman, L. Mohney, C. Pantoja, K. Fitzsimmons, and D.V. Lightner. 2013. Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp. Dis. Aquat. Org. 105:45-55.

Varela, A. 2007. Manual para la interpretación de resultados de laboratorio. Alicorp, Publicis- Asociados, PER.

Varela, A., y N. Peña. 2014a. Fluctuaciones térmicas y su relación con el virus del síndrome de las manchas en Litopenaeus vannamei cultivado en el Golfo de Nicoya¨ Rev. UTN Informa 68:80-83.

Varela, A., y N. Peña. 2014b. Síndrome de la mortalidad temprana (EMS/AHPNS) en camarones cultivados: Una revisión. Repertorio Científico 17:25-30.

Varela, A., y N. Peña. 2015. Hepatopancreatitis Necrotizante asociada al Fenómeno del Niño, en cultivos de camarones del Golfo de Nicoya. Repertorio Científico 18:29-34.

Vincent, A., Breland, V.M, and J. Lotz. 2004. Experimental infection of Pacific white shrimp Litopenaeus vannamei with Necrotizing Hepatopancreatitis (NHP) bacterium by per os exposure. Dis. Aquat. Org. 61:227-233.

Vincent, A., and J. Lotz. 2005. Time course of necrotizing hepatopancreatitis (NHP) in experimentally infected Litopenaeus vannamei and quantification of NHPbacterium using real-time PCR. Dis. Aquat. Org. 67:163-169.

Vincent, A., and J. Lotz. 2007. Effect of salinity on transmission of necrotizing hepatopancreatitis bacterium (NHPB) to Kona stock Litopenaeus vannamei. Dis. Aquat. Org. 75:265-268.

Published

2015-12-09

How to Cite

Varela-Mejías, A., & Peña-Navarro, N. (2015). Differential histopathology of three bacterial diseases that affect the hepatopancreas of penaeid shrimp. Agronomía Mesoamericana, 27(1), 73–80. https://doi.org/10.15517/am.v27i1.21887