Morphological/pathogenic variability of Fusarium verticillioides in La Ciénega/Chapala, Mexico and inoculation techniques evaluation
DOI:
https://doi.org/10.15517/am.v34i1.49679Keywords:
Zea Mays, pudrición de mazorca, técnica de inoculaciónAbstract
Introduction. Corn ear rot caused by Fusarium verticillioides (F. verticillioides) is one of the most important diseases in the world. In Mexico it causes losses of up to 30 %. The increase in the levels of this disease in the last years requires inspections in producing areas to identify the main strains that damage the grain. Objectives. To determine the morphological and pathogenic variability of F. verticillioides isolates from corn samples from the corn producing area of La Ciénega/Chapala, Mexico, and to identify the best technique for inoculating the pathogen in ears. Materials and Methods. Twenty isolates of F. verticillioides were obtained from samples of corn kernels with disease symptoms. The isolates were used to inoculate, with the use of different inoculation techniques, developing corn cobs (R2) of P-3057 cultivated in Tepatitlán, Jalisco during the Spring/Summer 2019 agricultural cycle under rainfed conditions. The incidence and severity of the disease (SDI) of the isolates and the microscopic morphology of the strains were evaluated. Results. F. verticillioides isolates differed in colony color, mycelial type, abundance, and vegetative mycelial coloration. The incidence and severity were statistically different between F. verticillioides isolates and inoculation techniques tested. Interaction between isolates and inoculation technique was observed. The disease incidence showed values of 82 and 95% with aspersion and toothpick inoculation techniques, respectably. The SDI varied between 8 and 15.5 % with the aspersion and toothpick techniques, in that order. Conclusions. F. verticillioides strains showed variability for pathogenicity and severity of infection. Pathogenicity was related to the inoculation technique used. The toothpick inoculation technique was the one that presented the highest incidence and severity of infection.
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References
Blacutt, A. A., Gold, S. E., Voss, K. A., Gao, M., & Glenn, A. E. (2018). Fusarium verticillioides: Advancements in understanding the toxicity, virulence, and niche adaptations of a model mycotoxigenic pathogen of maize. Phytopathology, 108(3), 312–326. https://doi.org/10.1094/PHYTO-06-17-0203-RVW
Brown, W. D., Butchko, R. A. E., Busman, M., & Proctor, R. H. (2012). Identification of gene clusters associated with fusaric acid, fusarin and perithecial pigment production in Fusarium verticillioides. Fungal Genetics and Biology, 49(7), 521–532. https://doi.org/10.1016/j.fgb.2012.05.010
Covarelli, L., Stifano, S., Beccari, G., Raggi, L., Lattanzio, V. M. T., & Albertini, E. (2012). Characterization of Fusarium verticillioides strains isolated from maize in Italy: Fumonisin production, pathogenicity and genetic variability. Food Microbiology, 31(1), 17–24. https://doi.org/10.1016/j.fm.2012.02.002
de la Torre-Hernández, M. E., Sánchez-Rangel, D., Galeana-Sánchez, E., & Plasencia-de la Parra, J. (2014). Fumonisinas–Síntesis y función en la interacción Fusarium verticillioides-maíz. TIP Revista Especializada en Ciencias Químico-Biológicas, 17(1), 77–91. https://doi.org/10.1016/S1405-888X(14)70321-3
Duncan, K. E., & Howard, R. J. (2010). Biology of maize kernel infection by Fusarium verticillioides. Molecular Plant-Microbe Interactions, 23(1), 6–16. https://doi.org/10.1094/MPMI-23-1-0006
Fideicomisos Instituidos en Relación con la Agricultura. (2016). Panorama agroalimentario maíz. Recuperado en noviembre 2021 de https://bit.ly/3gBQGUB
García-Lara, S., & Bergvinson, D. (2007). Programa integral para reducir pérdidas postcosecha en maíz. Agricultura Técnica en México, 33(2), 181–189.
Ghimire, K., Petrović, K., Kontz, B. J., Bradley, C. A., Chilvers, M. I., Mueller, D. S, Smith, D. L., Wise, K. A., & Mathew, F. M. (2019). Inoculation method impacts symptom development associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on soybean (Glycine max). Plant Disease, 103(4), 677–684. https://doi.org/10.1094/PDIS-06-18-1078-RE
Gitonga, Z. M., De Groote, H., Kassie, M., & Tefera, T. (2013). Impact of metal silos on households’ maize storage, storage losses and food security: An application of a propensity score matching. Food Policy, 43, 44–55. https://doi.org/10.1016/j.foodpol.2013.08.005
Gómez, K. A., & Gómez, A. A. (1984). Statistical procedures for agricultural research (2nd Ed.). John Wiley & Sons, Inc.
Gordon, M. R. (2021). El maíz en Panamá: Características, requerimientos y recomendaciones para su producción en ambientes con alta variabilidad climática. Instituto de Investigación Agropecuaria de Panamá.
Lanubile, A., Maschietto V., Borrelli V. M., Stagnati L., Logrieco A. F., & Marocco, A. (2017). Molecular basis of resistance to Fusarium ear rot in Maize. Frontiers in Plant Science, 8, Article 1774. https://doi.org/10.3389/fpls.2017.01774
Leslie, J. F., & Summerell, B. A. (2006). The Fusarium laboratory manual. Blackwell Publishing Professional. https://onlinelibrary.wiley.com/doi/pdf/10.1002/9780470278376.fmatter
López-Castillo, L. M., Silva-Fernández, S. E., Winkler, R., Bergvinson, D. J., Arnason, J. T., & García-Lara, S. (2018). Postharvest insect resistance in maize. Journal of Stored Products Research, 77, 66–76. https://doi.org/10.1016/j.jspr.2018.03.004
Mesterházy, Á., Lemmens, M., & Reid, L. M. (2012). Breeding for resistance to ear rots caused by Fusarium spp. in maize – A review. Plant Breeding, 131(1), 1–19. https://doi.org/10.1111/j.1439-0523.2011.01936.x
Missmer, S. A., Suarez, L., Felkner, M., Wang, E., Merrill Jr., A. H., Rothman, K. J., & Hendricks, K. A. (2006). Exposure to fumonisins and the occurrence of neural tube defects along the Texas-Mexico border. Environmental Health Perspectives, 114(2), 237–241. https://doi.org/10.1289/ehp.8221
Morales, L., Zila, C. T., Moreta Mejía, D. E., Montoya Arbelaez, M., Balint-Kurti, P. J., Holland, J. B., & Nelson, R. J. (2019). Diverse components of resistance to Fusarium verticillioides infection and fumonisin contamination in four maize recombinant inbred families. Toxins, 11(2), Article 86. https://doi.org/10.3390/toxins11020086
Naz, R., Bano, A., Nosheen, A., Yasmin, H., Keyani, R., Shah, S. M., Anwar, Z., & Roberts, T. H. (2021). Induction of defense-related enzymes and enhanced disease resistance in maize against Fusarium verticillioides by seed treatment with Jacaranda mimosifolia formulations. Scientific Reports, 11, Article 59. https://doi.org/10.1038/s41598-020-79306-x
Poulsen, H.M., Ridao, A. del C., & Castaño, F. (2013). Comparación entre técnicas de inoculación de Fusarium verticillioides en espigas de maíz. RIA. Revista de Investigaciones Agropecuarias, 39(3), 312–317.
Rangel-Castillo, A., Valadez-Moctezuma, E., & Lozoya Saldaña H. (2017). Caracterización molecular y patogénesis de Fusarium asociado al amarillamiento del trigo. Revista Fitotecnia Mexicana, 40(4), 439–450. https://doi.org/10.35196/rfm.2017.4.439-450
Rauwane, M. E., Ogugua, U. V., Kalu, C. M., Ledwaba, L. K., Adugna, A. W., & Ntushelo, K. (2020). Pathogenicity and virulence factors of Fusarium graminearum including factors discovering using next generation sequencing technologies and proteomics. Microorganisms, 8(2), Article 305. https://doi.org/10.3390/microorganisms8020305
Secretaría de Economía. (1995). NMX-FF-034. Productos alimenticios no industrializados. Cereales. Maíz (Zea mays l.) Especificaciones y métodos de prueba. Gobierno de México.
Shabeer, S., Tahira, R., & Jamal, A. (2021). Fusarium spp. mycotoxin production, diseases, and their management: An overview. Pakistan Journal of Agricultural Research, 34(2), 278–293. https://doi.org/10.17582/journal.pjar/2021/34.2.278.293
Silva, J. J., Viano, H. P., Ferranti, L. S., Oliveira, A. L. M., Ferreira, J. M., Ruas, C. F., Ono, E. Y. S., & Fungaro, M. H. P. (2017). Genetic structure of Fusarium verticillioides and occurrence of fumonisins in maize grown in Southern Brazil. Crop Protection, 99, 161–167. https://doi.org/10.1016/j.cropro.2017.05.020
Sistemas de Información Agroalimentaria y Pesquera. (2021). Datos abiertos. Anuario estadístico de la producción agrícola. Estadística de producción agrícola de 2020. http://infosiap.siap.gob.mx/gobmx/datosAbiertos.php
Solano-Báez, A. R., De León-García De Alba, C., Valdovinos-Ponce, G., Silva-Rojas, H. V., & Soto-Rojas, L. (2011). La pigmentación de Fusarium verticillioides (Sacc.) como factor de virulencia en plántulas de maíz. Agronomía Mesoamericana, 22(2), 297–307. https://doi.org/10.15517/AM.V22I2.11803
Thompson, M. E. H., & Raizada, M. N. (2018). Fungal pathogens of maize gaining free passage along the silk road. Pathogens, 7(4), Article 81. https://doi.org/10.3390/pathogens7040081
Tom, A., & Patel, S. (2021). Dynamic association of Fusarium verticillioides with maize and its biological control. The Pharma Innovation Journal, 10(7), 1010–1019. https://www.thepharmajournal.com/archives/?year=2021&vol=10&issue=7&ArticleId=6999
Torres-Sánchez, L., & López-Carrillo, L. (2010). Consumo de fumonisinas y daños a la salud humana. Salud Pública de México, 52(5), 461–467. https://saludpublica.mx/index.php/spm/article/view/6999
Vázquez Badillo, M. E., & Moreno Martínez, E. (2016). Postcosecha de granos. Almacenamiento en México. Claridades Agropecuarias, 271, 3–6. https://www.gob.mx/cms/uploads/attachment/file/185452/revista_271.pdf
Wang, W., Wang, B., Sun, X., Qi, X., Zhao, C., Chang, X., Ibrahim Khaskheli, M., & Gong, G. (2021). Symptoms and pathogens diversity of corn Fusarium sheath rot in Sichuan Province, China. Scientific Report, 11, Article 2835. https://doi.org/10.1038/s41598-021-82463-2
Wit, M., Warzecha, R., Mirzwa-Mróz, E., Jablońska, E., Piotr, O., Waskiewicz, A., & Wakuliński, W. (2011). Susceptibility of flint and dent maize ears to Fusarium species. Phytopathologia, 60, 35–45.
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Copyright (c) 2022 Norma Y. Zácamo-Velázquez, Javier Ireta-Moreno, Yolanda Salinas-Moreno, Víctor M. Gómez-Rodríguez, Humberto Ramírez-Vega, Raquel Martínez-Loperena
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