Revista de Biología Tropical ISSN Impreso: 0034-7744 ISSN electrónico: 2215-2075

OAI: https://revistas.ucr.ac.cr/index.php/rbt/oai
Critical vitrification steps towards survival of Garcinia hombroniana (Clusiaceae) shoot tips after cryopreservation
PDF
HTML

Keywords

cryopreservation
vitrification steps
Garcinia hombroniana
shoot tip
Malaysia.
crioconservación
vitrificación
Garcinia hombroniana
ápice caulinar
Malasia.

How to Cite

Sulong, N., Shahabudin, N. F., & Mohd Noor, N. (2018). Critical vitrification steps towards survival of Garcinia hombroniana (Clusiaceae) shoot tips after cryopreservation. Revista De Biología Tropical, 66(3), 1314–1323. https://doi.org/10.15517/rbt.v66i3.31483

Abstract

A cryopreservation protocol was developed for in vitro shoot tips of Garcinia hombroniana using the vitrification technique. Four critical steps in the technique were investigated, namely preculture, loading, dehydration with Plant Vitrification Solution 2 (PVS2), and unloading. Shoot tips precultured for 48 hr gave significantly higher survival (75 %) compared to 24 hr preculture (50 %) after cryopreservation. Treatment with 1 M glycerol plus 0.4 M sucrose as a loading solution gave higher survival (45.83 %) compared to the other treatments (0.4 M sucrose + 2 M glycerol; 0.4 M sucrose). Shoot tips dehydrated with PVS2 for 25 min gave the highest survival after immersion in liquid nitrogen. Stepwise PVS2 treatment for 15 min with 50 % PVS2 followed by 10 min with 100 % PVS2 solution improved survival of the shoot tips after cryopreservation (41.67 %). Murashige and Skoog medium with 0.4 M sucrose gave significantly higher survival (66.67 %) than MS with 1.2 M sucrose (25 %) as an unloading solution. Water content was shown to decrease throughout the whole vitrification steps from 6.83 ± 1.66 g g-1 dw for fresh shoot tips down to 2.93 ± 0.28 g g-1 dw after PVS2 treatment. Further study on each step including recovery medium is required to improve the survival. Nevertheless, the present study showed the potential of using the vitrification technique for cryopreservation of G. hombroniana.

https://doi.org/10.15517/rbt.v66i3.31483
PDF
HTML

References

Abraham, Z., Senthilkumar, R., John, K. J., Sharma, T. V. R. S., Nair, N. V., Unnikrishnan, M., Kumaran, P. M., George, J. K., Uma, S., Latha, M., Malik, S. S., Mishra, S. K., Bhandari D. C., & Pareek S. K. (2008). Collection of plant genetic resources from Andaman and Nicobar Islands. Genetic Resources Crop Evolution, 55, 1279-1289.

Chen, X. L., Li, J. H., Xin, X., Zhang, Z. E., Xin P. P., & Lu. X. X. (2011). Cryopreservation of in vitro-grown apical meristems of Lilium by droplet-vitrification. South African Journal of Botany, 77, 397-403.

Chua, S. P., & Normah, M. N. (2011). Effect of preculture, PVS2 and vitamin C on survival of recalcitrant Nephelium ramboutan-ake shoot tips after cryopreservation by vitrification. CryoLetters, 32, 506-515.

Hong, S., Yin, M., Shao, X., Wang, A., & Xu, W. (2009). Cryopreservation of embryogenic callus of Dioscorea bulbifera by vitrification. CryoLetters, 30, 64-75.

Hong, S. R., & Yin, M. H. (2009). High-efficiency vitrification protocols for cryopreservation of in vitro grown shoot tips of rare and endangered plant Emmenopterys henryi Oliv. Plant Cell, Tissue and Organ Culture, 99, 217-226.

Ibrahim, S., & Normah, M. N. (2013). The survival of in vitro shoot tips of Garcinia mangostana L. after cryopreservation by vitrification. Plant Growth Regulation, 70, 237-246.

Ishikawa, K., Harata, K., Mii, M., Sakai, A., Yoshimatsu, K., & Shimomura, K. (1997). Cryopreservation of zygotic embryos of a Japanese terrestrial orchid (Bletilla striata) by vitrification. Plant Cell Reports, 16, 754-757.

Kim, H. H., Cho, E. G., Baek, H. J., Kim, C. Y., Keller, E. R. J., & Engelmann, F. (2004). Cryopreservation of garlic shoot tips by vitrification: Effects of dehydration, rewarming, unloading and regrowth conditions. CryoLetters, 25, 59-70.

Langis, R., & Steponkus, P. L. (1990). Cryopreservation of rye protoplasts by vitrification. Plant Physiology, 92, 666-671.

Matsumoto, T., & Sakai, A. (2003). Cryopreservation of axillary shoot tips of in vitro-grown grape (Vitis) by a two-step vitrification protocol. Euphytica, 131, 299-304.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473-497.

Nadarajan, J., Mansor, M., Krishnapillay, B., Staines, H. J., Benson E. E., & Harding K. (2008). Applications of differential scanning calorimetry in developing cryopreservation strategies for Parkia speciosa, a tropical tree producing recalcitrant seeds. CryoLetters, 29, 95-110.

Nazre, M. (2010). Historical review and notes on the correct scientific name for seashore mangosteen. Genetic Resources Crop Evolution, 57, 1249-1259.

Nazre, M., Clyde, M. M., & Latiff, A. (2007). Phylogenetic relationships of locally cultivated Garcinia species with some wild relatives. Malaysian Applied Biology, 36, 31-40.

Niu, Y. L., Zhang, Y. F., Zhang Q. L., & Luo, Z. R. (2010). A preliminary study on cryopreservation protocol applicable to all types of Diospyros kaki Thunb. Biotechnology & Biotechnological Equipment, 24, 1960-1964.

Noor, N. M. (2008). Rahsia dan misteri biji benih tropika spesies rekalsitran. Penerbit Universiti Kebangsaan Malaysia.

Pennycooke, J. C., & Towill, L. E. (2000). Cryopreservation of shoot tips from in vitro plants of sweet potato [Ipomoea batatas (L.) Lam.] by vitrification. Plant Cell Reports, 19, 733-737.

Rao, N. K. (2004). Plant genetic resources: Advancing conservation and use through biotechnology. African Journal of Biotechnology, 3, 136-145.

Reed, B. M. (2008). Cryopreservation-practical considerations. In B. M. Reed (Eds), Plant Cryopreservation: A Practical Guide (pp. 3-13). Springer: New York, USA.

Sakai, A., & Engelmann F. (2007). Vitrification, encapsulation-vitrification and droplet-vitrification: A review. CryoLetters, 28, 151-172.

Sakai, A., Hirai D., & Niino, T. (2008). Development of PVS-based vitrification and encapsulation-vitrification protocols. In B. M. Reed (Eds), Plant Cryopreservation: A Practical Guide (pp. 33-57). Springer: New York, USA.

Sakai, A., Kobayashi S., & Oiyama, I. (1990). Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Reports, 9, 30-33.

San José, M. C., Valladares, S., Janeiro, L. V., & Corredoira, E. (2014). Cryopreservation of in vitro-grown shoot tips of Alnus glutinosa (L.) Gaertn. Acta Physiologiae Plantarum, 36, 109-116.

Sinniah, U. R., & Gantait, S. (2013). Cryopreservation of immature Parkia speciosa Hassk. zygotic embryonic axes following desiccation or exposure to vitrification solution. Acta Physiologiae Plantarum, 35, 2629-2634.

Turner, S., Senaratna, T., Touchell, D., Bunn, E., Dixon, K., & Tan, B. (2001). Stereochemical arrangement of hydroxyl groups in sugar and polyalcohol molecules as an important factor in effective cryopreservation. Plant Science, 160, 489-497.

Varghese, D., Berjak, P., & Pammenter, N. W. (2009). Cyopreservation of shoot tips of Trichilia emetica, a tropical recalcitrant-seeded species. CryoLetters, 30, 280-290.

Volk, G. M., & Walters, C. (2006). Plant vitrification solution 2 lowers water content and alters freezing behavior in shoot tips during cryoprotection. Cryobiology, 52, 48-61.

Walters, C., Berjak, P., Pammenter, N., Kennedy K., & Raven, P. (2013). Preservation of recalcitrant seeds. Science, 339, 915-916.

Wesley-Smith, J., Vertucci, C. W., Berjak, P., Pammenter N. W., & Crane, J. (1992). Cryopreservation of desiccation-sensitive axes of Camellia sinensis in relation to dehydration, freezing rate and the thermal properties of tissue water. Journal of Plant Physiology, 140, 596-604.

Yaacob, O., & Tindall, H. D. (1995). Mangosteen cultivation. FAO Plant Production and Protection Paper 129, Rome, Italy.

Yap, L. V., Noor, N. M., Clyde, M. M., & Chin, H. F. (2011). Cryopreservation of Garcinia cowa shoot tips by vitrification: The effects of sucrose preculture and loading treatment on ultrastructural changes in meristematic cells. CryoLetters, 32, 188-196.

Zhang, J. M., Han, L., Lu, X. X., Volk, G. M., Xin, X., Yin, G. K., He, J. J., Wang, L., & Chen, X. L. (2017). Cryopreservation of Jerusalem artichoke cultivars using an improved droplet-vitrification method. Plant Cell, Tissue and Organ Culture, 128, 577-587.

Comments

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2018 Revista de Biología Tropical

Downloads

Download data is not yet available.