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Resumen
Plant structures that secrete lipids and phenolic compounds are often associated with the protection and development of organs against desiccation, in addition to the protection they provide against animals, as the capitate trichomes of Adenocalymma magnificum. Understanding the glandular activities that occur in these trichomes has required the study of their ontogeny, structure, ultrastructure and histochemical aspects; the interpretation of their ecological functions or evolutionary history is complicated by the scarcity of reports on calicinal trichomes that are not nectar-secreting. Samples of floral calyx in anthesis and flower buds at different stages of development were fixed and processed according to the methods for light and electron microscopy. The trichomes are randomly distributed throughout the entire inner surface of the calyx and are also visible on the flower buds. These capitate glandular trichomes were composed of a peduncle, having up to nine cells, and a multicellular secretory head with their cells in columnar format and arranged in disc form. The collar cell, which is under the secretory head, divides anticlinally and arranges itself side by side with the mother cell. As they develop, they bend with some of them becoming adpressed to the calyx. Histochemical tests indicate that the secretory head cells produce lipid substances, acidic lipids and phenolic compounds. In the secretory head, the vacuome is dispersed and the cytoplasm possesses a great number of smooth endoplasmic reticulum and leucoplasts, organelles involved in the production of osmiophilic substances. In some regions of the secretory cells, cuticle detachment was observed; however, the accumulation of secretions was not observed. This study describes, for the first time, the origin, development, and secretion process of the calicinal trichomes of Adenocalymma magnificum, showing that production of lipophilic substances is important for this plant, possibly the trichomes may be involved in the plant’s chemical defense against insects, offering protection against herbivores.
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Ascensão, L., Marques, N., & Pais, M. (1995). Glandular trichomes on vegetative and reproductive organs of Leonotis leonurus (Lamiaceae). Annals of Botany, 75, 619-626.
Ascensão, L., Mota, L., & Castro, M. M. (1999). Glandular trichomes on the leaves and flowers of Plectranthus ornatus: morphology, distribution and histochemistry. Annals of Botany, 84, 437-447.
Ascensão, L., & Pais, M. S. S. (1987). Glandular Trichomes of Artemisia campestris (ssp. Maritima): ontogeny and histochemistry of the secretory product. Botanical Gazette, 148, 221-227.
Bottega, S., & Corsi, G. (2000). Structure, secretion and possible functions of calyx glandular hairs of Rosmarinus officinalis L. (Labiatae). Botanical Journal of the Linnean Society, 132, 325-335.
Cain, A. J. (1947). The use of Nile blue in the examination of lipoids. Quarterly Journal of Microscopical Science, 88, 383-392.
Castro, M. A., Vega, A. S., & Múlgura, M. E. (2001). Structure and ultrastructure of leaf and calyx glands in Galphimia brasiliensis (Malpighiaceae). American Journal of Botany, 88, 1935-1944.
Combrinck, S., Du Plooy, G. W., McCrindle, R. I., & Botha, B. M. (2007). Morphology and histochemistry of the glandular trichomes of Lippia scaberrima (Verbenaceae). Annal of Botany, 99, 1111-1119.
Croteau, R., Kutchan, T. M., & Lewis, N. G. (2000). Natural Products (Secondary Metabolites). In B. Buchanan, W. Gruissem, & R. Jones (Eds.), Biochemistry & Molecular Biology of Plants (pp. 1250-1318). Rockville: American Society of Plant Physiologists.
Fahn, A. (1979). Secretory tissues in plants. London: Academic Press.
Fahn, A. (2000). Structure and function of secretory cells. Advances in Botanical Research, 31, 37-75.
Favorito, S. (2009). Tricomas secretores de Lippia stachyoides Cham. (Verbenaceae): estrutura, ontogênese e secreção (Master thesis). Universidade Estadual Paulista. Retrieved from http://base.repositorio.unesp.br/bitstream/handle/11449/88132/favorito_s_me_botib.pdf?sequence=1&isAllowed=y
Gerlach, D. (1984). Botanische Mikrotechnik. Stuttgart: Georg Thieme.
Hayat, M. A. (1972). Basic electron microscopy techniques. New York: VanNostrand Reinhold.
Johansen, D. A. (1940). Plant microtechnique. New York: McGraw-Hill Book Company Inc.
Jurišić Grubešić, R., Vladimir-Knežević, S., Kremer, D., Kalodera, Z., & Vuković, J. (2007). Trichome micromorphology in Teucrium (Lamiaceae) species growing in Croatia. Biologia, 62, 148-156.
Machado, S. R., Gregório, E. A., & Guimarães, E. (2006). Ovary peltate trichomes of Zeyheria montana (Bignoniaceae): developmental ultrastructure and secretion in relation to function. Annals of Botany, 97, 357-369.
McManus, J. F. A. (1948). Histological and histochemical uses of periodic acid. Stain Technology, 23, 99-108.
Moura, M. Z. D., Isaias, R. M. S., & Soares, G. L. G. (2005). Ontogenesis of internal secretory cells in leaves of Lantana camara (Verbenaceae). Botanical Journal of the Linnean Society, 148, 427-431.
Paiva, E. A. S., & Martins, L. C. (2011). Calycinal trichomes in Ipomoea cairica (Convolvulaceae): ontogenesis, structure and functional aspects. Australian Journal of Botany, 59, 91-98.
Paiva, E. A. S. (2009). Ultrastructure and post-floral secretion of the pericarpial nectaries of Erythrina speciosa (Fabaceae). Annals of Botany, 104, 937-44.
Pearse, A. G. E. (1980). Histochemistry, Theoretical and Applied: Preparative and optical technology. London: Churchill Livingstone.
Reynolds, E. S. (1963). The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology, 17, 208-212.
Rivera, G. L. (2000a). Nuptial nectary structure of Bignoniaceae of Argentina. Darwiniana, 38, 227-239.
Rivera, G. L. (2000b). Nectarios extranupciales florales en especies de Bignoniaceae de Argentina. Darwiniana, 38, 1-10.
Robards, A. W. (1978). An introduction to techniques for scanning electron microscopy of plant cells. In J. L. Hall (Ed.), Electron Microscopy and Cytochemistry of Plant Cells (pp. 343-444). New York: Elsevier.
Roland, J. C. (1978). General preparations and staining of thin sections. In J. L. Hall (Ed.), Electron Microscopy and Cytochemistry of Plant Cells (pp. 1-63). New York: Elsevier.
Sacchetti, G., Romagnoli, C., Nicoletti, M., Di Fabio, A., Bruni, A., & Poli, F. (1999). Glandular Trichomes of Calceolaria adscendens Lidl. (Scrophulariaceae): Histochemistry, Development and Ultrastructure. Annals of Botany, 83, 87-92.
Schmid, K. M., & Ohlrogge, J. B. (2002). Lipid metabolism in plants. In D. E. Vance, & J. E. Vance (Eds.), Biochemistry of Lipids, Lipoproteins and Membranes (pp. 93-126). Amsterdam: Elsevier.
Seibert, R. J. (1948). The Use of Glands in a Taxonomic Consideration of the Family Bignoniaceae. Annals of the Missouri Botanical Garden, 35, 123-137.
Silva, E. M. J., & Machado, S. R. (1999). Estrutura e desenvolvimento dos tricomas secretores em folhas de Piper regnellii (Miq.) C. DC. var. regnellii (Piperaceae). Brazilian Journal of Botany, 22, 117-124.
Simões, A. O., Castro, M. M., & Kinoshita, L. S. (2006). Calycine colleters of seven species of Apocynaceae (Apocynoideae) from Brazil. Botanical Journal of the Linnean Society, 152, 387-398.
Solereder, H. (1908). Systematic Anatomy of the Dicotyledons. Oxford: Clarendon Press.
Subramanian, R. B., & Inamdar, J. A. (1985). Occurrence, Structure, Ontogeny and Biology of Nectaries in Kigelia pinnata DC. Botanical Magazine, 98, 67-73.
Subramanian, R. B., & Inamdar, J. A. (1989). The structure, secretion and biology of nectaries in Tecomaria capensis Thunb (Bignoniaceae). Phytomorphology, 39, 69-74.
Thomson, W. M., Platt-Aloia, K. A., & Endress, A. G. (1976). Ultrastructure of Oil Gland Development in the Leaf of Citrus sinensis L. Botanical Gazette, 137, 330-340.
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Derechos de autor 2015 Revista de Biología Tropical