Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

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Descripción de un método simple y económico para el estudio de cariotipos en serpientes
Vol. 23 No. 2 (1975), Articles
Vol. 23 No. 2 (1975)

Descripción de un método simple y económico para el estudio de cariotipos en serpientes

Articles
Published December 1, 1975
R. Taylor
Universidad de Costa Rica
R. Bolaños
Universidad de Costa Rica
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Taylor, R., & Bolaños, R. (1975). Descripción de un método simple y económico para el estudio de cariotipos en serpientes. Revista De Biología Tropical, 23(2), 177–183. Retrieved from https://revistas.ucr.ac.cr/index.php/rbt/article/view/25984
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Abstract

We describe a method for the preparation of snake chromosomes in metaphase, based on the in vivo stimulation of leucocytes with crude phytohemagglutinin from Phaseolus lunatus and the in vitro blocking of mitosis with colchicine. It has the advantage of preserving the specimen alive without the complications of cell culture, and can be performed under field conditions.
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References

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BAKER, R. J., G. A. MENGDEN, & J. J. BULL 1972. Karyotypic studies of thirty-eight species of North American snakes. Copeia, 1972: 257-265.

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TJIO, J. H., & J. WHANG 1960. Chromosome preparations of bone marrow cells without prior in vitro culture or in vivo colchicine administration. Stain Techn., 37: 17-20.

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