How to Cite
Abstract
Two laboratory maintenance systems of Trypanosoma rangeli were compared. The maintenance by weekly subinoculations in Tobie's culture medium and the intrafemoral inoculation of Rhodnius prolixus with cultured flagellates, resulted in loss of infectivity of the metacyclic salivarian trypomastigotes for mice, ten months after maintenance in culture. With the system of cyclical passes through culture-Rhodnius-mouse- culture-Rhodnius, the infectivity of the metacyclic trypomastigotes for mice, was maintained during the three years of the experiment.
The number and porcentage of metaeyclic trypomastigotes formed in the salivary glands of R. prolixus, previously inoculated intrafemorally or intracoelomically with culture forms of T. rangeli, did not show correlation with the inoculated dose, however the inoculated quantity demostrated a direct relation with the mortality rate of the insects.
The results indicate that T. rangeli requires an adequate maintenance system, so that under experimental condition the biological characteristics, normally expressed under natural conditions, are conserved.
References
Coutinho, J.O., & V. Nussenzweig. 1952. Lnfecçao experimental de triatomineos pelo Trypanosoma rangeli Tejera, 1920. Fol. Clin. Biol. (S. Paulo) 18: 181-188.
Cuba, C. A. 1975. Estudo de una cepa peruana de Trypanosoma rangeli IV Observações sobre la evoIução e morfogênesena hemocele e nas glándulas salivares de Rhodnius ecuadoriensis. Rev. lnst. Med. Trop. Silo Paulo 17: 283-297.
D' Alessandro, A. 1972. New experimental vectors of Colombian Trypanosoma rangeli. J. Med. Ent. 9: 187-195.
D' Alessandro, A. 1976. Biology of Trypanosoma (Herpetosoma) rangeli Tejera, 1920. In Biology of the Kinetoplastida. v. 1, p. 327-493, eds. W. H. R. Lumsden, D. A. Evans, London, New York and San Francisco, Academic Press.
Guhl, F. & C. J. Marinkelle. 1982. Antibodies against Try panosoma cruzi in mice infected with T. rangeli. Ann. Trop. Med. Parasitol. 76: 361.
Miles, M. A., J. R. Arias, S.A.S. Valente, R. D. Naiff, A.A. de Sousa, M.M. Puda, J.A.N. Lima & R. A. Cedillos. 1983. Vertebrate hosts and vectors of Trypanosoma rangeli in the Amazon basin of Brasil. Am. J. Trop. Med. Hyg. 32: 1251-1259.
Schottelius, J., 1982. Differentiation between Trypanosoma cruzi and T. rangeli by their different complement sensitivity. Tropenmed. Parasit. 33: 147-150.
Schottelius, J. & V. Müller. 1984. Interspecific differentiation of Trypanosoma cruzi, Trypanosoma conorhini and Trypanosoma rangeli by lectins in combination with complement Iysis. Acta Tropica 41: 29-38.
Tobie, E. J., 1964. Increased infectivity of a cyclically maintained strain of Trypanosoma rangeli to Rhodnius prolixus and mode of transmission by invertebrate host. J. Parasitol. 50: 593-598.
Tobie, E. J., 1968. The relation of Trypanosoma rangeli to its vector. In Medicina Tropical (Ed. A. Anselmi), Talleres gráficos de Editorial Fournier, México, p. 291-305.
Vallejo, G. A., 1984. Diferenciación entre las formas de desarrollo de Trypanosoma cruzi y las formas de desarrollo de Try panosoma rangeli en el intestino del vector Rhodnius prolixus. Thesis M. Sc. Universidad de los Andes Bogotá, Colombia. 335 pp.
Zeledón, R. & E. Monge. 1966. Natural immunity of the bug, Triatoma infestans to the protozoan Trypanosoma rangeli J. Invertebr. Pathol. 8: 420-423.
Comments
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1986 Revista de Biología Tropical