Supplementary Files
Keywords
envelope protein
genotype
phylogenetic analysis
the central highlands
virus del dengue
proteína tipo cubierta
genotipo
análisis filogenético
Altiplano Central.
How to Cite
Abstract
Dengue fever is perhaps the most important viral re-emergent disease especially in tropical and sub-tropical countries, affecting about 50 million people around the world every year. In the Central Highlands regions of Vietnam, dengue fever still remains as a major public health issue. Although four viral serotypes have been currently identified, dengue virus type 2 (DENV-2) was involved in the most important outbreaks during 2010-2012, especially, 2010 when the fatality rate highly increased. Detection of genotype of DENV-2 provided information on origin, distribution and genotype of the virus. In this study, DEN-2 isolated from dengue patients during the 2010-2012 epidemics was amplified and sequenced with E gene. The consensus sequences were aligned with reference E gene sequences of globally available Genbank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree. A total of 15 isolates (seven from 2010; one from 2011 and seven from 2012) were obtained from human serum samples. Phylogenetic analysis revealed that Asian genotype 1 is currently circulating locally in Central Highlands region. Isolates of this genotype were closely related to viruses from Thailand, Laos, and Cambodia. It indicated that these epidemics maybe imported into the Central Highlands region from South-East Asia neighbor countries. The study results would help in planning for prevention and control of dengue virus in Vietnam. Continuous monitoring of DENV genotypes is necessary to confirm the current findings and detect possible genotype shifts within the dengue viruses in the future.
References
Dat, D. T., & Huong, V. T. (2010). Epidemiologic characteristics of Dengue fever in the Central Highlands of Vietnam, 1998-2010. Journal of Tay Nguyen Preventive Medicine, 2, 1-3.
Duoc, P. T., Dat, D. T., Trang, L. T. T., & Van, N. T. H. (2014). Epidemiologic characteristics of Dengue fever in the Central Highlands of Vietnam, 2008-2012. Journal of Tay Nguyen Preventive Medicine, 1, 41-47.
Fahri, S., Yohan, B., Trimarsanto, H., Sayono, S., Hadisaputro, S., Dharmana, E., ... Sasmono, R. T. (2013). Molecular surveillance of dengue in Semarang, Indonesia revealed the circulation of an old genotype of dengue virus serotype-1. PLOS Neglected Tropical Diseases, 7(8), e2354. doi: 10.1371/journal.pntd.0002354.
Ferguson, N. M., Donnelly, C. A., & Anderson, R. M. (1999). Transmission dynamics and epidemiology of dengue: insights from age-stratified sero-prevalence surveys. Philosophical Transactions of the Royal Society B: Biological Sciences, 354(1384), 757-768. doi: 10.1098/rstb.1999.0428.
Henchal, E. A., & Putnak, J. R. (1990). The dengue viruses. Clinical Microbiology Reviews, 3(4), 376-396. doi: 10.12691/ajmr-3-3-4.
Holmes, E. C., & Burch, S. S. (2000). The causes and consequences of genetic variation in dengue virus. Trends in Microbiology, 8(2), 74-77. doi: 10.1016/S0966-842X(99)01669-8.
Huang, J. H., Su, C. L., Yang, C. F., Liao, T. L., Hsu, T. C., Chang, S. F., ..., & Shu, P. Y. (2012). Molecular characterization and phylogenetic analysis of dengue viruses imported into Taiwan during 2008-2010. The American Journal of Tropical Medicine and Hygiene, 87(2), 349-358. doi: 10.4269/ajtmh.2012.11-0666.
Kochel, T. J., Watts, D. M., Halstead, S. B., Hayes, C. G., Espinoza, A., Felices, V., ..., & Russell, K. L. (2002). Effect of dengue-1 antibodies on American dengue-2 viral infection and dengue haemorrhagic fever. Lancet, 360(9329), 310-312. doi: 10.1016/s0140-6736(02)09522-3.
Lambrechts, L., Fansiri, T., Pongsiri, A., Thaisomboonsuk, B., Klungthong, C., Richardson, J. H., ..., & Scott, T. W. (2012). Dengue-1 virus clade replacement in Thailand associated with enhanced mosquito transmission. Journal of Virology, 86(3), 1853-1861. doi: 10.1128/jvi.06458-11.
Lanciotti, R. S., Calisher, C. H., Gubler, D. J., Chang, G. J., & Vorndam, A. V. (1992). Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase-polymerase chain reaction. Journal of Clinical Microbiology, 30(3), 545-551. doi: 0095-1137/92/030545-07$02.00/0.
Lanciotti, R. S., Lewis, J. G., Gubler, D. J., & Trent, D. W. (1994). Molecular evolution and epidemiology of dengue-3 viruses. Journal of General Virology, 75(Pt 1), 65-75. doi: 10.1099/0022-1317-75-1-65.
Larkin, M. A., Blackshields, G., Brown, N. P., Chenna, R., McGettigan, P. A., McWilliam, H., ..., & Higgins, D. G. (2007). Clustal W and Clustal X version 2.0. Bioinformatics, 23(21), 2947-2948. doi: 10.1093/bioinformatics/btm404.
Mandl, C. W., Guirakhoo, F., Holzmann, H., Heinz, F. X., & Kunz, C. (1989). Antigenic structure of the flavivirus envelope protein E at the molecular level, using tick-borne encephalitis virus as a model. Journal of Virology, 63(2), 564-571. doi:10.1016/S0092-8674(02)00660-8.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10), 2731-2739. doi: 10.1093/molbev/msr121.
Twiddy, S. S., Farrar, J. J., Vinh Chau, N., Wills, B., Gould, E. A., Gritsun, T., ..., & Holmes, E. C. (2002). Phylogenetic relationships and differential selection pressures among genotypes of dengue-2 virus. Virology, 298(1), 63-72. doi: 10.1006/viro.2002.1447.
Vu, T. T., Holmes, E. C., Duong, V., Nguyen, T. Q., Tran, T. H., Quail, M., ..., & Simmons, C. P. (2010). Emergence of the Asian 1 genotype of dengue virus serotype 2 in Vietnam: in vivo fitness advantage and lineage replacement in South-East Asia. PLOS Neglected Tropical Diseases, 4(7), e757. doi: 10.1371/journal.pntd.0000757.
Wang, C., Katzelnick, L. C., Montoya, M., Hue, K. D., Simmons, C. P., & Harris, E. (2016). Evolutionarily Successful Asian 1 Dengue Virus 2 Lineages Contain One Substitution in Envelope That Increases Sensitivity to Polyclonal Antibody Neutralization. The Journal of Infectious Diseases, 213(6), 975-984. doi: 10.1093/infdis/jiv536.
WHO. (2012). Dengue: a global threat - global answers. Global strategy for dengue prevention and control 2012-2020. Geneva: WHO, 43p. ISBN: 9789241504034.
Wittke, V., Robb, T. E., Thu, H. M., Nisalak, A., Nimmannitya, S., Kalayanrooj, S., ... & Aaskov, J. G. (2002). Extinction and Rapid Emergence of Strains of Dengue 3 Virus during an Interepidemic Period. Virology, 301(1), 148-156. doi:10.1006/viro.2002.1549.
Zhang, C., Mammen, M. P., Jr., Chinnawirotpisan, P., Klungthong, C., Rodpradit, P., Monkongdee, P., ..., & Holmes, E. C. (2005). Clade replacements in dengue virus serotypes 1 and 3 are associated with changing serotype prevalence. Journal of Virology, 79(24), 15123-15130. doi: 10.1128/jvi.79.24.15123-15130.2005.
Comments
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2017 Revista de Biología Tropical