de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

Phagodeterrence of extracts of Brugmansia candida (Solanaceae) on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae.

Maricruz Arguedas, Luko Hilje, Víctor Cartín, Marco A. Calvo, Henry Borbón



Hypsipyla grandella (Zeller) is a key forest pest in Latin America and the Caribbean, where it has precluded attempts to establish commercial plantations of mahoganies (Swietenia spp.) and cedars (Cedrela spp.). In the search for methods to manage its larvae, being both preventative and cost-effective, as well as based upon resources from tropical flora, the crude flower extract of angel's trumpet, Brugmansia candida Pers., as well as four partitions thereof (water, hexane, dichlorometane, and ethyl acetate), were tested for phagodeterrence. Laboratory bioassays involved increasing concentrations of the crude extract (0.1, 0.3, 1.0, 3.2 and 10.0 % w/v) as well as each one of the partitions (in accordance to the yield obtained from the partitioning process). A randomized complete block design, with four replicates, was used. H. grandella instar III larvae were exposed for 24 h to Spanish cedar (Cedrela odorata) leaf discs dipped into the respective treatment, after which disc consumption was measured. Strong phagodeterrence was detected at concentrations as low as 1.0 % y 0.122 % w/v for the crude extract and the hexane partitions, respectively. In addition, a single bioassay was performed to compare a single concentration (0.38 % w/v) of the alkaloid scopolamine, which reduced consumption in 90 % with respect to the controls. Also, mortality and sublethal effects were assessed in larvae exposed to the crude extract, the hexane partition and scopolamine, with no evidence for them, which confirmed their phagodeterrence. Moreover, the crude extract was submitted to a phytochemical screening by means of a number of qualitative tests, which showed that alkaloids, tannins, triterpenes and cumarins were the most important secondary metabolites. Finally, the presence of scopolamine was confirmed in both the crude extract and the hexane partition, by means of the gas chromatography-spectrometry (GC-MS) analytical method. It is recommended to prepare a formulation of scopolamine plus some adjuvants, in order to test its effectiveness under greenhouse conditions, as a first step to advance in the development of a commercial phagodeterrent.


Hypsipyla grandella, mahogany shoot borer, Brugmansia candida, angel´s trumpet, phagodeterrence, scopolamine.


Abbasipour, H., Mahmoudvand, M., Rastegar, F., & Hosseinpour, M. H. (2011). Bioactivities of jimsonweed extract, Datura stramonium L. (Solanaceae), against Tribolium castaneum (Coleoptera: Tenebrionidae). Turkish Journal of Agriculture and Forestry, 35, 623-629.

Alves, M. N, Sartoratto, A., & Trigo, J. R. (2007). Scopolamine in Brugmansia suaveolens (Solanaceae): defense, allocation, costs, and induced response. Journal of Chemical Ecology, 33(2), 297-309.

Arango, G. (2008). Alcaloides y compuestos nitrogenados. Medellín, Colombia: Universidad de Antioquia.

Arteaga de G. L., Pera, M., & Reguero, M. T. (1993). Brugmansia: una especie promisoria para la producción de alcaloides del tropano. Revista Colombiana de Ciencias Químico Farmacéuticas, 21, 36-40.

Barboza, J., Hilje, L., Durón, J., Cartín, V., & Calvo, M. (2010a). Fagodisuasión de un extracto de ruda (Ruta chalepensis, Rutaceae) y sus particiones sobre larvas de Hypsipyla grandella (Lepidoptera: Pyralidae). Revista de Biología Tropical, 58(1), 1-14.

Barboza, J., Hilje, L., Durón, J., Cartín, V., & Calvo, M. (2010b). Actividad fagodisuasiva y sistémica de una formulación derivada de un extracto de ruda (Ruta chalepensis, Rutaceae) sobre larvas de Hypsipyla grandella (Lepidoptera: Pyralidae). Revista de Biología Tropical, 58(1), 15-29.

Becker, V. O. (1976). Microlepidópteros asociados con Carapa, Cedrela y Swietenia. In J. L. Whitmore (Ed.), Studies on the shootborer Hypsipyla grandella (Zeller). Lep. Pyralidae (pp. 75-101). Vol. II. Turrialba, Costa Rica: IICA Miscellaneous Publication No. 101. IICA.

Bruneton, J. (2001). Farmacognosia. Fitoquímica. Plantas medicinales. España: Acribia.

Buchanan, B., Gruissem, W., & Jones, R. (2000). Biochemistry and molecular biology of plants. Chichester, England: Wiley-Blackwell.

Cruz, A., Rodríguez, C. E., & Ortiz, C. (2011). Efecto insecticida in vitro del extracto etanólico de algunas plantas sobre la mosca adulta Haematobia irritans. Revista Cubana de Plantas Medicinales,16(3), 216-226.

Eich, E. (2008). Solanaceae and Convolvulaceae. Secondary metabolites: biosynthesis, chemotaxonomy, biological and economics significance. Berlin: Springer-Verlag Berlin Heidelberg.

Gómez, L. D. (1996). Vegetación de Costa Rica. EUNED. San José, Costa Rica.

Hilje, L., & Cornelius, J. (2001). ¿Es inmanejable Hypsipyla grandella como plaga forestal? Manejo Integrado de Plagas, No. 61, Hoja Técnica No. 38, p. I-IV.

Hilje, L., & Mora, G. A. (2006). Promissory botanical repellents/deterrents for managing two key tropical insect pests, the whitefly Bemisia tabaci and the mahogany shootborer Hypsipyla grandella. In M. Rai & C. Carpinella (Eds.), Naturally occurring bioactive compounds: a new and safe alternative for control of pests and diseases (pp. 379¬-403). Amsterdam¬: Advances in Phytomedicine, Vol. 3. Elsevier.

InfoStat, Software Estadístico. (2001). Versión 1.0. Estadística y Biometría. Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Argentina.

Mancebo, F., Hilje, L. Mora, G., & Salazar, R. (2000). Antifeedant activity of Quassia amara (Simaroubaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae. Crop Protection, 19(5), 301-305.

Mancebo, F., Hilje, L. Mora, G., Castro, V., & Salazar, R. (2001). Biological activity of Ruta chalepensis (Rutaceae) and Sechium pittieri (Cucurbitaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae. Revista de Biología Tropical, 49(2), 501-508.

Mancebo, F., Hilje, L. Mora, G., & Salazar, R. (2002). Biological activity of two neem (Azadirachta indica A. Juss., Meliaceae) products on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae. Crop Protection, 21(2), 107-112.

Otálvaro, A. M. (2009). Evaluación de un sistema de biorreacción para la producción de escopolamina por cultivo de raíces de Brugmansia candida. (Tesis de Doctorado). Departamento de Ingeniería Química y Ambiental, Universidad Nacional de Colombia. Bogotá, Colombia.

Pérez-Flores, J., Eigenbrode, S. D., & Hilje-Quirós, L. (2012). Alkaloids, limonoids and phenols from Meliaceae species decrease survival and performance of Hypsipyla grandella Larvae. American Journal of Plant Sciences, 3(7), 988-994.

Schabel, H., Hilje, L., Nair, K. S. S., & Varma, R. V. (1999). Economic entomology in tropical forest plantations: An update. Journal of Tropical Forest Science, 11(1), 303-315.

Schoonhoven, L. M. (1980). Perception of azadirachtin by some lepidopterous larvae. In H. Schmutterer, K. Ascher, & H. Rembold (Eds.). Natural pesticides from the neem tree (Azadirachta indica A. Juss) (p. 105-108). Germany: GTZ. Eschborn.

Sharapin, N., Rochas, L. M., Carvalho, E. S., de Albuquerque, E. M. R., Almeida, J. M. L., & Pinzón, R.S. (Eds.). (2000). Fundamentos de tecnología de productos fitoterapéuticos. Convenio Andrés Bello (CAB). Bogotá, Colombia: Programa Iberoamericano de Ciencias y Tecnología para el Desarrollo (CYTED).

Soto, F., Hilje, L., Mora, G., Aguilar M. E., & Carballo, M. (2007). Systemic activity of plant extracts in Cedrela odorata (Meliaceae) seedlings and their biological activity on Hypsipyla grandella (Lepidoptera: Pyralidae) larvae. Agriculture & Forest Entomology, 9(3), 221-226.

Stashenko, E., & Martínez, J. (2012). GC-MS analysis of volatile plant secondary metabolites. In B. Salih & O. Celikbicak (Eds.). Gas Chromatography in Plant Science, Wine Technology, Toxicology and Some Specific Applications (pp. 262-264). Croatia: InTech.

Tarmadi, D., Himmi, S. K., & Yusuf, S. (2014). Termiticidal activity of an extract of Brugmansia candida leaves against a subterranean termite Copotermes gestroi Wasmann and a drywood termite Cryptotermes cynocephalus Light. Proceedings of the 10th Pacific-Termite Research Group Conference, S3:4.

Tomerlin, J., & Howell, T. (1988). Distrain: A computer program for training people to estimate disease severity on cereal leaves. Plant Disease, 72, 455-459.

Vargas, C., Shannon, P., Taveras, R., Soto, F., & Hilje, L. (2001). Un nuevo método para la cría masiva de Hypsipyla grandella. Manejo Integrado de Plagas, No. 62, Hoja Técnica No. 39, p. I-IV.

Wang, S., Liu, L., Wang, L., Hu, Y., Zhang, W., & Liu, R. (2012). Structural characterization and identification of major constituents in Jitai tablets by high-performance liquid chromatography/diode-array detection coupled with electrospray ionization tandem mass spectrometry. Molecules, 17(9), 10470-10493.

Warthen, D. (1990). Insect feeding deterrents (1976-1980). Part A. In E. Morgan & N. Mandava (Eds.), CRC Handbook of Natural Pesticides Vol VI, Insect attractants and repellents (pp. 23-82). Florida: CRC Press, Boca Raton, Inc.


  • There are currently no refbacks.

© 2017 Universidad de Costa Rica. Para ver más detalles sobre la distribución de los artículos en este sitio visite el aviso legal. Este sitio es desarrollado por UCRIndex y Open Journal Systems. ¿Desea cosechar nuestros metadatos? dirección OAI-PMH: