Selection of native strains of Rhizobium leguminosarum bv phaseoli efficient in biological nitrogen fixation in Costa Rican soils.
DOI:
https://doi.org/10.15517/am.v6i0.24808Abstract
One hundred and thirty one strains of R. leguminosarum bv phaseoli were isolated from different bean producing zones in Costa Rica. The response of its inoculation to the Huasteco Negro (Phaseolus vulgaris L.) bean variety was evaluated under green house conditions. Pots and heat treated soil were used. The seeds inoculation was conducted using a bacterial suspension of 10° ucf/ml, approximately. Besides, anabsolute control (without inoculation, without nitrogen) and a control with nitrogen (+ 140 kg N/ha) were included. The dry weight of the nodules, dry weight of the aerial part and the ureids concentration of the foliar area at the R6 stage ofdevelopment were the measured variables. The plants inoculated with the CR-455, CR-487 and CR-BSA strains showed the largest nodules’ dry weight values. The absolute control showed the lowest dry weight of nodules. Significant differences (P<0.05) were observed among so me treatments and the controls. The plants showing the highest foliar dry weight were those inoculated with the CR-482, CR-492, CR- 422 and CR-454 strains. Significant differences (P<0.05) were registered for the ureids concentrations among some treatments and the controls. The plants inoculated with the CR-475, CR- 492 and CR-422, CR-487 and CR-477 strains had the highest ureids concentrations and also showed the largest atmospheric nitrogen fixing capacity under green house conditions. A variation of the atmospheric nitrogen fixing capacity or effectiveness among some Rhizobium strains was observed, when comparing the results with the absolute and nitrogenous controls, which agrees with the bibliography.
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ACUÑA, O. 1991. Manejo y tecnología en la asociación simbiótica Rhizobium-Ieguminosa. In: Curso de Microbiología de Suelos. San José, Universidad de Costa Rica, Centro de Investigaciones Agronómicas. 4p.
CASTRO, L.; ACUÑA, O. 1992. Determinación de la concentración de ureídos en tres leguminosas fijadoras de nitrógeno: soya, frijol y maní. Agronomía Costarricense 16(2): 187-193.
DEBOUCK, D.; HIDALGO, R. 1985. Morfología de la planta de frijol común. In Frijol: Investigación y producción. Ed. por M. López, F. Fernández y A. Schoonhoven. Cali, Colombia, CIAT - PNUD. p.7-42.
GRAHAM, P. 1981. Some problems of nodulation and symbiotic nitrogen fixation in Phaseolus vulgaris L: a review. Field Crops Research 4: 93-112.
HAN, P. 1981. Some problems of nodulation and symbiotic nitrogen fixation in common bean. Agronomy Journal 71(6):925-926.
McCLURE, P.; ISRAEL, D. 1979. Transport of nitrogen in the xilem of soybean plant. Plant Physiology 64:411-416.
MORALES, M. 1987. Selección y evaluación de cepas de Rhizobium leguminosarum biovar phaseoli tolerantes al suministro restringido de fósforo. Tesis Mag. Sc. Turrialba, Costa Rica, UCR/CATIE. 97p.
MUMNS, D.N. 1987. Nitrogen fixation potential of bean (P. vulgaris) compared with other grains legumes under controlled conditions. Plant and Soil 98(2): 169-182.
PIHA,M.; MUMNS,D. 1987. Sensitivityofthecommon bean (P. vulgaris) symbiosis to high soil temperature. Plant and Soil 98(2): 183-194.
ROSAS, J. 1983. Principios y prácticas para la conducción de ensayos sobre fijación de nitrógeno en condiciones de campo. Ceiba 27(1):23-39.
ROSAS, J.; BLISS, F. 1986. Utilización del potencial de fijación de nitrógeno del frijol común en Centro América. Ceiba 27: 105-115.
SILVESTER, R.; KIPE, J.; HARRIS, D. 1987. Simbiosis leguminosa-rizobio: Evaluación, selección y manejo. Centro Internacional de Agricultura Tropical. Cali, Colombia. 72 p.
SILVESTER, R. 1983. Fijación biológica de nitrógeno por leguminosas: aspectos agronómicos relacionados con su nodulación con Rhizobium. Suelos Ecuatoriales 13(2):28-35.
SOMASEGARAN, P.; HOBEN, H.; HALLIDA Y, J. 1981. Ejercicios prácticos en tecnología Rhizobium-Ieguminosa. Curso regional de entrenamientos sobre la simbiosis entre Rhizobium y leguminosas tropicales de importancia agrícola. Centro de Investigaciones Agronómicas, Facultad de Agronomía. Universidad de Costa Rica. 85 p.
SOTO, G. 1992. Geographic distribution and enviromental interactions that affect the abundance of Rhizobium leguminosarum biovar phaseoli in Costa Rica. M.Sc. Thesis Department of Environmental Sciences, University of Virginia. 207p .
SPRENT, J. 1980. Root nodule anatomy, type of export product and evolutionary origen of so me leguminosae. Plant Cell Environ. 3:35-43.
URIBE, L.; ACUÑA, O.; HERNANDEZ, G. 1990. Respuesta de Phaseolus vulgaris var. Negro Huasteco a la inoculación con tres cepas de Rhizobium bajo condiciones de mínima labranza en tres localidades de Costa Rica. Agronomía Costarricense 14(2):201-206.
URIBE, L.; ACUÑA, O. 1987. Efecto de la fertilización y tipo de suelo sobre la inoculación con diferentes cepas de Rhizobium leguminosarum biovar phaseoli en frijol común. In: Primera Reunión Centroamericana de Fijación Biológica de Nitrógeno. Guatemala. 67 p.
VOGELES, G. VAN DER DRIFT. 1970. Differential analyses of glyoxylate derivatives. Analytical Biochemistry 33:143-157.
ZAMORA, A.; ACUÑA, O. 1991. Mejoramiento de la fijación biológica de nitrógeno en el frijol común. In: Proyectos Regionales de Investigación. Informes anuales. Profrijol. 369 p.
ZAMORA, A.; ACUÑA, O.; RODRIGUEZ, J. 1990. Mejoramiento de la fijación biológica del nitrógeno en el frijol. In: Proyectos Regionales de Investigación. Planes de trabajo 1990-1992. Profrijol. 173 p.
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