Abstract
Embryonated cricken eggs were inoculated with Sporotrichum schenckii by ten different routes: yolk sac, chorio-allantoic membrane, allantoic, extra embryonary, and amniotic cavities, albumen sac, brain, muscle, blood vessels, and shell membrane. Five eggs were used in each type of inoculation. Detailed mycological studies and anatomo-pathologic microscopic examination were carried out, using P.A.S., Gram and hematoxyilin-eosin techniques.
The fungus behaved differently in the various tissues and fluids of the embryo. Pleomorphism was observed, including vegetative mycelium, reproductive mycelium, pseudomycelium, promycelium, blastospore production with one or Several buds, small and large conidia, rounded and cigar-shaped cells. No asteroid formations were found.