Abstract
The four most important pathogens of coffee trees in Costa Rica, Mycella citricolor, Phoma costarricensis, Cercospora coffeicola, and Ceratostomella fimbriata were grown in liquid cultures and on agar plates to test their responses to eleven different vitamins and to thirteen aminoacids separately and combined, in terms of growth (expressed as oven dry weight and diametral growth on plates) and of spoculation. None of the vitamins used improved growth significantly of Mycella citricolor, Phoma costarricensis or Cercospora coffeicola. Ceratostomella fimbriata grew best in thiamine and nucleic acid. Mycella citricolor and Ceratostomella fimbriata sporulated in thiamine only. Phoma costarricensis spoculated in five of the eleven vitamins used. The four organisms were favored in their growth by the presence of amino acids. L-tryptophane, L-valine and asparagin were the only amino acids that induced spoculation on the three fungi, Mycella citricolor, Phoma costarricensis and Ceratostomella fimbriata. Ceratostomella fimbriata did not grow in a mixture of amino acids.Comments
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