Keywords
oocytes
sperm
in vitro fertilization
gamete quality
Ciprinidae
ornamental aquaculture
barbo tigre
ovocitos
espermatozoides
fertilización in vitro
calidad de gametos
Ciprinidae
acuacultura ornamental
How to Cite
Abstract
The production of ornamental fishes represents an economic activity of a growing number of Mexican families. Nevertheless, the reproduction of fish in captivity is one of the complications faced by farmers. This study was set up to: (i) evaluate the morphological and functional changes induced by hydration in the gametes of fish tiger barb (Puntius tetrazona; 240 samples) at tree times after hydration (10, 20 and 30s) with classic spermograms (volume, sperm concentration, viability, motility, and normal morphology); and (ii) evaluate the implementation of in vitro fertilization based on the ovulation rate, the percentage of fertilization and hatching, and the larval numbers obtained after 72 hours. The average volume of milt was 3.0±0.7μL, and the minimum, maximum and average concentration of sperm was 44.4x106 spz/mL, 52.3x106spz/mL, and 48.1±5.9x106spz/mL, respectively. The viability and motility of the sperm was 84.6±3.2% and 81.5±2.2%, respectively. The diameter of the sperm with/without water contact was 2.1±0.6μm and 3.8±1.0μm (p<0.05); the largest diameter was recorded 30 seconds after the contact with water. For oocytes, the smaller and larger diameters were recorded at 10 and 30s, respectively (both with/without water contact); the oocytes diameters after 10 and 30 seconds of contact with water were 1.11 and 1.55mm, respectively. A higher ovulation rate was recorded using the in vitro fertilization: 250±50 oocytes versus 28±09 oocytes (during natural fertilization; p<0.05). Nevertheless, fertilization and hatching rates were higher for the natural fertilization (80 and 60%, respectively). Considering the number of larvae obtained after 72 hours, our results showed a higher value for the in vitro fertilization (75±18 compared to 13.4±12 of the natural fertilization; p<0.05). We propose this fish as a model for other ornamental fishes of commercial interest. Our results demonstrate that the in vitro fertilization is a very high viable option to optimize and maximize resources; besides, the reproduction management optimization under controlled conditions may enhance wild fish stocks preservation.References
Beirão, J., Soares, F., Herráez, M. P., Dinis, M. T., & Cabrita, E. (2009). Sperm quality evaluation in Solea senegalensis during the reproductive season at cellular level. Theriogenology, 72, 1251-1261.
Billard, R., Cosson, J., Crim, L. W., & Suquet, M. (1995a). Sperm physiology and quality. In N. Bromage & R. Roberts (Eds.), Broodstock management and egg and larval quality (pp. 25-52). Oxford, Reino Unido: Blackwell.
Billard, R., Cosson, G., Perchec, G., & Linhart, O. (1995b). Biology of sperm and artificial reproduction in carp. Aquaculture, 129, 95-112.
Blaxter, J. H. (1969). Development: eggs and larvae. In W. S. Hoar, & D. J. Randal (Eds.), Fish Physiology (pp. 177). New York, US: Academic Press.
Bobe, J. & Labbé, C. (2010). Egg and sperm quality in fish. General and Comparative Endocrinology, 165, 535-548.
Bromage, N. & Roberts, J. R. (1995). Broodstock management and egg and larval quality. Oxford, Reino Unido: Blackwell Science.
Clemens, H. P. & Grant, F. B. (1985). The seminal thinning response of carp (Cyprinus carpio) and rainbow trout (Salmo gairdnerii) after injections of pituitary extracts. Copeia, 2, 174-177.
Coward, K., Bromage, N. R., Hibbitt, O., & Parrington, J. (2002). Gametogenesis, fertilization and egg activation in teleost fish. Reviews in Fish Biology and Fisheries, 12, 33-58.
Dawes, J. (2005). Complete encyclopedia of the freshwater aquarium. Canada and US: Firefly Book.
Domínguez-Castanedo, O. (2012). Los Sistemas Acuícolas de Recirculación: ¿una alternativa para el cultivo sustentable de peces ornamentales en el Estado de Morelos? Sociedades Rurales, Producción y Medio Ambiente, 12(24), 207-225.
Donaldson, M. E. (1996). Manipulation of reproduction in farmed fish. Animal Reproduction Science, 42, 381-392.
Dos Anjos, J. D. B., Siquiera, J. A., & Amorim, R. M. S. (2007). Comercio de peixes ornamentais do Estado de Amazonas, Brasil. Sociedade Brasileira de Ictiologia, 87, 4-5.
Hajirezaee, S., Amiri, B. M., & Mirvaghefi, A. (2010). Fish milt quality and major factors influencing the milt quality parameters: A review. African Journal of Biotechnology, 9(54), 9148-9154.
Hoang, M., Kyu, H., Hwa, B., Seon, M., Hyun, M., Uie, J., & Jin, Y. (2010). Effects of varying dilutions, pH, temperature and cations on spermatozoa motility in fish Larimichthys polyactis. Journal of Environmental Biology, 32, 271-276.
Kime, D. E., Van Look, K. J. W., Mcallister, B. G., Huysekens, G., Rarangwa, E., & Ollevier, F. (2001). Computer-assisted sperm analysis CASA as a tool for monitoring sperm quality in fish. Comparative Bioquemistry and Physiology, part C, 130, 425-433.
Kruger, J. C., Smit, G. L., Van Vuren, J. H. J., & Ferreira. T. J. (1984). Some chemical and physical characteristics of the semen of Cyprinus carpio L. and Oreochromis mossambicus (Peters). Journal of Fish Biology, 24, 263-272.
Lahnsteiner, F., Soares, F., Ribeiro, L., & Dinis, T. M. (2009). Egg quality determination in teleost fish. In E. Cabrita, V. Robles, & P. Herraez (Eds.), Methods in reproductive aquaculture (pp. 149-173). US: Taylor & Francis Group, CRC Press.
Lambert, D. 2001. A practical guide to breeding your freshwater fish. Ingland: Barron´s.
Lavara, R., Mocé, E., Lavara, F., Viudes de Castro, M. P., & Vicente, S. J. (2005). Do parameters of seminal quality correlate with the results of an on-farm inseminations rabbit? Theriogenology, 64, 1130-1141.
Linhart, O., Cosson, J., Mims, S. D., Shelton, W. I., & Rodina, M. (2002). Effects of ions on the motility of fresh and demembranated paddlefish (Polyodon spathula) spermatozoa. Reproduction, 124, 713-719.
Martínez-Espinosa, D., Durán, E. E., Erna, C. A., Hernández, E. D., Galván, A., Goletto, A., Martínez, K., Malpica, S. A., Morfin, S. M., Hernández, A. J., & Hernández, A. (2009). Plan Rector para la piscicultura de ornato del Estado de Morelos. SAGARPA.
Martínez-Espinosa, D., Sánchez-Robles, J., Matus-Parada, J., & Binqüist-Cervantes, G. (2013). Análisis de los factores que condicionan la idoneidad de la estructura productiva de las granjas acuícolas de peces de ornato del estado de Morelos. Sociedades Rurales, Producción y Medio Ambiente, 13(25), 93-114.
Metwally, M. A. A. & Fouad, M. I. (2009). Effects of L-Ascorbic Acid on Sperm Viability in Male Grass Carp (Ctenopharyngodon idellus). Global Veterinaria, 3(2), 132-136.
Munro, A. D., Li-Lian, C., & Nqankee, K. (1990). Preliminary observations on environmental control of ovulation and spawning in a small tropical Cyprinid (Barbus tetrazona). Japan: Proceedings of the Second Asian Fish Forum.
Musselius, V. A. (1951). How to store carp milt and to determine its quality. Rybnoe Khozyaistvo, 27, 51-53.
Mylonas, C. C., Fostier, A., & Zanuy, S. (2010). Broodstock management and hormonal manipulations of fish reproduction. General and Comparative Endocrinology, 165, 516-534.
Ohta, T. & Matsuda, M. (1995). Sperm morphology and IMP distribution in membranes of spermatozoa of cyprinid fishes. Cell Structure and Function, 20, 293-300.
Petrovický, I. (2002). Peces de acuario. España: Tikal Ediciónes.
Rodríguez, G. M. (1992). Técnicas de evaluación cuantitativa de la madurez gonádica en peces. México: A.G.T. Editor, S.A.
Rurangwa, E., Kime, D. E., Ollevier, F., & Nash, P. J. (2004). The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234, 1-28.
Springate, J. & Bromage, R. N. (1985). Effect of egg size on early growth and survival in rainbow trout (Salmo gairdneri Richardson). Aquaculture, 47, 163.
Tabares, J. C., Tarazona, A. M., & Olivera, M. A. (2005). Fisiología de la activación del espermatozoide en peces de agua dulce. Revista Colombiana de Ciencias Pecuarias, 18(2), 149-161.
Takai, H. & Morisawa, M. (1995). Changes in intracelular K+ concentrations caused by external osmolarity change regulate sperm motility of marine and freshwater teleosts. Journal of Cell Science, 108, 1175-1181.
Van Look, K. J. K. & Kime, E. D. (2003). Automated sperm morphology analysis in fishes: the effect of mercury on goldfish sperm. Journal of Fish Biology, 63, 1020-1033.
Vladic, T. V., Afzelius, B., & Bronnikov, E. G. (2002). Sperm quality as reflected through morphology in salmon alternative life histories. Biology of Reproduction, 66, 98-105.
Vlok, W. & Van Vuren, J. H. J. (1988). Physical composition of the semen of Barbus aeneus, the smallmouth yellowfish (Cyprinidae). Comparative Biochemical Physiology, 90(3), 387-389.
Xiao, Q., Zhang, S., Hu, J., & Xu, Y. (2006). Sperm of rosy barb (Puntius conchonius) as an in vitro assay system of nonylphenol cytotoxicity. Journal of Environmental Science, 18(3), 417-419.
Yamamoto, T. (1961). Physiology of fertilization in fish eggs. International Review of Cytology, 12, 361-405.
Zohar, Y. & Mylonas, C.C. (2001). Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture, 197, 99-136.
Comments
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2014 Revista de Biología Tropical