Common bean genotype interaction - Rhizobium strain.

Authors

  • Luis A. Gómez Estación Experimental "La Renée". Ap.6. La Habana, Cuba.
  • Germán Hernández Estación Experimental "La Renée". Ap.6. La Habana, Cuba.
  • Tamara Sánchez Estación Experimental "La Renée". Ap.6. La Habana, Cuba.
  • Vidalina Toscano Estación Experimental "La Renée". Ap.6. La Habana, Cuba.
  • Mercedes Sánchez Estación Experimental "La Renée". Ap.6. La Habana, Cuba.

DOI:

https://doi.org/10.15517/am.v9i1.24648

Abstract

The capacity of five common bean genotypes from the germ plasm bank at CIAT, Colombia (Río Tibaji, APN 18, Carioca, G 14665, and G19441) to fix nitrogen from the atmosphere was evaluated. An experiment was carried out with plants grown in 16-liter capacity plastic boxes with a highly aerated and nitrogen-free nutrient solution (400 ml of air/solution liter/min). In the first box the CIAT 899 Rhizobium strain was innoculated, and in another box the CR 477 strain from Costa Rica, both from CIAT; a third box was reserved for CF1 of national origino The experimental design consisted of random blocks with four repetitions. In order to determine the dry mass of the aereal part (DMAP), the radicular dry mass (RDM), and the nodular dry mass (NDM), the plants were cut in the R7 developmental phase. With respect to NDM, the carioca genotype and the CF1 strains were statistically superior to the rest (P<0,00l); however, the major nitrogen fixers were the G 14665 genotype - the best combination between NDM and fixation efficiency - and the CIAT 899 strain, which was 2.4 times more efficient (N mgl NDM g) than the rest. This shows that the NDM production is not a determining variable when genotypes or strains with a high capacity for nitrogen fixation in the atmosphere are being selected.

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References

ABIDOO, R. c.; GEORGE l; BOHLOOL B. B.; SINGLETON P. W. 1990. Influence of elevation and applied nitrogen on rhizosphere colonization and competition for nodule occupancy by different rhizobial strain on fieldgrown soybean and common bean. Canadian Joumal of Microbiology. 36: 92-96.

BEURNARD, P.; MICHELLON, R. 1986. Effect de l' inoculation der haricot par deux souche de Rhizobium phaseoli. Agronomie Tropicale 41: 128-131.

BLISS, EA. 1993. Breeding common bean for improved biological nitrogen fixation. Plant and Soil 152:71-79.

CASTRO, L.; URIBE L.; ALVARADO A, 1993. Efecto del enriquecimiento del inoculante de Rhizobium con dosis crecientes de P sobre el crecimiento del frijol común (Phaseolus vulgaris L.). Agronomía Costarricense 17: 55-59.

DOBEREIGNER, J. 1988. Biotechnology: Recent advances in biological nitrogen fixation. VI Japan-Brazil Symposium on Science and Technology. Editado por Academia de Ciencias de Estado de Sao Paulo (4): 145-160.

DREVON, J.J.; KALIA U.P.; HECKMANN M.O.; PEDELAHORE P. 1988. In situ open - flow assay of acetylene and oxigen. Plant Physiology and Biochemistry 26:73- 78.

DREVON,].J.1994. Resumen de los resultados en frijol común del Programa de Cooperación Colombia - Cuba - Francia. Mimeografiado. 84 p.

GÓMEZ, L.A.; VADEZ V.; HERNÁNDEZ G.; SÁNCHEZ T.; TOSCANO V. 1996. Eficiencia de uso al fósforo en diez genotipos de frijol común (Phaseolus vulgaris L.). Agronomía Mesoamericana 7:41-46

GRACEDA-CABRERA, O.A.; J.J. PEÑA-CABRIOLA. 1995. Use of different indicators for measuring fixation in common bean (Phaseolus vulgaris L.) under field conditions in Nuclear Technique in Soil - Plant Study for Sustainable Agriculture and Environmental Preservation (Proceeding of a Symposium, Vienna 17-21 October) pp 712-714. Printed IAEA in Austria. 735 p.

GRAHAM, P. 1988. The contribution of biological nitrogen fixation to plant production. An overview of the symposium and its implications. Plant and Soil 108: 1-6.

HAROARSON, G. 1993. Methods for enhancing symbiotic nitrogen fixation. Plant and Soil 152: 1-17.

HAROARSON, G.; OANSON S.K.A. 1993. Methods for measuring biological nitrogen fixation in grain legumes. Plant and Soil 152:19-23.

HANSON, R.A. 1993. Measurements of N2 fixation by common bean in central Brazil as affected by different reference crops. Plant and Soil 152:53-58.

MARTINEZ, E.; FLORES M.; BROM S.; ROMERO D.; DAVILA G.; PALACIOS R 1988. Rhizobium phaseoli: A molecular genetics view. Plant and Soil 108: 179-184.

MOXLEY, J.c.; HUME O.J.; SMITH O.L. 1986. N2 fixation and competitiveness of Rhizobium phaseoli strains isolated from Ontario soils. Canadian Journal of Plant Science 66: 825-836.

RIBET, J.; OREVON, J.J . 1995. Increase in permeability to oxygen and in oxygen uptake of soybean nodules under limiting phosphorus nutrition. Physiology Plantarum. 94:1-7.

PACOVSKY, RS.; BAYRE H.G.; G.1. BETHLENFALVAY. 1984. Symbiotic interactions between strain of Rhizobium phaseoli and cultivates of Phaseolus vulgaris L. Crop Science 24:101-105.

TRUCHET, M. 1992. A Joint genetic and cytological, approach of the development biology of the R. Meliloti - M. Sativa symbiosis. 6to Curso Internacional sobre fijación del nitrógeno. INRA, Montpellier, Francia. Mimeografia. Tomo 1.

VAOEZ, V.; J.J. DREVON y O. BECK. 1996. Pattern of growth N accumulation and P use efficiency related 10 N nutrition and genotype variability in common bean (en prensa).

Published

2016-05-31

How to Cite

Gómez, L. A., Hernández, G., Sánchez, T., Toscano, V., & Sánchez, M. (2016). Common bean genotype interaction - Rhizobium strain. Agronomía Mesoamericana, 9(1), 93–97. https://doi.org/10.15517/am.v9i1.24648

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