Plant regeneration of indica rice <i>(Oryza sativa)</i> cultivars fmm mature embryo=derived calli

Abstract

Plant regeneration from seven-week-old callus cultures derived from mature embryos of several indica rice cu!tivars was achieved with frequencies of morphogenic calli from 10 lo 47 %. Three media were tested both for callogenesis and plant regeneration. For 3 of the 7 genotypes examined, the best combination of media for plant regeneration was Murashige & Skoog basal medium: MSC (callogenesis) and MSR (regeneration). The rates of callogenesis were not related to the capacity for plant regeneration. Two genolypes CR- l l l 3 and CR-5272 produced the highest number of regenerated green plants. The results of this sludy suggest that genetic differences could be directly linked to the ability to regenerate in these plant cultivars.