Keywords
dormancy
Hechtia perotensis
seed
seed vigour
tetrazolium test.
Bromeliaceae
Hechtia perotensis
latencia
semilla
tetrazolio
vigor de semilla
How to Cite
Abstract
Endemic populations of Hechtia perotensis have been described in Puebla and Veracruz, Mexico. Good quality seed collections can be used in conservation, research and ecological restoration. To evaluate seed quality of wild and endemic species, some compounds are used as effective promoters of germination, such as potassium nitrate (KNO3) and gibberellic acid (AG3), because they increase seed germination capacity and reduce latency. The triphenyl tetrazolium chloride (tetrazolium) test correlates seed viability because it is based on the activity of dehydrogenases in live tissues that catalyze mitochondrial respiration. The objective of this study was to obtain information on size and weight of capsules and seeds and seed germination and viability of H. perotensis, collected in Veracruz in the year 2012 and 2015. The hypotheses were 1) that seed germination and viability are independent of the year of collection, 2) that there is a tetrazolium concentration that can identify seed viability better than others, and 3) that pretreatment with KNO3 or AG3 improves seed germination. Seed germination was assessed using a completely randomized design with three treatments (control and the germination promoters 0.2 % KNO3 and 500 mg/L AG3), four treatments for the viability test (control, 0.2, 0.5 and 1.0 % of tetrazolium) and six replicates for each treatment. A total of one hundred seeds for germination experiments, and 25 seeds for the viability test were used. The results between and within years were analyzed with ANOVA and multiple comparison with the Tukey test. The proportion of non-germinated seeds was quantified along with the number of normal and abnormal seedlings, seeds with viable embryo, seeds without embryo, and seeds with low or no viability. On average, for the 2012 collected sample, 36 % had viable embryos, 7 % had low viability, 24 % were not viable and 33 % had no embryo. This result was significantly different from the 2015 sample, for which 87 % of seed showed viable embryos, 10 % had low viability, 0 % was not viable and 3 % had no embryo. Seed germination was also significantly different between years (22 and 92 %) Pregerminative treatments did not improve germination. Seed germination and viability of H. perotensis significantly varied between years of seed collection.
References
Amador-Alférez, K. A., Díaz-González, J., Loza-Cornejo, S., & Bivián-Castro, E. Y. (2013). Efecto de diferentes reguladores de crecimiento vegetal sobre la germinación de semillas y desarrollo de plántulas de dos especies de Ferocactus (Cactaceae). Polibotánica, 35, 109-131.
Baskin, C. C., & Baskin, J. M. (2008). Advances in understanding seed dormancy at the whole-seed level: An ecological, biogeographical and phylogenetic perspective. Acta Botanica Yunnanica, 30, 279-294.
Bewley, J. D. (1997). Seed germination and dormancy. The Plant Cell, 9, 1055-1066.
Craviotto, R. M., Arango, P. M. R., & Gallo, C. (2011). Novedades de la Prueba de Viabilidad por Tetrazolio en Soja. Santa Fe, Argentina: Instituto Nacional de Tecnología Agropecuaria. Laboratorio de semillas EEA Oliveros.
Davière, J. M., & Achard, P. A. (2013). Gibberellin signaling in plants. Development, 140, 1147-1151.
Deno, N. C. (1994). The critical role of gibberellins in germination and survival of certain cacti. Cactus and Succulent Journal, 66, 28-30.
Donohue, K., Rubio de Casas, R., Burghardt, L., Kovach, K., & Willis, C. G. (2010). Germination, postgermination adaptation, and species ecological ranges. Annual Review of Ecology, Evolution, and Systematic, 41, 293-319.
El‑Maarouf‑Bouteau, H., & Bailly, C. (2008). Oxidative signaling in seed germination and dormancy. Plant Signaling & Behavior, 3, 175-182.
Escobedo, S., J. (2012). Mecanismos de dispersión de semillas en las Bromelias. Desde el herbario CICY 4: 22-23. http://www.cicy.mx/sitios/desde_herbario/
Espejo-Serna, A., López-Ferrari, A. R., Ramírez-Morillo, I., & Martínez-Correa, N. (2007). Dos nuevas especies de Hechtia (Bromeliaceae) de México. Acta Botánica Mexicana, 78, 97-109.
Finch-Savage, W. E., & Leubner-Metzger, G. (2006). Seed dormancy and the control of germination. New Phytologist, 171, 501-23.
Finkelstein, R. R., Gampala, S. S., & Rock, C. D. (2002). Abscisic acid signaling in seeds and seedlings. The Plant Cell Supplement, 14, 15-45.
Furatani, S. C., & Nagao, M. A. (1987). Influence of temperature, KNO3, AG3 and seed drying on emergence of papaya seedlings. Scientia Horticulturae, 32, 67-72.
García, E. (1988). Modificaciones al sistema de clasificación climática de Köppen. México: Universidad Nacional Autónoma de México.
García, F. A., Montes, H. S., Rangel, L. J. A., García, M. E., & Mendoza, E. M. (2010). Respuesta fisiológica de la semilla chile piquín [Capsicum annuum var. glabriusculum (Dunal) Heiser & Pickersgill] al ácido giberélico e hidrotermia. Revista Mexicana de Ciencias Agrícolas, 1, 203-216.
Givnish, T. J., Millam, K. C., Berry, P. E., & Sytsma, K. J. (2007). Phylogeny, adaptive radiation, and historical biogeography of Bromeliaceae inferred from ndhf sequence data. Aliso, 23, 3-26.
Glenner, G. G. (1990). Formazans and tetrazolium salts. En R. D. Lillie (Ed.), Biological Stains (pp. 225-235). St. MO: Sigma Chemical Company.
International Seed Testing Association (2010). International Rules for Seed Testing. Basserdorf, CH Switzerland.
Lima, D. S., Lima, D. I., Venezuela, G. R., & Macias, P. (1985). Estudio de la viabilidad de las semillas de Carica papaya L. (Variedad de Maradol Roja). Centro Agrícola, 12, 119-130.
Llorente-Bousquets, J., & Ocegueda, S. (2008). Estado del conocimiento de la biota, en Capital natural de México (vol. I, pp. 283-322). Conocimiento actual de la biodiversidad. México: Conabio.
Magnitskiy, S. V. & Ligarreto, G. A. (2007). Efecto del nitrato de potasio, del ácido giberélico y del ácido indolacético sobre la germinación de semillas de agraz (Vaccinium meridionale Swartz). Revista Colombiana de Ciencias Hortícolas, 1, 137-141.
Mandujano, M. C., Golubov, J., & Rojas-Aréchiga, M. (2007). Efecto del ácido giberélico en la germinación de tres especies del género Opuntia (Cactaceae) del Desierto Chihuahuense. Cactáceas y Suculentas Mexicanas, 52, 46-52.
Montes, R. S. (2013). Germinación de especies de la familia Bromeliaceas: aspectos ecológicos y anatómicos (Tesis de Doctorado). Universidad Autónoma de México, México.
Navarrete, R. J. A. (1996). Producción del Papayo (Carica papaya L.) Variedad Maradol en Yucatán. Instituto Tecnológico Agropecuario No. 2. Mérida, Yucatán. México: Centro de Investigación y Graduados Agropecuarios. Dirección General de Educación Tecnológica Agropecuaria.
Peña-Valdivia, C. B., Sánchez-Urdaneta, A. B., Aguirre, R. J. R., Trejo, C., Cárdenas, E., & Villegas, M. A. (2006). Temperature and mechanical scarification on seed germination of “maguey” (Agave salmiana Otto ex Salm-Dyck). Seed Science and Technology, 34, 47-56.
Pereira, A. R., Andrade, A. C. S., Pereira, T. S., Forzza, R. C., & Rodríguez, A. S. (2010). Morphological aspects of seed, germination and storage of Pitcairnia albiflos (Bromeliaceae). Seed Science and Technology, 38, 79-87.
Pons, T. L., & Poorte, H. (2014). The effect of irradiance on the carbon balance and tissue characteristics of five herbaceous species differing in shade-tolerance. Frontiers in Plant Science, 5, 1-14.
Ramírez, M. I., Jiménez, C. F., Fernández-Concha, G. C., & Pinzón, J. P. (2014). Three new species and growth patterns in Hechtia (Bromeliaceae: Hechtioideae). Phytotaxa, 178, 113-127.
Rzedowski, J. (1991). El endemismo de la flora fanerogámica mexicana: una apreciación analítica preliminar. Acta Botánica Mexicana, 15, 47-64.
Reyes, S. J. (2015). Conservación y restauración de cactáceas y otras plantas suculentas mexicanas. México: Comisión Nacional Forestal.
Saldívar-Iglesias, P., Laguna-Cerda, A., Gutiérrez-Rodríguez, F., & Domínguez-Galindo, M. (2010). Ácido giberélico en la germinación de semillas de Jaltomata procumbens (Cav.) J. L. Gentry. Agronomía Mesoamericana, 21, 327-331.
Sarukhán, J., Halffter, G., & Koleff, P. (2009). Capital natural de México. Síntesis: conocimiento actual, evaluación y perspectivas de sustentabilidad. México: Comisión Nacional para el Conocimiento y Uso de la Biodiversidad.
Siadat, S. A., Moosavi, S. A., Zadeh, M. S., Fotouhi, F., & Zirezadeh, M. (2011). Effects of halo and phytohormone seed priming on germination and seedling growth of maize under different duration of accelerated ageing treatment. African Journal of Agricultural Research, 6, 6453-6462.
Sosa, L. D., Chávez, S. J. L., Mondragón, Ch. D., Estrada, G. J. A., & Ramírez, V. P. (2012). Viabilidad y germinación de semillas de seis especies de Tillandsia (Bromeliaceae) de Oaxaca, México. Fitotecnia Mexicana, 35, 37-42.
Tigabu, M., & Oden, P. C. (2001). Effect of scarification, gibberellic acid and temperature on seed germination of two multipurpose Albizia species from Ethiopia”. Seed Science and Technology, 29, 11-20.
Toledo, A. T., & Wolf, J. H. D. (2008). Germination and establishment of Tillandsia eizii (Bromeliaceae) in the canopy of an oak forest in Chiapas, Mexico. Biotropica, 40, 246-250.
Victoria, J., Bonilla, C., & Sánchez, M. (2006). Viabilidad en tetrazolio de semillas de caléndula y eneldo. Acta Agronómica, 55, 14-18.
Wenny, D. G. (2000). Seed dispersal, seed predation, and seedling recruitment of a neotropical montane tree. Ecological Monograph, 70, 331-351.
Xiao, Z. S., Harris, M. K., & Zhang, Z. B. (2007). Acorn defenses to herbivory from insects: implications for the joint evolution of resistance, tolerance and escape. Forest Ecology and Management, 238, 302-308.
Zheng, C., Jiang, D., Liub, F., Dai, T., Liu, W., Jing, Q., & Cao, W. (2009). Exogenous nitricoxide improves seed germination in wheat against mitochondrial oxidative damage induced by high salinity. Environmental and Experimental Botany, 67, 222-227.
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