Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

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Effect of the probiotic <i>Bacillus subtilis</i> on the growth and food utilization of tilapia (<i>Oreochromis niloticus</i>) and prawn (<i>Macrobrachium rosenbergii</i>) under laboratory conditions
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Keywords

probiotics
bacillus subtilis
aquaculture
laboratory conditions
oreochromis niloticus
macrobrachium ronsenbergii
probioticos
bacillus subtilis
acuacultura
laboratorio
oreochromis niloticus
macrobrachium ronsenbergii

How to Cite

Günther, J., & Jiménez-Montealegre, R. (2004). Effect of the probiotic <i>Bacillus subtilis</i> on the growth and food utilization of tilapia (<i>Oreochromis niloticus</i>) and prawn (<i>Macrobrachium rosenbergii</i>) under laboratory conditions. Revista De Biología Tropical, 52(4), 937–943. Retrieved from https://revistas.ucr.ac.cr/index.php/rbt/article/view/15588

Abstract

Three experiments were conducted to analyze the effect of the probiotic Bacillus subtilis on the growth of juvenile tilapia (Oreochromis niloticus) and freshwater prawn (Macrobrachium rosenbergii). The experiments were conducted under laboratory conditions, minimizing the indirect effects of the probiotic on the water quality and leaving only the possible bactericidal and digestion-support effects. A model of stress was also designed in tilapia to compare the effect with tilapia under normal conditions. The dose in the food was 0.1 % of the probiotic (5x108 CFU/g and 99.9 % maltrine) in the dry diet. Every 14 days the animals were weighed in group (tilapias ± 0.1 g, prawns ± 0.001 g) to estimate average body weight. In the first experiment (tilapia) the specific growth rate (SGR) and the feed conversion ratio (FCR) were bad in relation with the factor probiotic, but the differences were not significant. In the second experiment (tilapia) both the SGR and the FCR deteriorated with the addition of B. subtilis to the diet; the difference was significant to 94%. The stress factor, on the contrary, caused a notable worsening of both the growth and the food utilization. In the experiment with prawns the addition of B. subtilis caused a light deterioration of the growth and of the food utilization, with a statistical probability of mistake of 10% in case of the growth. During the experiment the direct effects over the digestive system should have prevailed, either by the contribution of macro- and micronutrients, or by the enzymes that contribute to the digestion. The negative effect due to the addition of the probiotic to the food was small (about 10% in both the SGR and the FCR) being difficult to detect statistically. The reports on the positive action of probiotics on the growth in aquatic animals have been conducted mainly in ponds, and our information does not contradict directly a possible positive action of B. subtilis in this type of systems. Since the effect on the digestive system seems to be relatively small, in those environments the effect might be compensated by other positive effects on water quality, and by bactericidal effects on pathogenic bacteria.
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References

Ashraf, A. 2000. Probiotics in fish farming. Evaluation of a candidate bacterial mixture. Tesis de Licenciatura,

SLU, Suecia.

Bogut, I., Z. Milakovic, Z. Bukvic, S. Brkic & R. Zimmer. 1998. Influence of probiotic (Streptococcus faecium M74) on growth and content of intestinal microflora in carp (Cyprinus carpio). J. Animal Sci. 43: 231-235.

Briones-Fourzán, P. & E. Lozano-Álvarez. 2003. Factors affecting growth of the spiny lobsters Panulirus gracilis

and Panulirus inflatus (Decapoda: Palinuridae) in Guerrero, México. Rev. Biol. Trop. 51: 165-174.

Campaña Torres, A., H. Villarreal Colmenares, R. Civera Cerecedo & L.R. Martínez Córdova. 2003. Efecto

del nivel proteico de la dieta sobre el desarrollo de juveniles de la langosta australiana Cherax quadricarinatus

(Decapoda: Parastacidae). Rev. Biol. Trop. Vol. 51: 749-752.

EPA (US. Environmental Protection Agency). 2000. Biopesticide registration action document: Bacillus subtilis. 10 p.

Gatesoupe, F.J., T. Arakawa & T. Watanabe. 1989. The effect of bacterial additives on the production rate and

dietary value of rotifers as food for Japanese flounder, Paralichthys olivaceus. Aquaculture 83: 39-44.

Gatesoupe, F.J. 1991. The effect of three strains of lactic bacteria on the production rate of rotifers, Brachionus

plicatilis, and their dietary value for larval turbot, Scophthalmus maximus. Aquaculture 96: 335-342.

Gatesoupe, F.J. 1999. The use of probiotics in aquaculture. Aquaculture 180: 147-165.

Geiger, F. 2001. Untersuchungen ueber die wirkung oral verabreichter β -glukane und probiotika bei zierfischen

gegenueber infektionen durch fakultativ pathogene bakterien. Tesis de Doctorado, Escuela Veterinaria Hannover. 115 p.

Gildberg, A., A. Johansen & J. Bogwald. 1995. Growth and survival of Atlantic salmon (Salmo salar) fry given

diets supplemented with fish protein hydrolysate and lactic acid bacteria during a challenge trial with Aeromonas salmonicida. Aquaculture 138: 23-34.

Gómez-Márquez, J.L. 1998. Age and growth of Oreochromis niloticus (Perciformes: Cichlidae) in Mexico. Rev. Biol. Trop. 46: 929-936.

Gómez-Márquez, J.L., B. Peña-Mendoza, I.H. Salgado- Ugarte & Guzmán-Arroyo. 2003. Reproductive aspects of Oreochromis niloticus (Perciformes: Cichlidae) at Coatetelco lake, Morelos, Mexico. Rev. Biol. Trop. 51: 221-228.

Hooper, L.V. & J.I. Gordon. 2001. Commensal host-bacterial relationships in the gut. Science 292: 1115-1118.

Iwama, G.K. & A.F. Tautz. 1981. A simple growth model for salmonids in hatcheries. Canadian J. Fish and Aquatic Sci. 38: 649-656.

Jory, D.E. 1998. Use of probiotics in penaeid shrimp growout. Aquac. Mag. 24: 62-67.

Kim, S.M. & W.J. Kim. 2002. Purification and characteristics of Bacillus subtilis JM-3 salt- and acid-tolerant protease derived from anchovy sauce. Annual Meeting and Food Expo, Anaheim, California. 2 p.

Noh, S.H., K. Han, T.H. Won & Y.J. Choi. 1994. Effect of antibiotics, enzyme, yeast culture and probiotics on growth performance of Israeli carp. Korean J. Animal Sci. 36: 480-486.

Queiroz, J. & C. Boyd. 1998. Effect of a bacterial innoculum in channel catfish ponds. J. World Aquaculture Soc. 29: 67-73.

Re-Araujo, A.D. & M. de J. Acosta Ruiz. 2003. Ensayo de diferentes lecitinas en la dieta de juveniles de Penaeus vannamei (Crustacea: Decapoda). Rev. Biol. Trop. 51: 743-748.

Rengpipat, P., W. Phianphak, S. Piyatiratitivorakul & P. Menasveta. 1998. Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture 167: 301-313.

Verschuere L., G. Rombaut, P. Sorgeloos & W. Verstraete. 2000. Probiotic bacteria as biological control agentsin aquaculture. Mol. Micr. Biol. Rev. 64: 651-671.

Wang, Y.G., K.L. Lee, M. Najiah, M. Shariff & M.D. Hassan. 2000. A new bacterial white spot syndrome (BWSS) in cultured tiger shrimp Penaeus monodon and its comparison with white spot syndrome (WSS) caused by virus. Dis. Aquatic Organisms 41: 9-18.

REFERENCIAS DE INTERNET

Hui-Rong, Li, Yu Yong, Ji Wei-Shang & Xu Huai-Shu. 1999. The Effect of Alken Clear-Flo 1200 used in

grow-out ponds of Penaeus japonicus. Alken Murray Corporation. (Consultado: February, 26, 2002, http://

www.alken-murray.com/China99.htm).

Rothschild, P. 1993. The Bacillus subtilis story. (Consultado: May, 6, 2002, http://www.absolutehealth.org/crit2.

html).

Wang, Xiang-Hong, Li Jun, Ji Wei-Shang & Xu Huai-Shu. 1998. Application of probiotics in Aquaculture. Alken

Murray Corporation. (Consultado: February, 26, 2002, http://www.alken-murray.com/China98.htm).

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